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Article Open Access

A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer

  • Authors:
    • Reza Riahi
    • Priyadarshini Gogoi
    • Saedeh Sepehri
    • Yi Zhou
    • Kalyan Handique
    • Jim Godsey
    • Yixin Wang
  • View Affiliations / Copyright

    Affiliations: Ventana Medical Systems, Inc., A Member of the Roche Group, Tucson, AZ 85755, USA, DeNovo Sciences, Plymouth, MI 48170, USA
    Copyright: © Riahi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1870-1878
    |
    Published online on: March 21, 2014
       https://doi.org/10.3892/ijo.2014.2353
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Abstract

Circulating tumor cells (CTCs) have been shown in many studies as a possible biomarker for metastasis and may be instrumental for the spread of the disease. Despite advances in CTC capturing technologies, the low frequency of CTCs in cancer patients and the heterogeneity of the CTCs have limited the wide application of the technology in clinic. In this study, we investigated a novel microfluidic technology that uses a size- and deformability-based capture system to characterize CTCs. This unique platform not only allows flexibility in the selection of antibody markers but also segregates the CTCs in their own chambers, thus, enabling morphological, immunological and genetic characterization of each CTC at the single cell level. In this study, different breast cancer cell lines including MCF7, MDA-MB-231 and SKBR3, as well as a panel of breast cancer biomarkers were used to test the device. The technology can capture a wide range of cells with high reproducibility. The capturing efficiency of the cells is greater than 80%. In addition, the background of leukocytes is minimized because individual cells are segregated in their own chambers. The device captured both epithelial cancer cells such as MCF7 and SKBR3 and mesenchymal cells such as MDA-MB-231. Immunostaining of the captured cells on the microchannel device suggests that a panel of breast cancer biomarkers can be used to further characterize differential expression of the captured cells.
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View References

1. 

Weigelt B, Peterse JL and van’t Veer LJ: Breast cancer metastasis: markers and models. Nature Rev Cancer. 5:591–602. 2005. View Article : Google Scholar : PubMed/NCBI

2. 

Maheswaran S and Haber DA: Circulating tumor cells: a window into cancer biology and metastasis. Curr Opin Genet Dev. 20:96–99. 2010. View Article : Google Scholar : PubMed/NCBI

3. 

Pantel K and Alix-Panabieres C: Circulating tumour cells in cancer patients: challenges and perspectives. Trends Mol Med. 16:398–406. 2010. View Article : Google Scholar : PubMed/NCBI

4. 

Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, Reuben JM, Doyle GV, Allard WJ, Terstappen LW and Hayes DF: Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med. 351:781–791. 2004. View Article : Google Scholar : PubMed/NCBI

5. 

Cristofanilli M, Hayes DF, Budd GT, Ellis MJ, Stopeck A, Reuben JM, Doyle GV, Matera J, Allard WJ, Miller MC, Fritsche HA, Hortobagyi GN and Terstappen LW: Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer. J Clin Oncol. 23:1420–1430. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Pantel K, Brakenhoff RH and Brandt B: Detection, clinical relevance and specific biological properties of disseminating cancer cells in breast cancer patients. Nature Rev Cancer. 8:329–340. 2008. View Article : Google Scholar : PubMed/NCBI

7. 

Punnoose EA, Atwal SK, Spoerke JM, Savage H, Pandita A, Yeh RF, Pirzkall A, Fine BM, Amler LC, Chen DS and Lackner MR: Molecular biomarker analyses using circulating tumor cells. PLoS One. 5:e125712010. View Article : Google Scholar

8. 

Kirby BJ, Jodari M, Loftus, Gakhar G, Pratt ED, Chanel-Vos C, Gleghorn JP, Santana SM, Liu H, Smith JP, Navarro VN, Tagawa ST, Bander NH, Nanus DM and Giannakakou P: Functional characterization of circulating tumor cells with a prostate-cancer-specific microfluidic device. PLoS One. 7:e359162012. View Article : Google Scholar

9. 

Riethdorf S, Fritsche H, Muller V, Rau T, Schindlbeck C, Rack B, Janni W, Coith C, Beck K, Jänicke F, Jackson S, Gornet T, Cristofanilli M and Pantel K: Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system. Clin Cancer Res. 13:920–928. 2007. View Article : Google Scholar : PubMed/NCBI

10. 

Reinholz MM, Nibbe A, Jonart LM, Kitzmann K, Suman VJ, Ingle JN, Houghton R, Zehentner B, Roche PC and Lingle WL: Evaluation of a panel of tumor markers for molecular detection of circulating cancer cells in women with suspected breast cancer. Clin Cancer Res. 11:3722–3732. 2005. View Article : Google Scholar : PubMed/NCBI

11. 

Warner EA, Kotz KT, Ungaro RF, Abouhamze AS, Lopez MC, Cuenca AG, Kelly-Scumpia KM, Moreno C, O’Malley KA, Lanz JD, Baker HV, Martin LC, Toner M, Tompkins RG, Efron PA and Moldawer LL: Microfluidics-based capture of human neutrophils for expression analysis in blood and bronchoalveolar lavage. Lab Invest. 91:1787–1795. 2011. View Article : Google Scholar : PubMed/NCBI

12. 

Dong Y, Skelley AM, Merdek KD, Sprott KM, Jiang C, Pierceall WE, Lin J, Stocum M, Carney WP and Smirnov DA: Microfluidics and circulating tumor cells. J Mol Diagn. 15:149–157. 2013. View Article : Google Scholar : PubMed/NCBI

13. 

Zhang Z and Nagrath S: Microfluidics and cancer: are we there yet? Biomed Microdevices. 15:595–609. 2013. View Article : Google Scholar : PubMed/NCBI

14. 

