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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-March Volume 44 Issue 3

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells

  • Authors:
    • Naotake Funamizu
    • Curtis Ray Lacy
    • Sonya T. Parpart
    • Atsushi Takai
    • Yukiharu Hiyoshi
    • Katsuhiko Yanaga
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan, Howard University School of Medicine, Washington DC, USA, Georgetown University, Washington DC, USA, Department of Gastroenterology, Kyoto University, Kyoto, Japan, Department of Surgery, Kumamoto University, Kumamoto, Japan
  • Pages: 725-734
    |
    Published online on: January 7, 2014
       https://doi.org/10.3892/ijo.2014.2243
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Abstract

Recent studies have demonstrated that deregulated microRNA (miR) expression is implicated in the development of human cancers. In the aberrant miR expression, miR-301 is upregulated in cancers, such as pancreatic, colorectal and oral carcinoma. Based on this evidence, we investigated the contribution of miR-301 to pancreatic carcinoma and the novel target genes of miR-301 in pancreatic carcinoma. In this study, we analyzed the effects of enforced and inhibited expression of miR-301b expression in the Panc-1 and BxPC-3 cell lines. MiR-301b expression levels were associated with cell invasiveness in both cell lines. Additional experiments indicated that miR-301b influences invasiveness through CDH1. Moreover microRNA target search algorithms and experimental strategies suggested that miR-301b suppressed TP63 expression as a novel target of miR-301b. Remarkably, miR-301b was also found to be associated with NF-κB activity in both cell lines. In summary, overexpressed miR-301b may suppress TP63 expression and contributes to promote cell invasiveness and to enhance gemcitabine resistance in pancreatic carcinoma cells. Thus, miR-301b may have potential as a novel therapeutic target for cancer treatment due to its stimulatory effects on cell invasiveness.
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Copy and paste a formatted citation
Spandidos Publications style
Funamizu N, Lacy CR, Parpart ST, Takai A, Hiyoshi Y and Yanaga K: MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells. Int J Oncol 44: 725-734, 2014.
APA
Funamizu, N., Lacy, C.R., Parpart, S.T., Takai, A., Hiyoshi, Y., & Yanaga, K. (2014). MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells. International Journal of Oncology, 44, 725-734. https://doi.org/10.3892/ijo.2014.2243
MLA
Funamizu, N., Lacy, C. R., Parpart, S. T., Takai, A., Hiyoshi, Y., Yanaga, K."MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells". International Journal of Oncology 44.3 (2014): 725-734.
Chicago
Funamizu, N., Lacy, C. R., Parpart, S. T., Takai, A., Hiyoshi, Y., Yanaga, K."MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells". International Journal of Oncology 44, no. 3 (2014): 725-734. https://doi.org/10.3892/ijo.2014.2243
Copy and paste a formatted citation
x
Spandidos Publications style
Funamizu N, Lacy CR, Parpart ST, Takai A, Hiyoshi Y and Yanaga K: MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells. Int J Oncol 44: 725-734, 2014.
APA
Funamizu, N., Lacy, C.R., Parpart, S.T., Takai, A., Hiyoshi, Y., & Yanaga, K. (2014). MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells. International Journal of Oncology, 44, 725-734. https://doi.org/10.3892/ijo.2014.2243
MLA
Funamizu, N., Lacy, C. R., Parpart, S. T., Takai, A., Hiyoshi, Y., Yanaga, K."MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells". International Journal of Oncology 44.3 (2014): 725-734.
Chicago
Funamizu, N., Lacy, C. R., Parpart, S. T., Takai, A., Hiyoshi, Y., Yanaga, K."MicroRNA-301b promotes cell invasiveness through targeting TP63 in pancreatic carcinoma cells". International Journal of Oncology 44, no. 3 (2014): 725-734. https://doi.org/10.3892/ijo.2014.2243
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