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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August-2014 Volume 45 Issue 2

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids

  • Authors:
    • Ken Ohnishi
    • Toshiaki Tani
    • Shin-Ichi Bando
    • Nobuo Kubota
    • Yoshihiro Fujii
    • Osamu Hatano
    • Hirosi Harada
  • View Affiliations / Copyright

    Affiliations: Department of Biology, Center for Humanity and Sciences, Ibaraki Prefectural University of Health Sciences, Inashiki-gun, Ibaraki 300-0394, Japan, Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, Inashiki-gun, Ibaraki 300-0394, Japan, Department of Anatomy, Nara Medical University, Kashihara, Nara 634-8521, Japan, Group of Radiation and Tumor Biology, Career-Path Promotion Unit for Young Life Scientists, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku 606-8501, Japan
  • Pages: 581-586
    |
    Published online on: June 3, 2014
       https://doi.org/10.3892/ijo.2014.2483
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Abstract

Recent studies showed that the stemness of cancer stem cells is maintained under a hypoxic microenvironment. However, the relationship of the hypoxic microenvironment in a three-dimensional cell mass and the induction of cancer stem cell-like phenotype is not well known. We examined the relationship between CD133 expression and the hypoxic microenvironment using glioblastoma spheroids formed with the T98G cell line. CD133AC133- and HIF-1α-positive cells were observed in the marginal region of the central hypoxic area positive for HIF-1α 10 days after plating T98G cells. CD133AC133-positive cells were positive for nestin. Quantitative PCR analysis showed that the CD133 expression level is not different in spheroids during the tested period after spheroid formation, indicating that post-translational regulation of the CD133 protein mediates positivity to CD133AC133. When spheroids were trypsinized and the dissociated cells were cultured under the adherent monolayer conditions, the CD133AC133-positive cells gradually disappeared. These results show that CD133AC133-positive cells, which may incline toward undifferentiated cells because of nestin positivity, are plastically induced under the different culture conditions, spheroid and monolayer. In this plasticity, HIF-1α is involved in the induction and maintenance of CD133AC133-positive cells. Spheroids as an in vitro tumor model are useful to study the dynamic changes in the tumor cell phenotype in the different cell microenvironments.
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Copy and paste a formatted citation
Spandidos Publications style
Ohnishi K, Tani T, Bando S, Kubota N, Fujii Y, Hatano O and Harada H: Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. Int J Oncol 45: 581-586, 2014.
APA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., & Harada, H. (2014). Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International Journal of Oncology, 45, 581-586. https://doi.org/10.3892/ijo.2014.2483
MLA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45.2 (2014): 581-586.
Chicago
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45, no. 2 (2014): 581-586. https://doi.org/10.3892/ijo.2014.2483
Copy and paste a formatted citation
x
Spandidos Publications style
Ohnishi K, Tani T, Bando S, Kubota N, Fujii Y, Hatano O and Harada H: Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. Int J Oncol 45: 581-586, 2014.
APA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., & Harada, H. (2014). Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International Journal of Oncology, 45, 581-586. https://doi.org/10.3892/ijo.2014.2483
MLA
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45.2 (2014): 581-586.
Chicago
Ohnishi, K., Tani, T., Bando, S., Kubota, N., Fujii, Y., Hatano, O., Harada, H."Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids". International Journal of Oncology 45, no. 2 (2014): 581-586. https://doi.org/10.3892/ijo.2014.2483
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