Nagrath S, Sequist LV, Maheswaran S, Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA and Toner M: Isolation of rare circulating tumor cells in cancer patients by microchip technology. Nature. 450:1235–1239. 2007. View Article : Google Scholar : PubMed/NCBI

15. 

Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP Jr, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA and Toner M: Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci USA. 107:18392–18397. 2010. View Article : Google Scholar : PubMed/NCBI

16. 

Li P, Stratton ZS, Dao M, Ritz J and Huang TJ: Probing circulating tumor cells in microfluidics. Lab Chip. 13:602–609. 2013. View Article : Google Scholar

17. 

Saliba AE, Saias L, Psychari E, Minc N, Simon D, Bidard FC, Mathiot C, Pierga JY, Fraisier V, Salamero J, Saada V, Farace F, Vielh P, Malaquin L and Viovy JL: Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays. Proc Natl Acad Sci USA. 107:14524–14529. 2010. View Article : Google Scholar : PubMed/NCBI

18. 

Warkiani ME, Guan G, Luan KB, Lee WC, Bhagat AA, Kant Chaudhuri P, Tan DS, Lim WT, Lee SC, Chen PC, Lim CT and Han J: Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells. Lab Chip. 14:128–137. 2013. View Article : Google Scholar : PubMed/NCBI

19. 

Ring AE, Zabaglo L, Ormerod MG, Smith IE and Dowsett M: Detection of circulating epithelial cells in the blood of patients with breast cancer: comparison of three techniques. Br J Cancer. 92:906–912. 2005. View Article : Google Scholar : PubMed/NCBI

20. 

Wicha MS and Hayes DF: Circulating tumor cells: not all detected cells are bad and not all bad cells are detected. J Clin Oncol. 29:1508–1511. 2011. View Article : Google Scholar : PubMed/NCBI

21. 

Smirnov DA, Zweitzig DR, Foulk BW, Miller MC, Doyle GV, Pienta KJ, Meropol NJ, Weiner LM, Cohen SJ, Moreno JG, Connelly MC, Terstappen LW and O’Hara SM: Global gene expression profiling of circulating tumor cells. Cancer Res. 65:4993–4997. 2005. View Article : Google Scholar : PubMed/NCBI

22. 

Sieuwerts AM, Mostert B, Bolt-de Vries J, Peeters D, de Jongh FE, Stouthard JM, Dirix LY, van Dam PA, Van Galen A, de Weerd V, Kraan J, van der Spoel P, Ramírez-Moreno R, van Deurzen CH, Smid M, Yu JX, Jiang J, Wang Y, Gratama JW, Sleijfer S, Foekens JA and Martens JW: mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients. Clin Cancer Res. 17:3600–3618. 2011. View Article : Google Scholar : PubMed/NCBI

23. 

Tang P, Skinner KA and Hicks DG: Molecular Classification of breast carcinomas by immunohistochemical analysis: are we ready? Diagn Mol Pathol. 18:125–132. 2009. View Article : Google Scholar : PubMed/NCBI

24. 

Subik K, Lee JF, Baxter L, Strzepek T, Costello D, Crowley P, Xing L, Hung MC, Bonfiglio T, Hicks DG and Tang P: The expression patterns of ER, PR, HER2, CK5/6, EGFR, Ki-67 and AR by immunohistochemical analysis in breast cancer cell lines. Breast Cancer. 4:35–41. 2010.PubMed/NCBI

25. 

Ignatiadis M, Kallergi G, Ntoulia M, Perraki M, Apostolaki S, Kafousi M, Chlouverakis G, Stathopoulos E, Lianidou E, Georgoulias V and Mavroudis D: Prognostic value of the molecular detection of circulating tumor cells using a multi-marker reverse transcription-PCR assay for cytokeratin 19, mammaglobin A, and HER2 in early breast cancer. Clin Cancer Res. 14:2593–2600. 2008. View Article : Google Scholar

26. 

US Patent Application # US 2013/0190212 A1 (DeNovo Sciences).

27. 

Allan AL and Keeney M: Circulating tumor cell analysis: technical and statistical considerations for application to the clinic. J Oncol. 426218:e1–e10. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Riahi R, Gogoi P, Sepehri S, Zhou Y, Handique K, Godsey J and Wang Y: A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer. Int J Oncol 44: 1870-1878, 2014.
APA
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., & Wang, Y. (2014). A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer. International Journal of Oncology, 44, 1870-1878. https://doi.org/10.3892/ijo.2014.2353
MLA
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., Wang, Y."A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer". International Journal of Oncology 44.6 (2014): 1870-1878.
Chicago
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., Wang, Y."A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer". International Journal of Oncology 44, no. 6 (2014): 1870-1878. https://doi.org/10.3892/ijo.2014.2353
Copy and paste a formatted citation
x
Spandidos Publications style
Riahi R, Gogoi P, Sepehri S, Zhou Y, Handique K, Godsey J and Wang Y: A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer. Int J Oncol 44: 1870-1878, 2014.
APA
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., & Wang, Y. (2014). A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer. International Journal of Oncology, 44, 1870-1878. https://doi.org/10.3892/ijo.2014.2353
MLA
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., Wang, Y."A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer". International Journal of Oncology 44.6 (2014): 1870-1878.
Chicago
Riahi, R., Gogoi, P., Sepehri, S., Zhou, Y., Handique, K., Godsey, J., Wang, Y."A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer". International Journal of Oncology 44, no. 6 (2014): 1870-1878. https://doi.org/10.3892/ijo.2014.2353
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