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Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile

  • Authors:
    • Kengo Kuroda
    • Tomokazu Fukuda
    • Hiroshi Isogai
    • Kazuhiko Okumura
    • Marija Krstic-Demonacos
    • Emiko Isogai
  • View Affiliations / Copyright

    Affiliations: Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan, Animal Research Center, Sapporo Medical University, Sapporo 060-8556, Japan, Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan, School of Environment and Life Sciences, College of Science and Technology, Cockcroft Building, University of Salford, Salford M5 4WT, UK
    Copyright: © Kuroda et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1516-1526
    |
    Published online on: February 10, 2015
       https://doi.org/10.3892/ijo.2015.2887
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Abstract

Metabolic reprogramming is one of the hallmarks of cancer and can be targeted by therapeutic agents. We previously reported that cathelicidin-related or modified antimicrobial peptides, such as FF/CAP18, have antiproliferative effects on the squamous cell carcinoma cell line SAS-H1, and the colon carcinoma cell line HCT116. Although antimicrobial peptides have potential use in the development of new therapeutic strategies, their effects on the metabolism of cancer cells are poorly understood. Here, we investigated changes in the levels of metabolites in HCT116 cells caused by FF/CAP18, via capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Analysis of the 177 intracellular metabolites and 113 metabolites in conditioned medium that were detected by CE-TOFMS, revealed dramatic changes in the metabolic profile of HCT116 cells after treatment with FF/CAP18. The metabolic profile showed that the levels of most metabolites in the major metabolic pathways supported the rapid proliferation of cancer cells. Purine metabolism, glycolysis, and the TCA cycle, were altered in FF/CAP18-treated cells in a dose-dependent manner. Our present study provides mechanistic insights into the anticancer effects of antimicrobial peptides that show great potential as new therapies for colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kuroda K, Fukuda T, Isogai H, Okumura K, Krstic-Demonacos M and Isogai E: Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile. Int J Oncol 46: 1516-1526, 2015.
APA
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., & Isogai, E. (2015). Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile. International Journal of Oncology, 46, 1516-1526. https://doi.org/10.3892/ijo.2015.2887
MLA
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., Isogai, E."Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile". International Journal of Oncology 46.4 (2015): 1516-1526.
Chicago
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., Isogai, E."Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile". International Journal of Oncology 46, no. 4 (2015): 1516-1526. https://doi.org/10.3892/ijo.2015.2887
Copy and paste a formatted citation
x
Spandidos Publications style
Kuroda K, Fukuda T, Isogai H, Okumura K, Krstic-Demonacos M and Isogai E: Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile. Int J Oncol 46: 1516-1526, 2015.
APA
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., & Isogai, E. (2015). Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile. International Journal of Oncology, 46, 1516-1526. https://doi.org/10.3892/ijo.2015.2887
MLA
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., Isogai, E."Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile". International Journal of Oncology 46.4 (2015): 1516-1526.
Chicago
Kuroda, K., Fukuda, T., Isogai, H., Okumura, K., Krstic-Demonacos, M., Isogai, E."Antimicrobial peptide FF/CAP18 induces apoptotic cell death in HCT116 colon cancer cells via changes in the metabolic profile". International Journal of Oncology 46, no. 4 (2015): 1516-1526. https://doi.org/10.3892/ijo.2015.2887
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