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Article

Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines

  • Authors:
    • Wenqing Liang
    • Bo Gao
    • Guojian Xu
    • Dong Weng
    • Minghua Xie
    • Yu Qian
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, P.R. China, Department of Orthopedics, The 306th Hospital of PLA, Beijing 100101, P.R. China
  • Pages: 2475-2485
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    Published online on: September 22, 2014
       https://doi.org/10.3892/ijo.2014.2664
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Abstract

Osteosarcoma is the most common primary malignancy of the bone. Aminopeptidase N (APN/CD13), a Zn+2‑dependent ectopeptidase localized on the cell surface, is widely considered to influence the invasion mechanism. This study explores the potential involvement of APN in migration and invasion of human osteosarcoma cells in vitro using inhi­bitors and activators of APN. Cells treated with APN inhibitor bestatin displayed decreased migration and invasion in a Boyden chamber Transwell assay. Western blotting revealed reduced levels of mitogen‑activated protein kinase (MAPK) and phosphatidylinositol 3‑kinase (PI3K) pathway proteins, reduced phosphorylation of p38, ERK1/2 and JNK and decreased levels of NF‑κB. Bestatin treatment also lowered APN, matrix metalloproteinase (MMP)‑2 and ‑9 enzymatic activity and their mRNA expression. Reduced MMP‑2 and ‑9 protein levels were also observed. By comparison, cells treated with cytokine interleukin‑6 (IL‑6), a stimulator of APN, displayed increased migration and invasion. Western blotting revealed increased levels of MAPK and PI3K pathway proteins, phosphorylated p38, ERK1/2 and JNK, and NF‑κB. IL‑6 treatment also increased APN and MMP‑2 and ‑9 enzymatic activity. An increase of APN, MMP‑2 and ‑9 mRNA levels, and MMP‑2 and ‑9 protein levels was also observed. Together these experiments reveal potential enzymatic and signalling roles for APN in osteosarcoma and establish a starting point for an in‑depth analysis of the role of APN in regulating invasiveness. A deeper knowledge about the regulatory mechanisms of APN may contribute to the development of anti‑metastatic therapies.
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1 

Ottaviani G and Jaffe N: The epidemiology of osteosarcoma. Cancer Treat Res. 152:3–13. 2009. View Article : Google Scholar

2 

Sung L, Anderson JR, Donaldson SS, Spunt SL, Crist WM and Pappo AS; Soft Tissue Sarcoma Committee of the Children’s Oncology Group. Late events occurring five years or more after successful therapy for childhood rhabdomyosarcoma: a report from the Soft Tissue Sarcoma Committee of the Children’s Oncology Group. Eur J Cancer. 40:1878–1885. 2004.

3 

Cheng YY, Huang L, Lee KM, Li K and Kumta SM: Alendronate regulates cell invasion and MMP-2 secretion in human osteosarcoma cell lines. Pediatr Blood Cancer. 42:410–415. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Cho HJ, Lee TS, Park JB, et al: Disulfiram suppresses invasive ability of osteosarcoma cells via the inhibition of MMP-2 and MMP-9 expression. J Biochem Mol Biol. 40:1069–1076. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Xin ZF, Kim YK and Jung ST: Risedronate inhibits human osteosarcoma cell invasion. J Exp Clin Cancer Res. 28:1052009. View Article : Google Scholar : PubMed/NCBI

6 

Fan DG, Dai JY, Tang J, et al: Silencing of calpain expression reduces the metastatic potential of human osteosarcoma cells. Cell Biol Int. 33:1263–1267. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Dass CR, Nadesapillai AP, Robin D, et al: Downregulation of uPAR confirms link in growth and metastasis of osteosarcoma. Clin Exp Metastasis. 22:643–652. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Wan X, Kim SY, Guenther LM, et al: Beta4 integrin promotes osteosarcoma metastasis and interacts with ezrin. Oncogene. 28:3401–3411. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Haydon RC, Deyrup A, Ishikawa A, et al: Cytoplasmic and/or nuclear accumulation of the beta-catenin protein is a frequent event in human osteosarcoma. Int J Cancer. 102:338–342. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Iwaya K, Ogawa H, Kuroda M, Izumi M, Ishida T and Mukai K: Cytoplasmic and/or nuclear staining of beta-catenin is associated with lung metastasis. Clin Exp Metastasis. 20:525–529. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Engin F, Bertin T, Ma O, et al: Notch signaling contributes to the pathogenesis of human osteosarcomas. Hum Mol Genet. 18:1464–1470. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Hughes DP: How the NOTCH pathway contributes to the ability of osteosarcoma cells to metastasize. Cancer Treat Res. 152:479–496. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Leow PC, Tian Q, Ong ZY, Yang Z and Ee PL: Antitumor activity of natural compounds, curcumin and PKF118-310, as Wnt/β-catenin antagonists against human osteosarcoma cells. Invest New Drugs. 28:766–782. 2010.PubMed/NCBI

