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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-January Volume 44 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis

  • Authors:
    • Li Tai Fang
    • Sharon Lee
    • Helen Choi
    • Hong Kwan Kim
    • Gregory Jew
    • Hio Chung Kang
    • Lin Chen
    • David Jablons
    • Il-Jin Kim
  • View Affiliations / Copyright

    Affiliations: Thoracic Oncology Laboratory, Department of Surgery, University of California, San Francisco, San Francisco, CA, USA, Life Technologies, Seattle, WA, USA
  • Pages: 211-221
    |
    Published online on: October 25, 2013
       https://doi.org/10.3892/ijo.2013.2150
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Abstract

We performed whole exome sequencing and gene expression analysis on a metastatic colon cancer to the lung, along with the adjacent normal tissue of the lung. Whole exome sequencing uncovered 71 high-confidence non‑synonymous mutations. We selected 16 mutation candidates, and 13 out of 16 mutations were validated by targeted deep sequencing using the Ion Torrent PGM customized AmpliSeq panel. By integrating mutation, copy number and gene expression microarray data, we identified a JAZF1 mutation with a gain-of-copy, suggesting its oncogenic potential for the lung metastasis from colon cancer. Our pathway analyses showed that the identified mutations closely reflected characteristics of the metastatic site (lung) while mRNA gene expression patterns kept genetic information of its primary tumor (colon). The most significant gene expression network was the ‘Colorectal Cancer Metastasis Signaling’, containing 6 (ADCY2, ADCY9, APC, GNB5, K-ras and LRP6) out of the 71 mutated genes. Some of these mutated genes (ADCY9, ADCY2, GNB5, K-ras, HDAC6 and ARHGEF17) also belong to the ‘Phospholipase C Signaling’ network, which suggests that this pathway and its mutated genes may contribute to a lung metastasis from colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fang LT, Lee S, Choi H, Kim HK, Jew G, Kang HC, Chen L, Jablons D and Kim I: Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis. Int J Oncol 44: 211-221, 2014.
APA
Fang, L.T., Lee, S., Choi, H., Kim, H.K., Jew, G., Kang, H.C. ... Kim, I. (2014). Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis. International Journal of Oncology, 44, 211-221. https://doi.org/10.3892/ijo.2013.2150
MLA
Fang, L. T., Lee, S., Choi, H., Kim, H. K., Jew, G., Kang, H. C., Chen, L., Jablons, D., Kim, I."Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis". International Journal of Oncology 44.1 (2014): 211-221.
Chicago
Fang, L. T., Lee, S., Choi, H., Kim, H. K., Jew, G., Kang, H. C., Chen, L., Jablons, D., Kim, I."Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis". International Journal of Oncology 44, no. 1 (2014): 211-221. https://doi.org/10.3892/ijo.2013.2150
Copy and paste a formatted citation
x
Spandidos Publications style
Fang LT, Lee S, Choi H, Kim HK, Jew G, Kang HC, Chen L, Jablons D and Kim I: Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis. Int J Oncol 44: 211-221, 2014.
APA
Fang, L.T., Lee, S., Choi, H., Kim, H.K., Jew, G., Kang, H.C. ... Kim, I. (2014). Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis. International Journal of Oncology, 44, 211-221. https://doi.org/10.3892/ijo.2013.2150
MLA
Fang, L. T., Lee, S., Choi, H., Kim, H. K., Jew, G., Kang, H. C., Chen, L., Jablons, D., Kim, I."Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis". International Journal of Oncology 44.1 (2014): 211-221.
Chicago
Fang, L. T., Lee, S., Choi, H., Kim, H. K., Jew, G., Kang, H. C., Chen, L., Jablons, D., Kim, I."Comprehensive genomic analyses of a metastatic colon cancer to the lung by whole exome sequencing and gene expression analysis". International Journal of Oncology 44, no. 1 (2014): 211-221. https://doi.org/10.3892/ijo.2013.2150
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