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Article

Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells

  • Authors:
    • Min Young Kim
    • Seong-Joon Park
    • Jae Woong Shim
    • Kwangmo Yang
    • Ho Sung Kang
    • Kyu Heo
  • View Affiliations / Copyright

    Affiliations: Research Center, Dongnam Institute of Radiological and Medical Science (DIRAMS), Busan 619-953, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea
  • Pages: 1659-1666
    |
    Published online on: January 28, 2015
       https://doi.org/10.3892/ijo.2015.2857
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Abstract

Naphthazarin (Naph, DHNQ, 5,8-dihydroxy-l,4-naphthoquinone) is one of the naturally available 1,4-naphthoquinone derivatives that are well-known for their anti-inflammatory, antioxidant, antibacterial and antitumor cytotoxic effects in cancer cells. Herein, we investigated whether Naph has effects on cell cycle arrest and apoptosis in MCF-7 human breast cancer cells exposed to ionizing radiation (IR). Naph reduced the MCF-7 cell viability in a dose-dependent manner. We also found that Naph and/or IR increased the p53-dependent p21 (CIP/WAF1) promoter activity. Noteworthy, our ChIP assay results showed that Naph and IR combined treatment activated the p21 promoter via inhibition of binding of multi-domain proteins, DNMT1, UHRF1 and HDAC1. Apoptosis and cell cycle analyses demonstrated that Naph and IR combined treatment induced cell cycle arrest and apoptosis in MCF-7 cells. Herein, we showed that Naph treatment enhances IR-induced cell cycle arrest and death in MCF-7 human breast cancer cells through the p53-dependent p21 activation mechanism. These results suggest that Naph might sensitize breast cancer cells to radiotherapy by enhancing the p53-p21 mechanism activity.
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Copy and paste a formatted citation
Spandidos Publications style
Kim MY, Park S, Shim JW, Yang K, Kang HS and Heo K: Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells. Int J Oncol 46: 1659-1666, 2015.
APA
Kim, M.Y., Park, S., Shim, J.W., Yang, K., Kang, H.S., & Heo, K. (2015). Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells. International Journal of Oncology, 46, 1659-1666. https://doi.org/10.3892/ijo.2015.2857
MLA
Kim, M. Y., Park, S., Shim, J. W., Yang, K., Kang, H. S., Heo, K."Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells". International Journal of Oncology 46.4 (2015): 1659-1666.
Chicago
Kim, M. Y., Park, S., Shim, J. W., Yang, K., Kang, H. S., Heo, K."Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells". International Journal of Oncology 46, no. 4 (2015): 1659-1666. https://doi.org/10.3892/ijo.2015.2857
Copy and paste a formatted citation
x
Spandidos Publications style
Kim MY, Park S, Shim JW, Yang K, Kang HS and Heo K: Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells. Int J Oncol 46: 1659-1666, 2015.
APA
Kim, M.Y., Park, S., Shim, J.W., Yang, K., Kang, H.S., & Heo, K. (2015). Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells. International Journal of Oncology, 46, 1659-1666. https://doi.org/10.3892/ijo.2015.2857
MLA
Kim, M. Y., Park, S., Shim, J. W., Yang, K., Kang, H. S., Heo, K."Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells". International Journal of Oncology 46.4 (2015): 1659-1666.
Chicago
Kim, M. Y., Park, S., Shim, J. W., Yang, K., Kang, H. S., Heo, K."Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells". International Journal of Oncology 46, no. 4 (2015): 1659-1666. https://doi.org/10.3892/ijo.2015.2857
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