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Article

Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer

  • Authors:
    • Yoshifumi Umemori
    • Kageaki Kuribayashi
    • Shinya Nirasawa
    • Takashi Kondoh
    • Maki Tanaka
    • Daisuke Kobayashi
    • Naoki Watanabe
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan
  • Pages: 1043-1050
    |
    Published online on: June 10, 2014
       https://doi.org/10.3892/ijo.2014.2489
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Abstract

The phosphatidylinositol 3-kinase pathway transduces cell survival signals in different malignancies. Protein kinase C ζ (PKCζ) is one of the molecules involved in this pathway. In this study, we investigated the role of PKCζ in apoptosis. Short interfering RNA against PKCζ (siPKCζ) sensitized HCT116 and SW480 colon cancer cells to TRAIL‑induced apoptosis. Among anti-apoptotic proteins, survivin protein and mRNA expression levels decreased after siPKCζ transfection while protein half-life did not change. The expression levels of survivin and PKCζ were correlated in 18 colon cancer specimens (r=0.72, P=3.01x10‑4). Chemosensitivity to 5-FU was enhanced by siPKCζ in HCT116 and SW480 cells. These results indicate that PKCζ regulates survivin expression levels and inhibits apoptosis in colon cancer cells. This study provides a rationale for targeting PKCζ in combination with chemotherapy for colon cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D and Watanabe N: Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer. Int J Oncol 45: 1043-1050, 2014.
APA
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., & Watanabe, N. (2014). Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer. International Journal of Oncology, 45, 1043-1050. https://doi.org/10.3892/ijo.2014.2489
MLA
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., Watanabe, N."Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer". International Journal of Oncology 45.3 (2014): 1043-1050.
Chicago
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., Watanabe, N."Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer". International Journal of Oncology 45, no. 3 (2014): 1043-1050. https://doi.org/10.3892/ijo.2014.2489
Copy and paste a formatted citation
x
Spandidos Publications style
Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D and Watanabe N: Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer. Int J Oncol 45: 1043-1050, 2014.
APA
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., & Watanabe, N. (2014). Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer. International Journal of Oncology, 45, 1043-1050. https://doi.org/10.3892/ijo.2014.2489
MLA
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., Watanabe, N."Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer". International Journal of Oncology 45.3 (2014): 1043-1050.
Chicago
Umemori, Y., Kuribayashi, K., Nirasawa, S., Kondoh, T., Tanaka, M., Kobayashi, D., Watanabe, N."Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer". International Journal of Oncology 45, no. 3 (2014): 1043-1050. https://doi.org/10.3892/ijo.2014.2489
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