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Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas

  • Authors:
    • Ira Kokovic
    • Barbara Jezersek Novakovic
    • Srdjan Novakovic
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Diagnostics, Institute of Oncology Ljubljana, Ljubljana, Slovenia, Division of Medical Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia
    Copyright: © Kokovic et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 953-962
    |
    Published online on: December 9, 2014
       https://doi.org/10.3892/ijo.2014.2790
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Abstract

Analysis of the immunoglobulin κ light chain (IGK) gene is an alternative method for B-cell clonality assessment in the diagnosis of mature B-cell proliferations in which the detection of clonal immunoglobulin heavy chain (IGH) gene rearrangements fails. The aim of the present study was to evaluate the added value of standardized BIOMED-2 assay for the detection of clonal IGK gene rearrangements in the diagnostic setting of suspected B-cell lymphomas. With this purpose, 92 specimens from 80 patients with the final diagnosis of mature B-cell lymphoma (37 specimens), mature T-cell lymphoma (26 specimens) and reactive lymphoid proliferation (29 specimens) were analyzed for B-cell clonality. B-cell clonality analysis was performed using the BIOMED-2 IGH and IGK gene clonality assays. The determined sensitivity of the IGK assay was 67.6%, while the determined sensitivity of the IGH assay was 75.7%. The sensitivity of combined IGH+IGK assay was 81.1%. The determined specificity of the IGK assay was 96.2% in the group of T-cell lymphomas and 96.6% in the group of reactive lesions. The determined specificity of the IGH assay was 84.6% in the group of lymphomas and 86.2% in the group of reactive lesions. The comparison of GeneScan (GS) and heteroduplex pretreatment-polyacrylamide gel electrophoresis (HD-PAGE) methods for the analysis of IGK gene rearrangements showed a higher efficacy of GS analysis in a series of 27 B-cell lymphomas analyzed by both methods. In the present study, we demonstrated that by applying the combined IGH+IGK clonality assay the overall detection rate of B-cell clonality was increased by 5.4%. Thus, we confirmed the added value of the standardized BIOMED-2 IGK assay for assessment of B-cell clonality in suspected B-cell lymphomas with inconclusive clinical and cyto/histological diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Kokovic I, Jezersek Novakovic B and Novakovic S: Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas. Int J Oncol 46: 953-962, 2015.
APA
Kokovic, I., Jezersek Novakovic, B., & Novakovic, S. (2015). Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas. International Journal of Oncology, 46, 953-962. https://doi.org/10.3892/ijo.2014.2790
MLA
Kokovic, I., Jezersek Novakovic, B., Novakovic, S."Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas". International Journal of Oncology 46.3 (2015): 953-962.
Chicago
Kokovic, I., Jezersek Novakovic, B., Novakovic, S."Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas". International Journal of Oncology 46, no. 3 (2015): 953-962. https://doi.org/10.3892/ijo.2014.2790
Copy and paste a formatted citation
x
Spandidos Publications style
Kokovic I, Jezersek Novakovic B and Novakovic S: Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas. Int J Oncol 46: 953-962, 2015.
APA
Kokovic, I., Jezersek Novakovic, B., & Novakovic, S. (2015). Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas. International Journal of Oncology, 46, 953-962. https://doi.org/10.3892/ijo.2014.2790
MLA
Kokovic, I., Jezersek Novakovic, B., Novakovic, S."Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas". International Journal of Oncology 46.3 (2015): 953-962.
Chicago
Kokovic, I., Jezersek Novakovic, B., Novakovic, S."Diagnostic value of immunoglobulin κ light chain gene rearrangement analysis in B-cell lymphomas". International Journal of Oncology 46, no. 3 (2015): 953-962. https://doi.org/10.3892/ijo.2014.2790
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