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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis

  • Authors:
    • Binil Eldhose
    • Mia Gunawan
    • Mahbubur Rahman
    • Mukalel S. Latha
    • Vicente Notario
  • View Affiliations / Copyright

    Affiliations: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala-686560, India, Department of Radiation Medicine, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
  • Pages: 1913-1920
    |
    Published online on: August 8, 2014
       https://doi.org/10.3892/ijo.2014.2592
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Abstract

Despite increased use of early detection methods and more aggressive treatment strategies, the worldwide incidence of colorectal cancer is still on the rise. Consequently, it remains urgent to identify novel agents with enhanced efficacy in prevention and/or therapeutic protocols. Our studies focused on the use of Plumbagin, a natural phytochemical that showed promising results against other tumor types, to determine its effectiveness in blocking the proliferation and survival of colon cancer cells in experimental protocols mimicking the environment in primary tumors (attached culture conditions) and in circulating tumor cells (unattached conditions). Under both experimental settings, exposure of HCT116 cells to Plumbagin concentrations in the low micromolar range resulted in cell cycle arrest at the G1 phase, apoptosis via the mitochondrial cell death pathway, and increased production of reactive oxygen species. The cell cycle effects were more noticeable in attached cells, whereas the induction of cell death was more evident in unattached cells. These effects were consistent with the nature and the magnitude of the alterations induced by Plumbagin on the expression levels of a set of proteins known to play key roles in the regulation of cell cycle dynamics, apoptosis mechanisms and cell proliferation. In light of its previously reported lack of toxicity on normal colon cells and the striking anti-survival effect on colon cancer cells observed in our study, Plumbagin should be considered a promising drug for the treatment of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Eldhose B, Gunawan M, Rahman M, Latha MS and Notario V: Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis. Int J Oncol 45: 1913-1920, 2014.
APA
Eldhose, B., Gunawan, M., Rahman, M., Latha, M.S., & Notario, V. (2014). Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis. International Journal of Oncology, 45, 1913-1920. https://doi.org/10.3892/ijo.2014.2592
MLA
Eldhose, B., Gunawan, M., Rahman, M., Latha, M. S., Notario, V."Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis". International Journal of Oncology 45.5 (2014): 1913-1920.
Chicago
Eldhose, B., Gunawan, M., Rahman, M., Latha, M. S., Notario, V."Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis". International Journal of Oncology 45, no. 5 (2014): 1913-1920. https://doi.org/10.3892/ijo.2014.2592
Copy and paste a formatted citation
x
Spandidos Publications style
Eldhose B, Gunawan M, Rahman M, Latha MS and Notario V: Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis. Int J Oncol 45: 1913-1920, 2014.
APA
Eldhose, B., Gunawan, M., Rahman, M., Latha, M.S., & Notario, V. (2014). Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis. International Journal of Oncology, 45, 1913-1920. https://doi.org/10.3892/ijo.2014.2592
MLA
Eldhose, B., Gunawan, M., Rahman, M., Latha, M. S., Notario, V."Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis". International Journal of Oncology 45.5 (2014): 1913-1920.
Chicago
Eldhose, B., Gunawan, M., Rahman, M., Latha, M. S., Notario, V."Plumbagin reduces human colon cancer cell survival by inducing cell cycle arrest and mitochondria-mediated apoptosis". International Journal of Oncology 45, no. 5 (2014): 1913-1920. https://doi.org/10.3892/ijo.2014.2592
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