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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR

  • Authors:
    • Hong Zhu
    • Maria Pik Wong
    • Vicky Tin
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, Department of Pathology, The University of Hong Kong, Hong Kong, SAR, P.R. China
  • Pages: 2068-2076
    |
    Published online on: April 11, 2014
       https://doi.org/10.3892/ijo.2014.2384
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Abstract

Genomic abnormalities are the hallmark of cancers and may harbor potential candidate genes important for cancer development and progression. We performed array comparative genomic hybridization (array CGH) on 36 cases of primary lung adenocarcinoma (AD) using an array containing 2621 BAC or PAC clones spanning the genome at an average interval of 1 Mb. Array CGH identified the commonest aberrations consisting of DNA gains within 1p, 1q, 5p, 5q, 7p, 7q, 8q, 11q, 12p, 13q, 16p, 17q, 20q, and losses with 6q, 9p, 10q and 18q. High-level copy gains involved mainly 7p21-p15 and 20q13.3. Dual color fluorescence in situ hybridization (FISH) was performed on a selective locus for validation of array CGH results. Genomic aberrations were compared with different clinicopathological features and a trend of higher number of aberrations in tumors with aggressive phenotypes and current tobacco exposure was identified. According to array CGH data, 23 candidate genes were selected for quantitative PCR (qPCR) analysis. The concordance observed between the genomic and expression changes in most of the genes suggested that they could be candidate cancer-related genes that contributed to the development of lung AD.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Wong MP and Tin V: High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR. Int J Oncol 44: 2068-2076, 2014.
APA
Zhu, H., Wong, M.P., & Tin, V. (2014). High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR. International Journal of Oncology, 44, 2068-2076. https://doi.org/10.3892/ijo.2014.2384
MLA
Zhu, H., Wong, M. P., Tin, V."High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR". International Journal of Oncology 44.6 (2014): 2068-2076.
Chicago
Zhu, H., Wong, M. P., Tin, V."High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR". International Journal of Oncology 44, no. 6 (2014): 2068-2076. https://doi.org/10.3892/ijo.2014.2384
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Wong MP and Tin V: High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR. Int J Oncol 44: 2068-2076, 2014.
APA
Zhu, H., Wong, M.P., & Tin, V. (2014). High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR. International Journal of Oncology, 44, 2068-2076. https://doi.org/10.3892/ijo.2014.2384
MLA
Zhu, H., Wong, M. P., Tin, V."High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR". International Journal of Oncology 44.6 (2014): 2068-2076.
Chicago
Zhu, H., Wong, M. P., Tin, V."High-resolution detection of recurrent aberrations in lung adenocarcinomas by array comparative genomic hybridization and expression analysis of selective genes by quantitative PCR". International Journal of Oncology 44, no. 6 (2014): 2068-2076. https://doi.org/10.3892/ijo.2014.2384
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