14 

Li Y, Zhang J, Ma D, et al: Curcumin inhibits proliferation and invasion of osteosarcoma cells through inactivation of Notch-1 signaling. FEBS J. 279:2247–2259. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Lin YM, Chang ZL, Liao YY, Chou MC and Tang CH: IL-6 promotes ICAM-1 expression and cell motility in human osteosarcoma. Cancer Lett. 328:135–143. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Berdiaki A, Datsis GA, Nikitovic D, et al: Parathyroid hormone (PTH) peptides through the regulation of hyaluronan metabolism affect osteosarcoma cell migration. IUBMB Life. 62:377–386. 2010.PubMed/NCBI

17 

Yang R, Hoang BH, Kubo T, et al: Over-expression of parathyroid hormone Type 1 receptor confers an aggressive phenotype in osteosarcoma. Int J Cancer. 121:943–954. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Dobashi Y, Watanabe H, Matsubara M, et al: Autocrine motility factor/glucose-6-phosphate isomerase is a possible predictor of metastasis in bone and soft tissue tumours. J Pathol. 208:44–53. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Niinaka Y, Harada K, Fujimuro M, et al: Silencing of autocrine motility factor induces mesenchymal-to-epithelial transition and suppression of osteosarcoma pulmonary metastasis. Cancer Res. 70:9483–9493. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Fromigue O, Hamidouche Z, Vaudin P, et al: CYR61 downregulation reduces osteosarcoma cell invasion, migration, and metastasis. J Bone Miner Res. 26:1533–1542. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Bauvois B: Transmembrane proteases in cell growth and invasion: new contributors to angiogenesis? Oncogene. 23:317–329. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Carl-McGrath S, Lendeckel U, Ebert M and Röcken C: Ectopeptidases in tumour biology: a review. Histol Histopathol. 21:1339–1353. 2006.

23 

Hitzerd SM, Verbrugge SE, Ossenkoppele G, Jansen G and Peters GJ: Positioning of aminopeptidase inhibitors in next generation cancer therapy. Amino Acids. 46:793–808. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Kido A, Krueger S, Haeckel C and Roessner A: Possible contribution of aminopeptidase N (APN/CD13) to invasive potential enhanced by interleukin-6 and soluble interleukin-6 receptor in human osteosarcoma cell lines. Clin Exp Metastasis. 17:857–863. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Fujii H, Nakajima M, Saiki I, Yoneda J, Azuma I and Tsuruo T: Human melanoma invasion and metastasis enhancement by high expression of aminopeptidase N/CD13. Clin Exp Metastasis. 13:337–344. 1995. View Article : Google Scholar : PubMed/NCBI

26 

Kehlen A, Lendeckel U, Dralle H, Langner J and Hoang-Vu C: Biological significance of aminopeptidase N/CD13 in thyroid carcinomas. Cancer Res. 63:8500–8506. 2003.PubMed/NCBI

27 

Petrovic N, Schacke W, Gahagan JR, et al: CD13/APN regulates endothelial invasion and filopodia formation. Blood. 110:142–150. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Hong DY, Lee BJ, Lee JC, Choi JS, Wang SG and Ro JH: Expression of VEGF, HGF, IL-6, IL-8, MMP-9, telomerase in peripheral blood of patients with head and neck squamous cell carcinoma. Clin Exp Otorhinolaryngol. 2:186–192. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Loo WT, Sasano H and Chow LW: Pro-inflammatory cytokine, matrix metalloproteinases and TIMP-1 are involved in wound healing after mastectomy in invasive breast cancer patients. Biomed Pharmacother. 61:548–552. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Güllü IH, Kurdoğlu M and Akalin I: The relation of gelatinase (MMP-2 and -9) expression with distant site metastasis and tumour aggressiveness in colorectal cancer. Br J Cancer. 82:2492000.PubMed/NCBI

31 

Mizutani K, Kofuji K and Shirouzu K: The significance of MMP-1 and MMP-2 in peritoneal disseminated metastasis of gastric cancer. Surg Today. 30:614–621. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Korpi JT, Hagström J, Lehtonen N, et al: Expression of matrix metalloproteinases-2, −8, −13, −26, and tissue inhibitors of metalloproteinase-1 in human osteosarcoma. Surg Oncol. 20:e18–e22. 2011.

