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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma

  • Authors:
    • Jin-Wei Miao
    • Li-Jiang Liu
    • Jie Huang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China, Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan 430056, P.R. China
  • Pages: 165-176
    |
    Published online on: May 6, 2014
       https://doi.org/10.3892/ijo.2014.2422
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Abstract

Epithelial-mesenchymal transition (EMT) is an important process in the invasion and metastasis of human cervical carcinoma. The pro-inflammatory cytokine interleukin-6 (IL-6) has been shown as an EMT inducer in multiple carcinomas. However, whether the EMT program can be induced by IL-6 and the mechanisms underlying the IL-6-induced EMT in human cervical carcinoma remain to be determined. In this study, we show that IL-6 receptor (IL-6R) and signal transducer and activator of transcription 3 (Stat3) were highly expressed in human cervical squamous cell carcinoma (CSCC) tissues, and the expression of EMT markers was reversed in well-differentiated and poorly-differentiated human CSCC. Additional experiments showed that IL-6 exposure in cervical carcinoma cell lines induced IL-6R and Stat3 expression, markedly promoted cell growth, and altered cell morphology. The treatment of cervical carcinoma cell lines with IL-6 resulted in downregulation of E-Cadherin and upregulation of Vimentin. Importantly, knockdown of Stat3 significantly reversed the IL-6-induced EMT program, suggesting that Stat3 is necessary for IL-6-induced EMT in the progression of human cervical carcinoma. Moreover, Slug, a member of the Snail family of EMT regulators, was observed to be associated with the expression of Stat3. We concluded that IL-6 plays an important role through Stat3 in the EMT induction and can be a potential therapeutic target and biomarker for human cervical carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Miao J, Liu L and Huang J: Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma. Int J Oncol 45: 165-176, 2014.
APA
Miao, J., Liu, L., & Huang, J. (2014). Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma. International Journal of Oncology, 45, 165-176. https://doi.org/10.3892/ijo.2014.2422
MLA
Miao, J., Liu, L., Huang, J."Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma". International Journal of Oncology 45.1 (2014): 165-176.
Chicago
Miao, J., Liu, L., Huang, J."Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma". International Journal of Oncology 45, no. 1 (2014): 165-176. https://doi.org/10.3892/ijo.2014.2422
Copy and paste a formatted citation
x
Spandidos Publications style
Miao J, Liu L and Huang J: Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma. Int J Oncol 45: 165-176, 2014.
APA
Miao, J., Liu, L., & Huang, J. (2014). Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma. International Journal of Oncology, 45, 165-176. https://doi.org/10.3892/ijo.2014.2422
MLA
Miao, J., Liu, L., Huang, J."Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma". International Journal of Oncology 45.1 (2014): 165-176.
Chicago
Miao, J., Liu, L., Huang, J."Interleukin-6-induced epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in human cervical carcinoma". International Journal of Oncology 45, no. 1 (2014): 165-176. https://doi.org/10.3892/ijo.2014.2422
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