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International Journal of Oncology
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Article

Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues

  • Authors:
    • Andrej Nikoshkov
    • Kristina Broliden
    • Sanaz Attarha
    • Vitali Sviatoha
    • Ann‑Cathrin Hellström
    • Miriam Mints
    • Sonia Andersson
  • View Affiliations / Copyright

    Affiliations: Department of Women's and Children's Health, Division of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden, Department of Medicine Solna, Unit of Infectious Diseases, Center for Molecular Medicine, Karolinska Institute, Karolinska University Hospital, 171 76 Stockholm, Sweden, Department of Oncology‑Pathology, Karolinska Institute, 171 76 Stockholm, Sweden, Department of Gynecological Oncology, Radiumhemmet, Karolinska University Hospital, 171 76 Stockholm, Sweden
  • Pages: 107-112
    |
    Published online on: October 23, 2014
       https://doi.org/10.3892/ijo.2014.2724
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Abstract

Cervical cancer is the second most prevalent malignancy among women worldwide, and additional objective diagnostic markers for this disease are needed. Given the link between cancer development and alternative splicing, we aimed to analyze the splicing patterns of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes, which are associated with different cancers, in normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors, to identify new objective diagnostic markers. Biopsies of normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors, were subjected to rapid amplification of cDNA 3' ends (3' RACE) RT‑PCR. Resulting PCR products were analyzed on agarose gels, gel‑purified and sequenced. Normal cervical tissue, preinvasive cervical lesions and invasive cervical tumors contained one PCR product corresponding to full‑length PRDX2, RAB5A and RAB25 transcripts. All tissues contained two RAB1A‑specific PCR products corresponding to the full‑length transcript and one new alternatively spliced RAB1A transcript. Invasive cervical tumors contained one PCR product corresponding to the full‑length RAB1B transcript, while all normal cervical tissue and preinvasive cervical lesions contained both the full‑length RAB1B transcript and three new alternatively spliced RAB1B transcripts. Alternative splicing of the RAB1A transcript occurs in all cervical tissues, while alternative splicing of the RAB1B transcript occurs in normal cervical tissue and in preinvasive cervical lesions; not in invasive cervical tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Nikoshkov A, Broliden K, Attarha S, Sviatoha V, Hellström AC, Mints M and Andersson S: Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues. Int J Oncol 46: 107-112, 2015.
APA
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., & Andersson, S. (2015). Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues. International Journal of Oncology, 46, 107-112. https://doi.org/10.3892/ijo.2014.2724
MLA
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., Andersson, S."Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues". International Journal of Oncology 46.1 (2015): 107-112.
Chicago
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., Andersson, S."Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues". International Journal of Oncology 46, no. 1 (2015): 107-112. https://doi.org/10.3892/ijo.2014.2724
Copy and paste a formatted citation
x
Spandidos Publications style
Nikoshkov A, Broliden K, Attarha S, Sviatoha V, Hellström AC, Mints M and Andersson S: Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues. Int J Oncol 46: 107-112, 2015.
APA
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., & Andersson, S. (2015). Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues. International Journal of Oncology, 46, 107-112. https://doi.org/10.3892/ijo.2014.2724
MLA
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., Andersson, S."Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues". International Journal of Oncology 46.1 (2015): 107-112.
Chicago
Nikoshkov, A., Broliden, K., Attarha, S., Sviatoha, V., Hellström, A., Mints, M., Andersson, S."Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5A and RAB25 genes in normal and cancer cervical tissues". International Journal of Oncology 46, no. 1 (2015): 107-112. https://doi.org/10.3892/ijo.2014.2724
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