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Article

Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression

  • Authors:
    • Hai Zhang
    • Shanyu Cheng
    • Min Zhang
    • Xiuping Ma
    • Li Zhang
    • Yipin Wang
    • Rong Rong
    • Juan Ma
    • Shukai Xia
    • Mingzhan Du
    • Feng Shi
    • Jie Wang
    • Qinyi Yang
    • Xiaoming Bai
    • Jing Leng
  • View Affiliations / Copyright

    Affiliations: Cancer Center, Department of Pathology, Nanjing Medical University, Nanjing 210029, P.R. China
  • Pages: 769-780
    |
    Published online on: December 30, 2013
       https://doi.org/10.3892/ijo.2013.2234
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Abstract

Prostaglandin E2 (PGE2) has been implicated in hepatocellular carcinoma cell invasion. Recently, it was reported that Y box-binding protein 1 (YB-1) is closely correlated with malignancy. This study was designed to examine the mechanisms by which PGE2 increases YB-1 expression and promotes HCC cell invasion. PGE2 greatly enhanced HCC cell invasion through upregulation of the YB-1 protein, and the EP1 receptor is mainly responsible for this regulation. Src and EGFR were both activated by PGE2, which in turn increased the phosphorylation levels of p44/42 MAPK. Src, EGFR and p44/42 MAPK were all involved in PGE2-induced YB-1 expression. Chemical inhibitors and RNAi analysis all confirmed the role of mTOR complex 1 in YB-1 expression induced by PGE2. Furthermore, YB-1 was able to regulate the expression of a series of EMT-associated genes, which indicated that YB-1 could have the potential to control the epithelial-mesenchymal transition process in HCC cells. These findings reveal that PGE2 upregulated YB-1 expression through the EP1/Src/EGFR/p44/42 MAPK/mTOR pathway, which greatly enhanced HCC cell invasion. This study for the first time describes the mechanisms through which PGE2 regulates YB-1 expression and promotes HCC cell invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Cheng S, Zhang M, Ma X, Zhang L, Wang Y, Rong R, Ma J, Xia S, Du M, Du M, et al: Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression. Int J Oncol 44: 769-780, 2014.
APA
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y. ... Leng, J. (2014). Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression. International Journal of Oncology, 44, 769-780. https://doi.org/10.3892/ijo.2013.2234
MLA
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y., Rong, R., Ma, J., Xia, S., Du, M., Shi, F., Wang, J., Yang, Q., Bai, X., Leng, J."Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression". International Journal of Oncology 44.3 (2014): 769-780.
Chicago
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y., Rong, R., Ma, J., Xia, S., Du, M., Shi, F., Wang, J., Yang, Q., Bai, X., Leng, J."Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression". International Journal of Oncology 44, no. 3 (2014): 769-780. https://doi.org/10.3892/ijo.2013.2234
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Cheng S, Zhang M, Ma X, Zhang L, Wang Y, Rong R, Ma J, Xia S, Du M, Du M, et al: Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression. Int J Oncol 44: 769-780, 2014.
APA
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y. ... Leng, J. (2014). Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression. International Journal of Oncology, 44, 769-780. https://doi.org/10.3892/ijo.2013.2234
MLA
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y., Rong, R., Ma, J., Xia, S., Du, M., Shi, F., Wang, J., Yang, Q., Bai, X., Leng, J."Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression". International Journal of Oncology 44.3 (2014): 769-780.
Chicago
Zhang, H., Cheng, S., Zhang, M., Ma, X., Zhang, L., Wang, Y., Rong, R., Ma, J., Xia, S., Du, M., Shi, F., Wang, J., Yang, Q., Bai, X., Leng, J."Prostaglandin E2 promotes hepatocellular carcinoma cell invasion through upregulation of YB-1 protein expression". International Journal of Oncology 44, no. 3 (2014): 769-780. https://doi.org/10.3892/ijo.2013.2234
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