Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids

  • Authors:
    • Ken Ohnishi
    • Toshiaki Tani
    • Shin-Ichi Bando
    • Nobuo Kubota
    • Yoshihiro Fujii
    • Osamu Hatano
    • Hirosi Harada
  • View Affiliations

  • Published online on: June 3, 2014     https://doi.org/10.3892/ijo.2014.2483
  • Pages: 581-586
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Abstract

Recent studies showed that the stemness of cancer stem cells is maintained under a hypoxic microenvironment. However, the relationship of the hypoxic microenvironment in a three-dimensional cell mass and the induction of cancer stem cell-like phenotype is not well known. We examined the relationship between CD133 expression and the hypoxic microenvironment using glioblastoma spheroids formed with the T98G cell line. CD133AC133- and HIF-1α-positive cells were observed in the marginal region of the central hypoxic area positive for HIF-1α 10 days after plating T98G cells. CD133AC133-positive cells were positive for nestin. Quantitative PCR analysis showed that the CD133 expression level is not different in spheroids during the tested period after spheroid formation, indicating that post-translational regulation of the CD133 protein mediates positivity to CD133AC133. When spheroids were trypsinized and the dissociated cells were cultured under the adherent monolayer conditions, the CD133AC133-positive cells gradually disappeared. These results show that CD133AC133-positive cells, which may incline toward undifferentiated cells because of nestin positivity, are plastically induced under the different culture conditions, spheroid and monolayer. In this plasticity, HIF-1α is involved in the induction and maintenance of CD133AC133-positive cells. Spheroids as an in vitro tumor model are useful to study the dynamic changes in the tumor cell phenotype in the different cell microenvironments.
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August-2014
Volume 45 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., & Harada, H. (2014). Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International Journal of Oncology, 45, 581-586. https://doi.org/10.3892/ijo.2014.2483
MLA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45.2 (2014): 581-586.
Chicago
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45, no. 2 (2014): 581-586. https://doi.org/10.3892/ijo.2014.2483