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Article

β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression

  • Authors:
    • Chengcheng Zhao
    • Meng Zhang
    • Wenlan Liu
    • Chuanfang Wang
    • Qiusheng Zhang
    • Weiping Li
  • View Affiliations / Copyright

    Affiliations: Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China, Shenzhen Key Laboratory of Neurosurgery, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China
  • Pages: 1643-1650
    |
    Published online on: February 2, 2015
       https://doi.org/10.3892/ijo.2015.2862
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Abstract

Pituitary adenomas are among the most prevalent forms of intrinsic brain tumors. Although most pituitary adenomas are benign, some of them may become invasive and cause significant mass effect and hormonal dysfunction. We have previously shown that β-catenin is overexpressed in human pituitary adenomas and its level correlates to tumor grades. In the present study, we further investigated the role of β-catenin in pituitary adenoma cell proliferation and invasion in vitro. Stable β-catenin knockdown pituitary adenoma cell line was created by transfecting mouse growth hormone pituitary adenoma GT1.1 cells with β-catenin shRNA plasmid. Cell proliferation and invasion were assessed using CCK-8 kit and transwell assay, respectively. Our data demonstrated that knockdown of β-catenin with shRNA significantly inhibited the proliferation and invasion of GT1.1 cells. In β-catenin shRNA transfected cells, the expression of AKT, STAT3, cyclin D1 and CDK4 were significantly suppressed, which accounted for the observed growth retardation following β-catenin shRNA transfection. Moreover, β-catenin shRNA transfection led to a drastic reduction in MMP-2/9 secretion into the conditioned media, which might be responsible for the reduced invasiveness of β-catenin shRNA-transfected pituitary adenoma cells. These results indicate that β-catenin may regulate the expression of AKT, STAT3, cyclin D1, CDK4 and MMP-2/9 to promote pituitary adenoma cell proliferation and invasion.
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1 

Vandeva S, Jaffrain-Rea ML, Daly AF, Tichomirowa M, Zacharieva S and Beckers A: The genetics of pituitary adenomas. Best Pract Res Clin Endocrinol Metab. 24:461–476. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Melmed S: Pathogenesis of pituitary tumors. Nat Rev Endocrinol. 7:257–266. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Asa SL and Ezzat S: The pathogenesis of pituitary tumors. Annu Rev Pathol. 4:97–126. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Douglas KR, Brinkmeier ML, Kennell JA, et al: Identification of members of the Wnt signaling pathway in the embryonic pituitary gland. Mamm Genome. 12:843–851. 2001. View Article : Google Scholar

5 

Brinkmeier ML, Potok MA, Cha KB, et al: TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17:2152–2161. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Kioussi C, Briata P, Baek SH, et al: Identification of a Wnt/Dvl/beta-Catenin → Pitx2 pathway mediating cell-type-specific proliferation during development. Cell. 111:673–685. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Brinkmeier ML, Potok MA, Davis SW and Camper SA: TCF4 deficiency expands ventral diencephalon signaling and increases induction of pituitary progenitors. Dev Biol. 311:396–407. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Zeng YA and Nusse R: Wnt proteins are self-renewal factors for mammary stem cells and promote their long-term expansion in culture. Cell Stem Cell. 6:568–577. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Polakis P: Wnt signaling and cancer. Genes Dev. 14:1837–1851. 2000.PubMed/NCBI

10 

Reed KR, Athineos D, Meniel VS, et al: B-catenin deficiency, but not Myc deletion, suppresses the immediate phenotypes of APC loss in the liver. Proc Natl Acad Sci USA. 105:18919–18923. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Nelson WJ and Nusse R: Convergence of Wnt, beta-catenin, and cadherin pathways. Science. 303:1483–1487. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Willert K and Jones KA: Wnt signaling: is the party in the nucleus? Genes Dev. 20:1394–1404. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Gaston-Massuet C, Andoniadou CL, Signore M, et al: Increased Wingless (Wnt) signaling in pituitary progenitor/stem cells gives rise to pituitary tumors in mice and humans. Proc Natl Acad Sci USA. 108:11482–11487. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Li W, Zhang Y, Zhang M, Huang G and Zhang Q: Wnt4 is over-expressed in human pituitary adenomas and is associated with tumor invasion. J Clin Neurosci. 21:137–141. 2014. View Article : Google Scholar

15 

Benjamin JM and Nelson WJ: Bench to bedside and back again: molecular mechanisms of alpha-catenin function and roles in tumorigenesis. Semin Cancer Biol. 18:53–64. 2008. View Article : Google Scholar

16 

Kalani MY, Cheshier SH, Cord BJ, et al: Wnt-mediated self-renewal of neural stem/progenitor cells. Proc Natl Acad Sci USA. 105:16970–16975. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Nusse R: Wnt signaling and stem cell control. Cell Res. 18:523–527. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Logan CY and Nusse R: The Wnt signaling pathway in development and disease. Annu RevCell Dev Biol. 20:781–810. 2004. View Article : Google Scholar

19 

Cadigan KM and Peifer M: Wnt signaling from development to disease: insights from model systems. Cold Spring Harb Perspect Biol. 1:a0028812009. View Article : Google Scholar :

20 

Park GB, Kim DJ, Kim YS, Lee HK, Kim CW and Hur DY: Silencing of galectin-3 represses osteosarcoma cell migration and invasion through inhibition of FAK/Src/Lyn activation and beta-catenin expression and increases susceptibility to chemotherapeutic agents. Int J Oncol. 46:185–194. 2015.