33 

Ferrari C, Benassi S, Ponticelli F, et al: Role of MMP-9 and its tissue inhibitor TIMP-1 in human osteosarcoma: findings in 42 patients followed for 1–16 years. Acta Orthop Scand. 75:487–491. 2004.PubMed/NCBI

34 

Foukas AF, Deshmukh NS, Grimer RJ, Mangham DC, Mangos EG and Taylor S: Stage-IIB osteosarcomas around the knee. A study of MMP-9 in surviving tumour cells. J Bone Joint Surg Br. 84:706–711. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Himelstein BP, Asada N, Carlton MR and Collins MH: Matrix metalloproteinase-9 (MMP-9) expression in childhood osseous osteosarcoma. Med Pediatr Oncol. 31:471–474. 1998. View Article : Google Scholar : PubMed/NCBI

36 

Bellido T, O’Brien CA, Roberson PK and Manolagas SC: Transcriptional activation of the p21(WAF1,CIP1,SDI1) gene by interleukin-6 type cytokines. A prerequisite for their pro-differentiating and anti-apoptotic effects on human osteoblastic cells. J Biol Chem. 273:21137–21144. 1998. View Article : Google Scholar : PubMed/NCBI

37 

Franchimont N, Gangji V, Durant D and Canalis E: Interleukin-6 with its soluble receptor enhances the expression of insulin-like growth factor-I in osteoblasts. Endocrinology. 138:5248–5255. 1997.PubMed/NCBI

38 

Jilka RL, Weinstein RS, Bellido T, Parfitt AM and Manolagas SC: Osteoblast programmed cell death (apoptosis): modulation by growth factors and cytokines. J Bone Miner Res. 13:793–802. 1998. View Article : Google Scholar : PubMed/NCBI

39 

Nishimura R, Moriyama K, Yasukawa K, Mundy GR and Yoneda T: Combination of interleukin-6 and soluble interleukin-6 receptors induces differentiation and activation of JAK-STAT and MAP kinase pathways in MG-63 human osteoblastic cells. J Bone Miner Res. 13:777–785. 1998. View Article : Google Scholar : PubMed/NCBI

40 

Kehlen A, Göhring B, Langner J and Riemann D: Regulation of the expression of aminopeptidase A, aminopeptidase N/CD13 and dipeptidylpeptidase IV/CD26 in renal carcinoma cells and renal tubular epithelial cells by cytokines and cAMP-increasing mediators. Clin Exp Immunol. 111:435–441. 1998. View Article : Google Scholar

41 

Chen YY, Chiang SY, Lin JG, et al: Emodin, aloe-emodin and rhein inhibit migration and invasion in human tongue cancer SCC-4 cells through the inhibition of gene expression of matrix metalloproteinase-9. Int J Oncol. 36:1113–1120. 2010.PubMed/NCBI

42 

Lin CC, Chen JT, Yang JS, et al: Danthron inhibits the migration and invasion of human brain glioblastoma multiforme cells through the inhibition of mRNA expression of focal adhesion kinase, Rho kinases-1 and metalloproteinase-9. Oncol Rep. 22:1033–1037. 2009.

