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Article

Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis

  • Authors:
    • Binbin Huang
    • Qiong Wu
    • Yanli Ge
    • Junjie Zhang
    • Longe Sun
    • Yunyun Zhang
    • Liu Fu
    • Juanjuan Fan
    • Zhirong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China, Department of Gastroenterology of Shanghai Changning Center Hospital, Shanghai 200336, P.R. China
  • Pages: 849-857
    |
    Published online on: January 8, 2014
       https://doi.org/10.3892/ijo.2014.2248
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Abstract

N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes β1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides (N-glycan) of cell proteins and the dysfunction of which is a common feature of various carcinomas. Nevertheless, the role of GnT-V remains controversial. Therefore, the clinical implication of GnT-V expression may differ in each cancer type. The implication of GnT-V status in patients with gastric cancer has not been studied extensively. In the present study, we examined GnT-V expression in gastric cancer specimen both at protein and mRNA levels. We compared GnT-V expression with clinical and pathologic variables. Kaplan-Meier survival curves were generated to show the cause-specific survival. Furthermore, the small interfering RNA was devised to downregulate the GnT-V mRNA expression in SGC7901 and BGC 823 cells. We characterized the function implication of GnT-V by cell proliferation and invasiveness analysis. Analysis in gastric cancer specimen revealed that GnT-V expression correlated with tumor grade and stage. The overall survival time of positive GnT-V expression in gastric cancer was significantly shorter than that of negative GnT-V expression. Moreover, the downregulation of GnT-V expression by small interfering RNA resulted in a decrease of cell proliferation and invasiveness in SGC7901 and BGC 823 cells accompanied by morphological change. This supports that GnT-V correlates with metastasis and prognosis in gastric cancer. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications.
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1. 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2. 

Nagini S: Carcinoma of the stomach: a review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol. 4:156–169. 2012. View Article : Google Scholar : PubMed/NCBI

3. 

Danaei G, Vander Hoorn S, Lopez AD, Murray CJ and Ezzati M; Comparative Risk Assessment collaborating group (Cancers): Causes of cancer in the world: comparative risk assessment of nine behavioural and environmental risk factors. Lancet. 366:1784–1793. 2005. View Article : Google Scholar : PubMed/NCBI

4. 

Catalano V, Labianca R, Beretta GD, Gatta G, de Braud F and Van Cutsem E: Gastric cancer. Crit Rev Oncol Hematol. 71:127–164. 2009. View Article : Google Scholar

5. 

Kim BS, Cho SW, Min SK and Lee BH: Differences in prognostic factors between early and advanced gastric cancer. Hepatogastroenterology. 58:1032–1040. 2011.PubMed/NCBI

6. 

Seshadri RA, Jayanand SB and Ranganathan R: Prognostic factors in patients with node-negative gastric cancer: an Indian experience. World J Surg Oncol. 9:482011. View Article : Google Scholar : PubMed/NCBI

7. 

Qiu MZ, Wang ZQ, Luo HY, et al: Prognostic analysis in node-negative gastric cancer patients in China. Tumour Biol. 32:489–492. 2011. View Article : Google Scholar : PubMed/NCBI

8. 

Murata K, Miyoshi E, Kameyama M, et al: Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis. Clin Cancer Res. 6:1772–1777. 2000.PubMed/NCBI

9. 

Guo P, Chen HJ, Wang QY and Chen HL: Downregulation of N-acetylglucosaminyltransferase V facilitates all-trans retinoic acid to induce apoptosis of human hepatocarcinoma cells. Mol Cell Biochem. 284:103–110. 2005. View Article : Google Scholar : PubMed/NCBI

10. 

Xu YY, Lu Y, Fan KY and Shen ZH: Apoptosis induced by all-trans retinoic acid in N-acetylglucosaminyltransferase V repressed human hepatocarcinoma cells is mediated through endoplasmic reticulum stress. J Cell Biochem. 100:773–782. 2007. View Article : Google Scholar

11. 

Dosaka-Akita H, Miyoshi E, Suzuki O, Itoh T, Katoh H and Taniguchi N: Expression of N-acetylglucosaminyltransferase V is associated with prognosis and histology in non-small cell lung cancers. Clin Cancer Res. 10:1773–1779. 2004. View Article : Google Scholar : PubMed/NCBI

12. 

