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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-March Volume 44 Issue 3

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway

  • Authors:
    • Xiaoyun Shen
    • Yong Zhang
    • Yan Feng
    • Litu Zhang
    • Jilin Li
    • Yu-An Xie
    • Xiaoling Luo
  • View Affiliations / Copyright

    Affiliations: Research Department, Affiliated Tumour Hospital of Guangxi Medical University, Nanning 530021, P.R. China
  • Pages: 791-796
    |
    Published online on: January 8, 2014
       https://doi.org/10.3892/ijo.2014.2251
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Abstract

Epigallocatechin-3-gallate (EGCG) has been shown to inhibit the growth and induce apoptosis of certain cancer cells. The aim of this study was to determine the role of EGCG in hepatocellular carcinoma (HCC) and the underlying mechanism(s) thereof. MTT assay was used to determine the cell growth inhibition by EGCG. Apoptosis induced by EGCG was investigated by both AO/EB staining and flow cytometry. The cell cycle distribution was analyzed by flow cytometry. The mRNA levels of the AKT pathway were analyzed by quantitative PCR. The expression of AKT and its phosphorylation at Ser473 were detected by western blotting. The IC50 of EGCG at 48 h for HepG2, SMMC7721 and SK-hep1 cells were 74.7, 59.6 and 61.3 µg/ml, respectively. Significantly higher proportion of SMMC7721 cells entered the S phase upon treatment with EGCG for 48 h compared with control cells. EGCG decreased the mRNA levels of PI3K, AKT and NF-κB. The protein levels of AKT decreased and its phosphorylation at Ser473 was downregulated with EGCG treatment. EGCG inhibited growth by affecting the cell cycle and induced apoptosis in different HCC cells by downregulating PI3K/AKT activity. The results suggest the potential of EGCG as an anticancer agent in the prevention or treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Shen X, Zhang Y, Feng Y, Zhang L, Li J, Xie Y and Luo X: Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway. Int J Oncol 44: 791-796, 2014.
APA
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., & Luo, X. (2014). Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway. International Journal of Oncology, 44, 791-796. https://doi.org/10.3892/ijo.2014.2251
MLA
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., Luo, X."Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway". International Journal of Oncology 44.3 (2014): 791-796.
Chicago
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., Luo, X."Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway". International Journal of Oncology 44, no. 3 (2014): 791-796. https://doi.org/10.3892/ijo.2014.2251
Copy and paste a formatted citation
x
Spandidos Publications style
Shen X, Zhang Y, Feng Y, Zhang L, Li J, Xie Y and Luo X: Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway. Int J Oncol 44: 791-796, 2014.
APA
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., & Luo, X. (2014). Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway. International Journal of Oncology, 44, 791-796. https://doi.org/10.3892/ijo.2014.2251
MLA
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., Luo, X."Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway". International Journal of Oncology 44.3 (2014): 791-796.
Chicago
Shen, X., Zhang, Y., Feng, Y., Zhang, L., Li, J., Xie, Y., Luo, X."Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway". International Journal of Oncology 44, no. 3 (2014): 791-796. https://doi.org/10.3892/ijo.2014.2251
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