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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-January Volume 44 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research

  • Authors:
    • Akira Komiya
    • Tomonori Kato
    • Takashi Hori
    • Junya Fukuoka
    • Kenji Yasuda
    • Hideki Fuse
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama, Japan, Laboratory of Pathology, Toyama University Hospital, Toyama, Japan, Department of Pathology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  • Pages: 195-202
    |
    Published online on: November 13, 2013
       https://doi.org/10.3892/ijo.2013.2173
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Abstract

Tissue microarrays were constructed using prostate needle biopsy specimens obtained from 58 patients who underwent radical prostatectomy for localized or locally advanced prostate cancer (PC). We used the spiral array (SA) technique, a novel approach for tissue array construction in a spiral form, which has advantages over small needle biopsy specimens. Roll-shaped tissue pieces produced by slicing a prostate biopsy tissue block and trimming the cancer segment were used to obtain a tissue array block. Cancer segments measuring >3 mm were incorporated into the tissue arrays. Cancer fragments (n=253) were obtained from formalin-fixed, paraffin-embedded needle biopsy specimens. The median number of cancer fragments per patient was four (1-8, min-max). On SA, the median number of confirmed cancer fragments per patient was four (1-7) and 224 cancer fragments (88.5%) were confirmed histologically. Each core of reeled tissue contained at least one cancer fragment. The expressions of multiple prognostic molecular markers for PC (Ki-67, p53 and bcl-2) were immunohistochemically measured using the SA. The Ki-67 and bcl-2 expressions were significantly associated with the Gleason score (GS). A univariate analysis identified Ki-67, bcl-2 and GS as significant predictors of cancer-specific survival, p53 and bcl-2 as significant predictors of overall survival and Ki-67, adjuvant androgen deprivation and GS as significant predictors of biochemical progression. In a multivariate analysis, p53 was independently associated with overall survival, while adjuvant androgen deprivation and GS were associated with biochemical progression. These results indicate that SA has potential as a new tool for translational research on PC.
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Copy and paste a formatted citation
Spandidos Publications style
Komiya A, Kato T, Hori T, Fukuoka J, Yasuda K and Fuse H: Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research. Int J Oncol 44: 195-202, 2014.
APA
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., & Fuse, H. (2014). Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research. International Journal of Oncology, 44, 195-202. https://doi.org/10.3892/ijo.2013.2173
MLA
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., Fuse, H."Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research". International Journal of Oncology 44.1 (2014): 195-202.
Chicago
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., Fuse, H."Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research". International Journal of Oncology 44, no. 1 (2014): 195-202. https://doi.org/10.3892/ijo.2013.2173
Copy and paste a formatted citation
x
Spandidos Publications style
Komiya A, Kato T, Hori T, Fukuoka J, Yasuda K and Fuse H: Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research. Int J Oncol 44: 195-202, 2014.
APA
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., & Fuse, H. (2014). Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research. International Journal of Oncology, 44, 195-202. https://doi.org/10.3892/ijo.2013.2173
MLA
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., Fuse, H."Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research". International Journal of Oncology 44.1 (2014): 195-202.
Chicago
Komiya, A., Kato, T., Hori, T., Fukuoka, J., Yasuda, K., Fuse, H."Application of a new technique, spiral tissue microarrays constructed using needle biopsy specimens, to prostate cancer research". International Journal of Oncology 44, no. 1 (2014): 195-202. https://doi.org/10.3892/ijo.2013.2173
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