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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma

  • Authors:
    • Yang-Ki Minn
    • Da Hye Lee
    • Woo Jin Hyung
    • Ji Eun Kim
    • Junjeong Choi
    • Seung-Ho Yang
    • Hotaek Song
    • Beom Jin Lim
    • Se Hoon Kim
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Kangnam Sacred Heart Hospital, Hallym University, Seoul, Republic of Korea, Department of Pathology, Yonsei University, College of Medicine, Seoul, Republic of Korea, Department of Surgery, Yonsei University, College of Medicine, Seoul, Republic of Korea, Department of Pathology, Seoul National University, Boramae Hospital, Seoul, Republic of Korea, Department of Pathology, Yonsei University, Wonju College of Medicine, Wonju, Republic of Korea, Department of Neurosurgery, St. Vincent Hospital, The Catholic University of Korea, Suwon, Republic of Korea, Department of Radiology and Research Institute of Radiologic Science, Yonsei University, College of Medicine, Seoul, Republic of Korea
  • Pages: 2403-2410
    |
    Published online on: September 26, 2014
       https://doi.org/10.3892/ijo.2014.2680
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Abstract

Although the incidence of brain metastasis in gastric cancer is relatively low, its prevalence may increase with improved therapy and longer survival tumors. The molecular mechanisms underlying brain metastases are not well understood. To gain insight into the mechanism of brain metastasis, we studied differences in microRNA (miRNA) expression levels in 8 cases of matched primary gastric adenocarcinoma and brain metastatic adenocarcinoma using the Illumina microRNA microarray chip. We identified 6 upregulated and 2 downregulated miRNAs in all 8 cases simultaneously. Interestingly, 2 out of 8 miRNAs (hsa-miR‑141-3p and hsa-miR-200b-3p) belonged to the miR-200 family. Online microRNA database searching revealed that ZEB2 is the top-ranked target gene for hsa-miR141-3p and hsa-miR-200b-3p, prompting us to focus ZEB2 expression in brain metastatic adenocarcinoma. We confirmed that ZEB2 expression was markedly downregulated in some brain metastatic samples. In addition, decreased ZEB2 expression was noted by western blot analysis of 2 metastatic gastric adenocarcinoma cell types that were derived by in vivo selection following intracardiac injection of gastric cancer cell lines. In conclusion, we demonstrate that expression of miRNA-200 family members and ZEB2 are associated with brain metastases of gastric adenocarcinoma, not only in matched patient samples, but also in metastatic cell lines that were derived by in vivo selection.
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Copy and paste a formatted citation
Spandidos Publications style
Minn Y, Lee DH, Hyung WJ, Kim JE, Choi J, Yang S, Song H, Lim BJ and Kim SH: MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma. Int J Oncol 45: 2403-2410, 2014.
APA
Minn, Y., Lee, D.H., Hyung, W.J., Kim, J.E., Choi, J., Yang, S. ... Kim, S.H. (2014). MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma. International Journal of Oncology, 45, 2403-2410. https://doi.org/10.3892/ijo.2014.2680
MLA
Minn, Y., Lee, D. H., Hyung, W. J., Kim, J. E., Choi, J., Yang, S., Song, H., Lim, B. J., Kim, S. H."MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma". International Journal of Oncology 45.6 (2014): 2403-2410.
Chicago
Minn, Y., Lee, D. H., Hyung, W. J., Kim, J. E., Choi, J., Yang, S., Song, H., Lim, B. J., Kim, S. H."MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma". International Journal of Oncology 45, no. 6 (2014): 2403-2410. https://doi.org/10.3892/ijo.2014.2680
Copy and paste a formatted citation
x
Spandidos Publications style
Minn Y, Lee DH, Hyung WJ, Kim JE, Choi J, Yang S, Song H, Lim BJ and Kim SH: MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma. Int J Oncol 45: 2403-2410, 2014.
APA
Minn, Y., Lee, D.H., Hyung, W.J., Kim, J.E., Choi, J., Yang, S. ... Kim, S.H. (2014). MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma. International Journal of Oncology, 45, 2403-2410. https://doi.org/10.3892/ijo.2014.2680
MLA
Minn, Y., Lee, D. H., Hyung, W. J., Kim, J. E., Choi, J., Yang, S., Song, H., Lim, B. J., Kim, S. H."MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma". International Journal of Oncology 45.6 (2014): 2403-2410.
Chicago
Minn, Y., Lee, D. H., Hyung, W. J., Kim, J. E., Choi, J., Yang, S., Song, H., Lim, B. J., Kim, S. H."MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma". International Journal of Oncology 45, no. 6 (2014): 2403-2410. https://doi.org/10.3892/ijo.2014.2680
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