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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-March Volume 44 Issue 3

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer

  • Authors:
    • Mitsuhiro Nakamura
    • Xiuzhi Zhang
    • Yasunari Mizumoto
    • Yoshiko Maida
    • Yukiko Bono
    • Masahiro Takakura
    • Satoru Kyo
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920-8641, Japan
  • Pages: 669-677
    |
    Published online on: December 23, 2013
       https://doi.org/10.3892/ijo.2013.2230
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Abstract

A small subset of cells with CD133 expression is thought to have increased chemoresistance and tumorigenicity, features of cancer stem cells (CSCs); the molecular mechanisms by which these properties arise remain unclear. We characterized CD133+ endometrial cancer cells based on microarray analyses of Ishikawa cells. Of the genes upregulated in CD133+ cells compared with CD133- cells, we noted several key factors involved in the aggressive behavior of cells, including ABCG2 and matrix metalloproteinase (MMP). Flow cytometric analyses identified a side-cell population (SP) with CSC features in Ishikawa cells, and they were found to be more enriched in CD133+ cells than CD133- cells. In particular, CD133+/SP cells exhibited higher proliferative and colony‑forming activity than CD133+/non-SP cells. Matrigel invasion assay revealed that CD133+ cells have enhanced invasive capacity with elevated MT1-MMP expression. siRNA‑based knockdown of MT1-MMP largely abolished the invasive capacity of CD133+ cells, but not CD133- cells due to low levels of constitutive MT1-MMP1 expression. These findings demonstrate that increased chemoresistance and tumorigenic potential of CD133+ cells are at least partly attributed to an enriched SP fraction as well as increased MMP-1 expression. These results will be of assistance in the establishment of molecular target therapy to CSCs in endometrial cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Nakamura M, Zhang X, Mizumoto Y, Maida Y, Bono Y, Takakura M and Kyo S: Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer. Int J Oncol 44: 669-677, 2014.
APA
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., & Kyo, S. (2014). Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer. International Journal of Oncology, 44, 669-677. https://doi.org/10.3892/ijo.2013.2230
MLA
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., Kyo, S."Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer". International Journal of Oncology 44.3 (2014): 669-677.
Chicago
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., Kyo, S."Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer". International Journal of Oncology 44, no. 3 (2014): 669-677. https://doi.org/10.3892/ijo.2013.2230
Copy and paste a formatted citation
x
Spandidos Publications style
Nakamura M, Zhang X, Mizumoto Y, Maida Y, Bono Y, Takakura M and Kyo S: Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer. Int J Oncol 44: 669-677, 2014.
APA
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., & Kyo, S. (2014). Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer. International Journal of Oncology, 44, 669-677. https://doi.org/10.3892/ijo.2013.2230
MLA
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., Kyo, S."Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer". International Journal of Oncology 44.3 (2014): 669-677.
Chicago
Nakamura, M., Zhang, X., Mizumoto, Y., Maida, Y., Bono, Y., Takakura, M., Kyo, S."Molecular characterization of CD133+ cancer stem-like cells in endometrial cancer". International Journal of Oncology 44, no. 3 (2014): 669-677. https://doi.org/10.3892/ijo.2013.2230
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