Ishimura H, Takahashi T, Nakagawa H, et al: N-acetylglucosaminyltransferase V and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer. Clin Cancer Res. 12:2506–2511. 2006. View Article : Google Scholar : PubMed/NCBI

13. 

Inamori K, Gu J, Ohira M, et al: High expression of N-acetylglucosaminyltransferase V in favorable neuroblastomas: involvement of its effect on apoptosis. FEBS Lett. 580:627–632. 2006. View Article : Google Scholar : PubMed/NCBI

14. 

Tian H, Miyoshi E, Kawaguchi N, et al: The implication of N-acetylglucosaminyltransferase V expression in gastric cancer. Pathobiology. 75:288–294. 2008. View Article : Google Scholar : PubMed/NCBI

15. 

Pinho SS, Figueiredo J, Cabral J, et al: E-cadherin and adherens-junctions stability in gastric carcinoma: functional implications of glycosyltransferases involving N-glycan branching biosyn-thesis, N-acetylglucosaminyltransferases III and V. Biochim Biophys Acta. 1830:2690–2700. 2013. View Article : Google Scholar

16. 

Wei T, Liu Q, He F, Zhu W, Hu L, Guo L and Zhang J: The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma. Exp Mol Pathol. 93:8–17. 2012. View Article : Google Scholar : PubMed/NCBI

17. 

Yang HM, Yu C and Yang Z: N-acetylglucosaminyltransferase V negatively regulates integrin α5β1-mediated monocyte adhesion and transmigration through vascular endothelium. Int J Oncol. 41:589–598. 2012.PubMed/NCBI

18. 

Pinho SS, Seruca R, Gärtner F, Yamaguchi Y, Gu J, Taniguchi N and Reis CA: Modulation of E-cadherin function and dysfunction by N-glycosylation. Cell Mol Life Sci. 68:1011–1020. 2011. View Article : Google Scholar : PubMed/NCBI

19. 

Kim YS, Hwang SY, Kang HY, et al: Functional proteomics study reveals that N-Acetylglucosaminyltransferase V reinforces the invasive/metastatic potential of colon cancer through aberrant glycosylation on tissue inhibitor of metalloproteinase-1. Mol Cell Proteomics. 7:1–14. 2008. View Article : Google Scholar

20. 

Abbott KL, Aoki K, Lim JM, et al: Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J Proteome Res. 7:1470–1480. 2008. View Article : Google Scholar : PubMed/NCBI

21. 

Guo HB, Randolph M and Pierce MJ: Inhibition of a specific N-glycosylation activity results in attenuation of breast carcinoma cell invasiveness-related phenotypes: inhibition of epidermal growth factor-induced dephosphorylation of focal adhesion kinase. Biol Chem. 282:22150–22162. 2007. View Article : Google Scholar

22. 

Miyoshi E, Nishikawa A, Ihara Y, et al: N-acetylglucosaminyltransferase III and V messenger RNA levels in LEC rats during hepatocarcinogenesis. Cancer Res. 53:3899–3902. 1993.PubMed/NCBI

23. 

Miyoshi E, Ihara Y, Nishikawa A, et al: Gene expression of N-acetylglucosaminyltransferases III and V: a possible implication for liver regeneration. Hepatology. 22:1847–1855. 1995.PubMed/NCBI

24. 

Yao M, Zhou DP, Jiang SM, Wang QH, Zhou XD, Tang ZY and Gu JX: Elevated activity of N-acetylglucosaminyltransferase V in human hepatocellular carcinoma. J Cancer Res Clin Oncol. 124:27–30. 1998. View Article : Google Scholar : PubMed/NCBI

25. 

Buckhaults P, Chen L, Fregien N and Pierce M: Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. J Biol Chem. 272:19575–19581. 1997. View Article : Google Scholar : PubMed/NCBI

26. 

Lu Y and Chaney W: Induction of N-acetylglucosaminyltran sferase V by elevated expression of activated or proto-Ha-ras oncogenes. Mol Cell Biochem. 122:85–92. 1993. View Article : Google Scholar : PubMed/NCBI

27. 

Pierce M, Buckhaults P, Chen L and Fregien N: Regulation of N-acetylglucosaminyltransferase V and Asn-linked oligosaccharide beta(1,6) branching by a growth factor signaling pathway and effects on cell adhesion and metastatic potential. Glycoconj J. 14:623–630. 1997. View Article : Google Scholar : PubMed/NCBI

28. 

