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International Journal of Oncology
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Article

Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence

  • Authors:
    • Lijuan Wang
    • Suxia Han
    • Guihua Jin
    • Xia Zhou
    • Meng Li
    • Xia Ying
    • Le Wang
    • Huili Wu
    • Qing Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China
  • Pages: 2041-2049
    |
    Published online on: April 2, 2014
       https://doi.org/10.3892/ijo.2014.2363
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Abstract

Whole genome transcriptomic analyses have identified a large number of long non-coding RNAs (lncRNAs), many of which are involved in a variety of biological functions. However, their functions and molecular mechanisms associated with prostate cancer (PCa) progression to a virulent and androgen-independent (AI) form remain elusive. Herein, we investigated the lncRNA expression profiles of the indolent, androgen-dependent (AD) LNCaP cell line to the aggressive metastatic, AI C4-2 cell line using microarray technology. The differentially expressed lncRNAs and genes were identified by microarray technology and the association in cis or in trans was analyzed to find potential lncRNA target genes. Expression of candidate lncRNAs and putative targets was evaluated by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The functions of linc00963 on cell proliferation, apoptosis, migration and invasion were evaluated by a knockdown strategy in vitro using MTT, flow cytometric analysis and transwell chamber assays. lncRNAs (n=134) were differentially expressed (FDR <0.001 and fold change ≥2) between the LNCaP and C4-2 cell lines. Linc00963 was upregulated most obviously evaluated by qRT-PCR. Knockdown of linc00963 attenuated C4-2 cell proliferation, motility, invasion ability, the expression of EGFR and phosphorylation levels of AKT, and promoted cell apoptosis. Linc00963 was involved in the prostate cancer transition from androgen-dependent to androgen-independent and metastasis via the EGFR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Han S, Jin G, Zhou X, Li M, Ying X, Wang L, Wu H and Zhu Q: Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence . Int J Oncol 44: 2041-2049, 2014.
APA
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X. ... Zhu, Q. (2014). Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence . International Journal of Oncology, 44, 2041-2049. https://doi.org/10.3892/ijo.2014.2363
MLA
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X., Wang, L., Wu, H., Zhu, Q."Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence ". International Journal of Oncology 44.6 (2014): 2041-2049.
Chicago
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X., Wang, L., Wu, H., Zhu, Q."Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence ". International Journal of Oncology 44, no. 6 (2014): 2041-2049. https://doi.org/10.3892/ijo.2014.2363
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Han S, Jin G, Zhou X, Li M, Ying X, Wang L, Wu H and Zhu Q: Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence . Int J Oncol 44: 2041-2049, 2014.
APA
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X. ... Zhu, Q. (2014). Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence . International Journal of Oncology, 44, 2041-2049. https://doi.org/10.3892/ijo.2014.2363
MLA
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X., Wang, L., Wu, H., Zhu, Q."Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence ". International Journal of Oncology 44.6 (2014): 2041-2049.
Chicago
Wang, L., Han, S., Jin, G., Zhou, X., Li, M., Ying, X., Wang, L., Wu, H., Zhu, Q."Linc00963: A novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence ". International Journal of Oncology 44, no. 6 (2014): 2041-2049. https://doi.org/10.3892/ijo.2014.2363
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