43 

Lai KC, Huang AC, Hsu SC, et al: Benzyl isothiocyanate (BITC) inhibits migration and invasion of human colon cancer HT29 cells by inhibiting matrix metalloproteinase-2/-9 and urokinase plasminogen (uPA) through PKC and MAPK signaling pathway. J Agric Food Chem. 58:2935–2942. 2010. View Article : Google Scholar

44 

Liu KC, Huang AC, Wu PP, et al: Gallic acid suppresses the migration and invasion of PC-3 human prostate cancer cells via inhibition of matrix metalloproteinase-2 and -9 signaling pathways. Oncol Rep. 26:177–184. 2011.PubMed/NCBI

45 

Chiang JH, Yang JS, Ma CY, et al: Danthron, an anthraquinone derivative, induces DNA damage and caspase cascades-mediated apoptosis in SNU-1 human gastric cancer cells through mitochondrial permeability transition pores and Bax-triggered pathways. Chem Res Toxicol. 24:20–29. 2011. View Article : Google Scholar

46 

Wen YF, Yang JS, Kuo SC, et al: Investigation of anti-leukemia molecular mechanism of ITR-284, a carboxamide analog, in leukemia cells and its effects in WEHI-3 leukemia mice. Biochem Pharmacol. 79:389–398. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Hennessy BT, Smith DL, Ram PT, Lu Y and Mills GB: Exploiting the PI3K/AKT pathway for cancer drug discovery. Nat Rev Drug Discov. 4:988–1004. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Qi M and Elion EA: MAP kinase pathways. J Cell Sci. 118:3569–3572. 2005. View Article : Google Scholar

49 

Bond M, Chase AJ, Baker AH and Newby AC: Inhibition of transcription factor NF-kappaB reduces matrix metalloproteinase-1, -3 and -9 production by vascular smooth muscle cells. Cardiovasc Res. 50:556–565. 2001. View Article : Google Scholar : PubMed/NCBI

50 

Bauvois B and Dauzonne D: Aminopeptidase-N/CD13 (EC 3.4.11.2) inhibitors: chemistry, biological evaluations, and therapeutic prospects. Med Res Rev. 26:88–130. 2006. View Article : Google Scholar : PubMed/NCBI

51 

Balkwill F, Charles KA and Mantovani A: Smoldering and polarized inflammation in the initiation and promotion of malignant disease. Cancer Cell. 7:211–217. 2005. View Article : Google Scholar : PubMed/NCBI

52 

Lo CW, Chen MW, Hsiao M, et al: IL-6 trans-signaling in formation and progression of malignant ascites in ovarian cancer. Cancer Res. 71:424–434. 2011. View Article : Google Scholar : PubMed/NCBI

53 

Tang CH, Chen CF, Chen WM and Fong YC: IL-6 increases MMP-13 expression and motility in human chondrosarcoma cells. J Biol Chem. 286:11056–11066. 2011. View Article : Google Scholar : PubMed/NCBI

54 

Tu B, Du L, Fan QM, Tang Z and Tang TT: STAT3 activation by IL-6 from mesenchymal stem cells promotes the proliferation and metastasis of osteosarcoma. Cancer Lett. 325:80–88. 2012. View Article : Google Scholar : PubMed/NCBI

55 

Mina-Osorio P: The moonlighting enzyme CD13: old and new functions to target. Trends Mol Med. 14:361–371. 2008. View Article : Google Scholar : PubMed/NCBI

56 

Liao CL, Lai KC, Huang AC, et al: Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways. Food Chem Toxicol. 50:1734–1740. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liang W, Gao B, Xu G, Weng D, Xie M and Qian Y: Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines. Int J Oncol 45: 2475-2485, 2014.
APA
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., & Qian, Y. (2014). Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines. International Journal of Oncology, 45, 2475-2485. https://doi.org/10.3892/ijo.2014.2664
MLA
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., Qian, Y."Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines". International Journal of Oncology 45.6 (2014): 2475-2485.
Chicago
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., Qian, Y."Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines". International Journal of Oncology 45, no. 6 (2014): 2475-2485. https://doi.org/10.3892/ijo.2014.2664
Copy and paste a formatted citation
x
Spandidos Publications style
Liang W, Gao B, Xu G, Weng D, Xie M and Qian Y: Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines. Int J Oncol 45: 2475-2485, 2014.
APA
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., & Qian, Y. (2014). Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines. International Journal of Oncology, 45, 2475-2485. https://doi.org/10.3892/ijo.2014.2664
MLA
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., Qian, Y."Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines". International Journal of Oncology 45.6 (2014): 2475-2485.
Chicago
Liang, W., Gao, B., Xu, G., Weng, D., Xie, M., Qian, Y."Possible contribution of aminopeptidase N (APN/CD13) to migration and invasion of human osteosarcoma cell lines". International Journal of Oncology 45, no. 6 (2014): 2475-2485. https://doi.org/10.3892/ijo.2014.2664
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