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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November-2014 Volume 45 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma

  • Authors:
    • Noriyuki Tanaka
    • Hiroshi Yoshida
    • Yoshio Suzuki
    • Kenichi Harigaya
  • View Affiliations / Copyright

    Affiliations: Department of Molecular and Tumor Pathology, Chiba University Graduate School of Medicine, Chuo-ku, Chiba 260-8670, Japan, Department of Pathology, Asahi General Hospital, Chiba 289-2515, Japan
  • Pages: 1921-1928
    |
    Published online on: August 8, 2014
       https://doi.org/10.3892/ijo.2014.2591
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Abstract

Alternative splicing provides additional genomic complexity by producing multiple mRNAs and protein variants from any given gene. Splice variants have been identified in a large variety of cancer genes, suggesting that widespread aberrant and alternative splicing may be a consequence or even a cause of cancer. Human ortholog of mammalian enabled (hMena), a family of enabled/vasodilator-stimulated phosphoproteins (Ena/VASP), is an actin regulatory protein involved in the regulation of cell motility. hMena has been shown to have several splice variants, including the hMenaINV isoform, expressed in invasive cancer cells, and the epithelial-specific isoform, hMena11a. We assessed the relative mRNA expression of hMena splice variants in 50 cases of invasive ductal breast carcinoma of no special type (IDC-NST) and 45 cases of ductal breast carcinoma in situ (DCIS) with special reference to non-neoplastic breast epithelial tissues. The samples were dissected from their respective regions by laser microdissection. Our results confirmed previous reports that hMenaINV expression is augmented during tumor progression, while hMena11a is downregulated. Furthermore, simultaneous expression of hMena11a and hMenaINV was found only in malignant lesions, while their expression was hardly detected in normal breast tissue and benign proliferative breast lesions. These results indicate that the higher relative expression of hMena11a compared with hMenaINV may predict malignant transformation in breast epithelial cells, and, furthermore, a reversal of expression of hMena11a and hMenaINV may dictate the state of cancer progression. Here, we demonstrate that determination of hMena11a and hMenaINV expression could be a useful biomarker for predicting malignant behavior in breast epithelial lesions, and show that their relative expression is linked to adverse prognostic factors. Although the biological activity of the majority of alternatively spliced isoforms and their contribution to cancer biology has yet to be determined, their elucidation will have a large impact on therapeutic strategies for cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tanaka N, Yoshida H, Suzuki Y and Harigaya K: Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma. Int J Oncol 45: 1921-1928, 2014.
APA
Tanaka, N., Yoshida, H., Suzuki, Y., & Harigaya, K. (2014). Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma. International Journal of Oncology, 45, 1921-1928. https://doi.org/10.3892/ijo.2014.2591
MLA
Tanaka, N., Yoshida, H., Suzuki, Y., Harigaya, K."Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma". International Journal of Oncology 45.5 (2014): 1921-1928.
Chicago
Tanaka, N., Yoshida, H., Suzuki, Y., Harigaya, K."Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma". International Journal of Oncology 45, no. 5 (2014): 1921-1928. https://doi.org/10.3892/ijo.2014.2591
Copy and paste a formatted citation
x
Spandidos Publications style
Tanaka N, Yoshida H, Suzuki Y and Harigaya K: Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma. Int J Oncol 45: 1921-1928, 2014.
APA
Tanaka, N., Yoshida, H., Suzuki, Y., & Harigaya, K. (2014). Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma. International Journal of Oncology, 45, 1921-1928. https://doi.org/10.3892/ijo.2014.2591
MLA
Tanaka, N., Yoshida, H., Suzuki, Y., Harigaya, K."Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma". International Journal of Oncology 45.5 (2014): 1921-1928.
Chicago
Tanaka, N., Yoshida, H., Suzuki, Y., Harigaya, K."Relative expression of hMena11a and hMenaINV splice isoforms is a useful biomarker in development and progression of human breast carcinoma". International Journal of Oncology 45, no. 5 (2014): 1921-1928. https://doi.org/10.3892/ijo.2014.2591
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