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Article

Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells

  • Authors:
    • Qin Ru
    • Xiang Tian
    • Ming‑Shan Pi
    • Lin Chen
    • Kai Yue
    • Qi Xiong
    • Bao‑Miao Ma
    • Chao‑Ying Li
  • View Affiliations / Copyright

    Affiliations: Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, Hubei 430056, P.R. China
  • Pages: 833-840
    |
    Published online on: November 25, 2014
       https://doi.org/10.3892/ijo.2014.2777
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Abstract

Accumulating evidence has proved that potassium channels (K+ channels) are involved in regulating cell proliferation, cell cycle progression and apoptosis of tumor cells. However, the precise cellular mechanisms are still unknown. In the present study, we investigated the effect and mechanisms of quinidine, a commonly used voltage‑gated K+ channel blocker, on cell proliferation and apoptosis of human glioma U87‑MG cells. We found that quinidine significantly inhibited the proliferation of U87‑MG cells and induced apoptosis in a dose‑dependent manner. The results of caspase colorimetric assay showed that the mitochondrial pathway was the main mode involved in the quinidine‑induced apoptotic process. Furthermore, the concentration range of quinidine, which inhibited voltage‑gated K+ channel currents in electrophysiological assay, was consistent with that of quinidine inhibiting cell proliferation and inducing cell apoptosis. In U87‑MG cells treated with quinidine (100 µmol/l), 11 of 2,042 human microRNAs (miRNAs) were upregulated and 16 were downregulated as detected with the miRNA array analysis. The upregulation of miR‑149‑3p and downregulation of miR‑424‑5p by quinidine treatment were further verified by using quantitative real‑time PCR. In addition, using miRNA target prediction program, putative target genes related to cell prolif­eration and apoptosis for two differentially expressed miRNAs were predicted. Taken together, these data suggested that the anti‑proliferative and pro‑apoptosis effect of voltage‑gated K+ channel blocker quinidine in human glioma cells was mediated at least partly through regulating expression of miRNAs, and provided further support for the mechanisms of voltage‑gated K+ channels in mediating cell proliferation and apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Ru Q, Tian X, Pi MS, Chen L, Yue K, Xiong Q, Ma BM and Li CY: Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells. Int J Oncol 46: 833-840, 2015.
APA
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q. ... Li, C. (2015). Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells. International Journal of Oncology, 46, 833-840. https://doi.org/10.3892/ijo.2014.2777
MLA
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q., Ma, B., Li, C."Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells". International Journal of Oncology 46.2 (2015): 833-840.
Chicago
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q., Ma, B., Li, C."Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells". International Journal of Oncology 46, no. 2 (2015): 833-840. https://doi.org/10.3892/ijo.2014.2777
Copy and paste a formatted citation
x
Spandidos Publications style
Ru Q, Tian X, Pi MS, Chen L, Yue K, Xiong Q, Ma BM and Li CY: Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells. Int J Oncol 46: 833-840, 2015.
APA
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q. ... Li, C. (2015). Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells. International Journal of Oncology, 46, 833-840. https://doi.org/10.3892/ijo.2014.2777
MLA
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q., Ma, B., Li, C."Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells". International Journal of Oncology 46.2 (2015): 833-840.
Chicago
Ru, Q., Tian, X., Pi, M., Chen, L., Yue, K., Xiong, Q., Ma, B., Li, C."Voltage‑gated K+ channel blocker quinidine inhibits proliferation and induces apoptosis by regulating expression of microRNAs in human glioma U87‑MG cells". International Journal of Oncology 46, no. 2 (2015): 833-840. https://doi.org/10.3892/ijo.2014.2777
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