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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors

  • Authors:
    • Aeyung Kim
    • Minju Im
    • Jin Yeul Ma
  • View Affiliations / Copyright

    Affiliations: Korean Medicine (KM)-Based Herbal Drug Development Center, Korea Institute of Oriental Medicine (KIOM), Daejeon 305-811, Republic of Korea
  • Pages: 1937-1948
    |
    Published online on: August 18, 2014
       https://doi.org/10.3892/ijo.2014.2606
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Abstract

Anisi stellati fructus (ASF), commonly known as star anise, has long been used as a traditional Chinese medicine to treat inflammation, nervousness, insomnia and pain. In recent studies, it has been demonstrated that ASF possesses anti-bacterial, anti-fungal and anti-oxidant activities, as well as exhibits inhibitory effects on capillary‑like tube formation in human umbilical vein endothelial cells (HUVECs). However, the effects of ASF extract on the metastatic potential of malignant tumor cells have not been examined. In this study, we found that daily oral administration of ASF (50 mg/kg) remarkably reduced the number of pulmonary metastatic colonies of B16F10 cells in C57BL/6J mice with no observed systemic toxicity. In an in vitro system, ASF inhibited metastatic properties, including anchorage‑independent colony formation, migration and invasion. Upon phorbol 12-myristate 13-acetate (PMA) stimulation, the mRNA levels of matrix metalloproteinases (MMPs) -9, -13, -14 and urokinase plasminogen activator (uPA) decreased in a dose-dependent manner with ASF treatment. Gelatinase, type I collagenase, and uPA activities were also suppressed efficiently by ASF treatment. In response to PMA, NF-κB and AP-1 activation as well as p38 phosphorylation, which are crucial for MMP activation, were significantly decreased by ASF. In particular, ASF considerably inhibited tumor-induced HUVEC migration and tube formation and suppressed in vivo tumor-induced angiogenesis via a reduction of pro-angiogenic factors in tumors. These results collectively indicate that ASF might be useful in the management of metastatic malignant tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Kim A, Im M and Ma JY: Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors. Int J Oncol 45: 1937-1948, 2014.
APA
Kim, A., Im, M., & Ma, J.Y. (2014). Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors. International Journal of Oncology, 45, 1937-1948. https://doi.org/10.3892/ijo.2014.2606
MLA
Kim, A., Im, M., Ma, J. Y."Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors". International Journal of Oncology 45.5 (2014): 1937-1948.
Chicago
Kim, A., Im, M., Ma, J. Y."Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors". International Journal of Oncology 45, no. 5 (2014): 1937-1948. https://doi.org/10.3892/ijo.2014.2606
Copy and paste a formatted citation
x
Spandidos Publications style
Kim A, Im M and Ma JY: Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors. Int J Oncol 45: 1937-1948, 2014.
APA
Kim, A., Im, M., & Ma, J.Y. (2014). Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors. International Journal of Oncology, 45, 1937-1948. https://doi.org/10.3892/ijo.2014.2606
MLA
Kim, A., Im, M., Ma, J. Y."Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors". International Journal of Oncology 45.5 (2014): 1937-1948.
Chicago
Kim, A., Im, M., Ma, J. Y."Anisi stellati fructus extract attenuates the in vitro and in vivo metastatic and angiogenic potential of malignant cancer cells by downregulating proteolytic activity and pro-angiogenic factors". International Journal of Oncology 45, no. 5 (2014): 1937-1948. https://doi.org/10.3892/ijo.2014.2606
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