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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May-2014 Volume 44 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells

  • Authors:
    • Elizabeth E. Sweeney
    • Ping Fan
    • V. Craig Jordan
  • View Affiliations / Copyright

    Affiliations: Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA
  • Pages: 1529-1538
    |
    Published online on: March 6, 2014
       https://doi.org/10.3892/ijo.2014.2329
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Abstract

Models of long-term estrogen-deprived breast cancer cells are utilized in the laboratory to mimic clinical aromatase inhibitor-resistant breast cancer and serve as a tool to discover new therapeutic strategies. The MCF-7:5C and MCF-7:2A subclones were generated through long-term estrogen deprivation of estrogen receptor (ER)-positive MCF-7 cells, and represent anti-hormone‑resistant breast cancer. MCF-7:5C cells paradoxically undergo estrogen-induced apoptosis within seven days of estrogen (estradiol, E2) treatment; MCF-7:2A cells also experience E2-induced apoptosis but evade dramatic cell death until approximately 14 days of treatment. To discover and define the mechanisms by which MCF-7:2A cells survive two weeks of E2 treatment, systematic experiments were performed in this study. The data suggest that MCF-7:2A cells employ stronger antioxidant defense mechanisms than do MCF-7:5C cells, and that oxidative stress is ultimately required for MCF-7:2A cells to die in response to E2 treatment. Tumor necrosis factor (TNF) family member activation is also essential for E2-induced apoptosis to occur in MCF-7:2A cells; upregulation of TNFα occurs simultaneously with oxidative stress activation. Although the unfolded protein response (UPR) signaling pattern is similar to that in MCF-7:5C cells, it is not sufficient to cause cell death in MCF-7:2A cells. Additionally, increased insulin-like growth factor receptor β (IGF-1Rβ) confers a mechanism of growth and anti-apoptotic advantage in MCF-7:2A cells.
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Copy and paste a formatted citation
Spandidos Publications style
Sweeney EE, Fan P and Jordan VC: Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells. Int J Oncol 44: 1529-1538, 2014.
APA
Sweeney, E.E., Fan, P., & Jordan, V.C. (2014). Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells. International Journal of Oncology, 44, 1529-1538. https://doi.org/10.3892/ijo.2014.2329
MLA
Sweeney, E. E., Fan, P., Jordan, V. C."Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells". International Journal of Oncology 44.5 (2014): 1529-1538.
Chicago
Sweeney, E. E., Fan, P., Jordan, V. C."Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells". International Journal of Oncology 44, no. 5 (2014): 1529-1538. https://doi.org/10.3892/ijo.2014.2329
Copy and paste a formatted citation
x
Spandidos Publications style
Sweeney EE, Fan P and Jordan VC: Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells. Int J Oncol 44: 1529-1538, 2014.
APA
Sweeney, E.E., Fan, P., & Jordan, V.C. (2014). Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells. International Journal of Oncology, 44, 1529-1538. https://doi.org/10.3892/ijo.2014.2329
MLA
Sweeney, E. E., Fan, P., Jordan, V. C."Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells". International Journal of Oncology 44.5 (2014): 1529-1538.
Chicago
Sweeney, E. E., Fan, P., Jordan, V. C."Mechanisms underlying differential response to estrogen-induced apoptosis in long-term estrogen-deprived breast cancer cells". International Journal of Oncology 44, no. 5 (2014): 1529-1538. https://doi.org/10.3892/ijo.2014.2329
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