Przybyło M, Pocheć E, Link-Lenczowski P and Lityńska A: Beta 1-6 branching of cell surface glycoproteins may contribute to uveal melanoma progression by up-regulating cell motility. Mol Vis. 14:625–636. 2008.PubMed/NCBI

29. 

Demetriou M, Nabi IR, Coppolino M, Dedhar S and Dennis JW: Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V. J Cell Biol. 130:383–392. 1995. View Article : Google Scholar : PubMed/NCBI

30. 

Seberger PJ and Chaney WG: Control of metastasis by Asnlinked, β-6 branched oligosaccharides in mouse mammary cancer cells. Glycobiology. 9:235–241. 1999.

31. 

Zhao Y, Sato Y, Isaji T, et al: Branched N-glycans regulate the biological functions of integrins and cadherins. FEBS J. 275:1939–1948. 2008. View Article : Google Scholar : PubMed/NCBI

32. 

Gu J and Taniguchi N: Potential of N-glycan in cell adhesion and migration as either a positive or negative regulator. Cell Adh Migr. 2:243–245. 2008. View Article : Google Scholar : PubMed/NCBI

33. 

Isaji T, Kariya Y, Xu Q, Fukuda T, Taniguchi N and Gu J: Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion. Methods Enzymol. 480:445–459. 2010. View Article : Google Scholar : PubMed/NCBI

34. 

Ye Q, Kantonen S and Gomez-Cambronero J: Serum deprivation confers the MDA-MB-231 breast cancer line with an EGFR/JAK3/PLD2 system that maximizes cancer cell invasion. J Mol Biol. 425:755–766. 2013. View Article : Google Scholar : PubMed/NCBI

35. 

Lau KS and Dennis JW: N-Glycans in cancer progression. Glycobiology. 18:750–760. 2008. View Article : Google Scholar : PubMed/NCBI

36. 

Atmaca A, Werner D, Pauligk C, et al: The prognostic impact of epidermal growth factor receptor in patients with metastatic gastric cancer. BMC Cancer. 12:5242012. View Article : Google Scholar : PubMed/NCBI

37. 

Nan BC, Shao DM, Chen HL, Huang Y, Gu JX, Zhang YB and Wu ZG: Alteration of N-acetylglucosaminyltransferases in pancreatic carcinoma. Glycoconj J. 15:1033–1037. 1998. View Article : Google Scholar : PubMed/NCBI

38. 

Shaheen PE, Stadler W, Elson P, Knox J, Winquist E and Bukowski RM: Phase II study of the efficacy and safety of oral GD0039 in patients with locally advanced or metastatic renal cell carcinoma. Invest New Drugs. 23:577–581. 2005. View Article : Google Scholar : PubMed/NCBI

39. 

Taylor JB, Strickland J, May T and Hawkins DE: Effect of subacute swainsonine (locoweed; Oxytropis sericea) consumption on immunocompetence and serum constituents of sheep in a nutrient-restricted state. Vet Hum Toxicol. 42:199–204. 2000.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang B, Wu Q, Ge Y, Zhang J, Sun L, Zhang Y, Fu L, Fan J and Wang Z: Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis. Int J Oncol 44: 849-857, 2014.
APA
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y. ... Wang, Z. (2014). Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis. International Journal of Oncology, 44, 849-857. https://doi.org/10.3892/ijo.2014.2248
MLA
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y., Fu, L., Fan, J., Wang, Z."Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis". International Journal of Oncology 44.3 (2014): 849-857.
Chicago
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y., Fu, L., Fan, J., Wang, Z."Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis". International Journal of Oncology 44, no. 3 (2014): 849-857. https://doi.org/10.3892/ijo.2014.2248
Copy and paste a formatted citation
x
Spandidos Publications style
Huang B, Wu Q, Ge Y, Zhang J, Sun L, Zhang Y, Fu L, Fan J and Wang Z: Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis. Int J Oncol 44: 849-857, 2014.
APA
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y. ... Wang, Z. (2014). Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis. International Journal of Oncology, 44, 849-857. https://doi.org/10.3892/ijo.2014.2248
MLA
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y., Fu, L., Fan, J., Wang, Z."Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis". International Journal of Oncology 44.3 (2014): 849-857.
Chicago
Huang, B., Wu, Q., Ge, Y., Zhang, J., Sun, L., Zhang, Y., Fu, L., Fan, J., Wang, Z."Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis". International Journal of Oncology 44, no. 3 (2014): 849-857. https://doi.org/10.3892/ijo.2014.2248
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