21 

Bai XL, Zhang Q, Ye LY, et al: Myocyte enhancer factor 2C regulation of hepatocellular carcinoma via vascular endothelial growth factor and Wnt/beta-catenin signaling. Oncogene. Oct 20–2014.(Epub ahead of print). View Article : Google Scholar

22 

Salaroli R, Ronchi A, Buttarelli FR, et al: Wnt activation affects proliferation, invasiveness and radiosensitivity in medulloblastoma. J Neurooncol. Sep 28–2014.(Epub ahead of print). PubMed/NCBI

23 

Gao ZH, Lu C, Wang MX, Han Y and Guo LJ: Differential beta-catenin expression levels are associated with morphological features and prognosis of colorectal cancer. Oncol Lett. 8:2069–2076. 2014.PubMed/NCBI

24 

Sareddy GR, Panigrahi M, Challa S, Mahadevan A and Babu PP: Activation of Wnt/beta-catenin/Tcf signaling pathway in human astrocytomas. Neurochem Int. 55:307–317. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Trovato M, Torre ML, Ragonese M, et al: HGF/c-met system targeting PI3K/AKT and STAT3/phosphorylated-STAT3 pathways in pituitary adenomas: an immunohistochemical characterization in view of targeted therapies. Endocrine. 44:735–743. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Ma PC, Tretiakova MS, Nallasura V, Jagadeeswaran R, Husain AN and Salgia R: Downstream signalling and specific inhibition of c-MET/HGF pathway in small cell lung cancer: implications for tumour invasion. Br J Cancer. 97:368–377. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Liotta LA and Kohn EC: The microenvironment of the tumour-host interface. Nature. 411:375–379. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Coussens LM and Werb Z: Inflammation and cancer. Nature. 420:860–867. 2002. View Article : Google Scholar : PubMed/NCBI

29 

de Aguiar PH, Aires R, Laws ER, et al: Labeling index in pituitary adenomas evaluated by means of MIB-1: is there a prognostic role? A critical review. Neurol Res. 32:1060–1071. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Cully M, You H, Levine AJ and Mak TW: Beyond PTEN mutations: the PI3K pathway as an integrator of multiple inputs during tumorigenesis. Nat Rev Cancer. 6:184–192. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Fu Y, Zhang Q, Kang C, et al: Inhibitory effects of adenovirus mediated COX-2, Akt1 and PIK3R1 shRNA on the growth of malignant tumor cells in vitro and in vivo. Int J Oncol. 35:583–591. 2009.PubMed/NCBI

32 

Yue X, Lan F, Yang W, et al: Interruption of beta-catenin suppresses the EGFR pathway by blocking multiple oncogenic targets in human glioma cells. Brain Res. 1366:27–37. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Dechow TN, Pedranzini L, Leitch A, et al: Requirement of matrix metalloproteinase-9 for the transformation of human mammary epithelial cells by Stat3-C. Proc Natl Acad Sci USA. 101:10602–10607. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Silver DL, Naora H, Liu J, Cheng W and Montell DJ: Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility. Cancer Res. 64:3550–3558. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Senft C, Priester M, Polacin M, et al: Inhibition of the JAK-2/STAT3 signaling pathway impedes the migratory and invasive potential of human glioblastoma cells. J Neurooncol. 101:393–403. 2011. View Article : Google Scholar

36 

Wang J, Li X, Lu X and Pi L: The regulation of stat3 signal transduction pathway to G1 to S phase of laryngocarcinoma cell. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 22:699–703. 2008.(In Chinese). PubMed/NCBI

37 

Liu W, Kunishio K, Matsumoto Y, Okada M and Nagao S: Matrix metalloproteinase-2 expression correlates with cavernous sinus invasion in pituitary adenomas. J Clin Neurosci. 12:791–794. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Gong J, Zhao Y, Abdel-Fattah R, et al: Matrix metallopro-teinase-9, a potential biological marker in invasive pituitary adenomas. Pituitary. 11:37–48. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhao C, Zhang M, Liu W, Wang C, Zhang Q and Li W: β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression. Int J Oncol 46: 1643-1650, 2015.
APA
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., & Li, W. (2015). β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression. International Journal of Oncology, 46, 1643-1650. https://doi.org/10.3892/ijo.2015.2862
MLA
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., Li, W."β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression". International Journal of Oncology 46.4 (2015): 1643-1650.
Chicago
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., Li, W."β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression". International Journal of Oncology 46, no. 4 (2015): 1643-1650. https://doi.org/10.3892/ijo.2015.2862
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao C, Zhang M, Liu W, Wang C, Zhang Q and Li W: β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression. Int J Oncol 46: 1643-1650, 2015.
APA
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., & Li, W. (2015). β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression. International Journal of Oncology, 46, 1643-1650. https://doi.org/10.3892/ijo.2015.2862
MLA
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., Li, W."β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression". International Journal of Oncology 46.4 (2015): 1643-1650.
Chicago
Zhao, C., Zhang, M., Liu, W., Wang, C., Zhang, Q., Li, W."β-catenin knockdown inhibits pituitary adenoma cell proliferation and invasion via interfering with AKT and gelatinases expression". International Journal of Oncology 46, no. 4 (2015): 1643-1650. https://doi.org/10.3892/ijo.2015.2862
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