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The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer

  • Authors:
    • Tian-Bao Wang
    • Bao-Guang Hu
    • Da-Wei Liu
    • Han-Ping Shi
    • Wen-Guang Dong
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, P.R. China, Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1861-1869
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    Published online on: March 19, 2014
       https://doi.org/10.3892/ijo.2014.2348
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Abstract

The aim of this study was to construct a lentiviral vector of CXCR4-siRNA (Lenti-CXCR4-siRNA) and investigate whether the vector can inhibit the growth, migration, invasion and hepatic metastasis of colorectal cancer (CRC). RT-PCR and western blotting were employed to identify the ideal RNA interference sequence. Lenti-CXCR4-siRNA was constructed and transfected into the SW480 cell line. We used RT-PCR and western blotting to measure the expression of CXCR4 RNA and protein, respectively; the MTS assay to assess the proliferation of SW480 cells; transwell chambers to estimate the inhibitory effect on migration and invasion; and the Balb/c nude mouse model of CRC to examine the inhibition of hepatic metastasis. The relative expression of the CXCR4 gene and protein was 5.4 and 18.95%, respectively, in the siCXCR4 group. The genes in the expression plasmid pLenti-CXCR4-siRNA were in the correct order. In the SW480, nonsense control (NC) and the Lenti-CXCR4-siRNA groups CXCR4 RNA levels were, respectively, 0.54±0.06, 1.00±0.03 and 0.11±0.04 (P=0.0001); CXCR4 protein levels were 0.60±0.03, 0.72±0.03 and 0.18±0.02 (P=0.0001); the OD value was 1.38±0.04 (P=0.0050), 1.28±0.05 (P=0.0256) and 0.92±0.06; SW480 cell number in migration test was 32±6.85, 32.63±1.69 and 0.75±0.71 (P=0.0000); SW480 cell number in the invasion test was 29.13±10.3, 30.38±6.09 and 0.63±0.74 (P=0.0000); hepatic metastasis number was 7.10±3.98 (P=0.034), 7.50±4.09 (P=0.019) and (3.50±2.51); hepatic metastasis mean weight (in g) was 2.25±2.51 (P=0.000), 2.11±2.38 (P=0.000) and 1.45±2.07. Lenti-CXCR4-siRNA constructs were correctly constructed and effectively inhibit the expression of CXCR4 RNA and protein, reducing the proliferation, migration, invasion capacity of SW480 cells and hepatic metastasis of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang T, Hu B, Liu D, Shi H and Dong W: The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer. Int J Oncol 44: 1861-1869, 2014.
APA
Wang, T., Hu, B., Liu, D., Shi, H., & Dong, W. (2014). The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer. International Journal of Oncology, 44, 1861-1869. https://doi.org/10.3892/ijo.2014.2348
MLA
Wang, T., Hu, B., Liu, D., Shi, H., Dong, W."The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer". International Journal of Oncology 44.6 (2014): 1861-1869.
Chicago
Wang, T., Hu, B., Liu, D., Shi, H., Dong, W."The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer". International Journal of Oncology 44, no. 6 (2014): 1861-1869. https://doi.org/10.3892/ijo.2014.2348
Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Hu B, Liu D, Shi H and Dong W: The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer. Int J Oncol 44: 1861-1869, 2014.
APA
Wang, T., Hu, B., Liu, D., Shi, H., & Dong, W. (2014). The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer. International Journal of Oncology, 44, 1861-1869. https://doi.org/10.3892/ijo.2014.2348
MLA
Wang, T., Hu, B., Liu, D., Shi, H., Dong, W."The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer". International Journal of Oncology 44.6 (2014): 1861-1869.
Chicago
Wang, T., Hu, B., Liu, D., Shi, H., Dong, W."The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer". International Journal of Oncology 44, no. 6 (2014): 1861-1869. https://doi.org/10.3892/ijo.2014.2348
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