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Article

Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells

  • Authors:
    • Myeongjin Yi
    • Paramasivam Parthiban
    • Jiyoung Hwang
    • Xin Zhang
    • Hyunjin Jeong
    • Dong Ho Park
    • Dong-Kyoo Kim
  • View Affiliations / Copyright

    Affiliations: Department of Chemistry and Institute of Basic Sciences, Inje University, Gimhae 621-749, Republic of Korea
  • Pages: 327-335
    |
    Published online on: November 1, 2013
       https://doi.org/10.3892/ijo.2013.2162
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Abstract

The present study was carried out to investigate the effect of 2,4,6,8-tetraaryl-3,7-diazabicyclo[3.3.1]nonan-9-one (bispidinone) analogs on the in vitro growth of human cervical carcinoma (HeLa) cells. A series of 11 bispidinone analogs was synthesized with substituents, e.g., fluoro/methyl/ethyl/isopropyl/thiomethyl/methoxy groups, at various positions. These compounds were synthesized to identify which substituent and position induced the strongest cytotoxic effect in cancer cells. Among these synthetics, analog 9, which contains methoxy groups, had the most significant cytotoxic effect on HeLa cells, and its IC50 value was less than 13 µM. A WST-8 assay also showed that analog 9 inhibited the proliferation of HeLa cells. By using DNA content analysis, we found that analog 9 induced sub-G1 and G1 phase arrest in a time‑dependent manner. A [3H]-thymidine incorporation assay suggested that analog 9 inhibited DNA replication in HeLa cells. On performing light microscopy, morphological changes such as cellular shrinkage and disruption, which are apoptotic features, were observed in HeLa cells. Annexin V/propidium iodide double staining and rhodamine-123 staining showed that analog 9 induced apoptosis and disrupted the intracellular mitochondrial membrane potential in HeLa cells. The western blot analysis results suggested that analog 9 induced mitochondria-mediated apoptosis. In addition, we have shown that analog 9 may play a role in the Fas signaling apoptotic pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yi M, Parthiban P, Hwang J, Zhang X, Jeong H, Park DH and Kim D: Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells. Int J Oncol 44: 327-335, 2014.
APA
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D.H., & Kim, D. (2014). Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells. International Journal of Oncology, 44, 327-335. https://doi.org/10.3892/ijo.2013.2162
MLA
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D. H., Kim, D."Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells". International Journal of Oncology 44.1 (2014): 327-335.
Chicago
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D. H., Kim, D."Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells". International Journal of Oncology 44, no. 1 (2014): 327-335. https://doi.org/10.3892/ijo.2013.2162
Copy and paste a formatted citation
x
Spandidos Publications style
Yi M, Parthiban P, Hwang J, Zhang X, Jeong H, Park DH and Kim D: Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells. Int J Oncol 44: 327-335, 2014.
APA
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D.H., & Kim, D. (2014). Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells. International Journal of Oncology, 44, 327-335. https://doi.org/10.3892/ijo.2013.2162
MLA
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D. H., Kim, D."Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells". International Journal of Oncology 44.1 (2014): 327-335.
Chicago
Yi, M., Parthiban, P., Hwang, J., Zhang, X., Jeong, H., Park, D. H., Kim, D."Effect of a bispidinone analog on mitochondria‑mediated apoptosis in HeLa cells". International Journal of Oncology 44, no. 1 (2014): 327-335. https://doi.org/10.3892/ijo.2013.2162
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