Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression

  • Authors:
    • Ga Bin Park
    • Daejin Kim
    • Hoi Soo Yoon
    • Yeong-Seok Kim
    • Hyun-Kyung Lee
    • Ki Tae Kim
    • Dae Hoon Jeong
    • Dae Young Hur
  • View Affiliations

  • Published online on: March 27, 2014     https://doi.org/10.3892/ijo.2014.2361
  • Pages: 2016-2024
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Abstract

Centrocyte/centroblast marker 1 (CM1) has been identified as a pro-apoptosis molecule on B-cell lymphoma cells as well as several types of cancer cells. In this study, we investigated its signaling mechanism in HeLa cells after treatment with cisplatin in order to potentially identify a new therapeutic target. The CM1 molecule was induced on the surface of cisplatin-exposed HeLa cells. In these cells, ligation of CM1 with anti-CM1 monoclonal antibodies inhibited cell proliferation and produced reactive oxygen species. Fas ligand (FasL) expression was upregulated without upregulating Fas in cisplatin-exposed HeLa cells after CM1 stimulation. Pretreatment with N-acetylcysteine, a pan-capase inhibitor, and ZB4, an antagonistic anti-Fas antibody, effectively inhibited the apoptotic effect triggered by CM1. CM1 ligation induced apoptosis through disruption of the mitochondrial membrane potential, decreased Bcl-2 and phosphorylated ERK expression. These findings identify CM1 as a potential new therapeutic target related to cisplatin-exposed cervical cancer.
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June-2014
Volume 44 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park GB, Kim D, Yoon HS, Kim Y, Lee H, Kim KT, Jeong DH and Hur DY: Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression. Int J Oncol 44: 2016-2024, 2014.
APA
Park, G.B., Kim, D., Yoon, H.S., Kim, Y., Lee, H., Kim, K.T. ... Hur, D.Y. (2014). Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression. International Journal of Oncology, 44, 2016-2024. https://doi.org/10.3892/ijo.2014.2361
MLA
Park, G. B., Kim, D., Yoon, H. S., Kim, Y., Lee, H., Kim, K. T., Jeong, D. H., Hur, D. Y."Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression". International Journal of Oncology 44.6 (2014): 2016-2024.
Chicago
Park, G. B., Kim, D., Yoon, H. S., Kim, Y., Lee, H., Kim, K. T., Jeong, D. H., Hur, D. Y."Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression". International Journal of Oncology 44, no. 6 (2014): 2016-2024. https://doi.org/10.3892/ijo.2014.2361