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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Modulation of the cancer cell transcriptome by culture media formulations and cell density

  • Authors:
    • Seung Wook Kim
    • Sun‑Jin Kim
    • Robert R. Langley
    • Isaiah J. Fidler
  • View Affiliations / Copyright

    Affiliations: Department of Cancer Biology, Metastasis Research Laboratory, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
  • Pages: 2067-2075
    |
    Published online on: March 17, 2015
       https://doi.org/10.3892/ijo.2015.2930
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Abstract

We investigated how varying the composition of cell culture formulations and growing cancer cells at different densities might affect tumor cell genotype. Specifically, we compared gene expression profiles generated by human MDA‑MB‑231 breast cancer cells cultured in different media [minimum essential medium (MEM), Dulbecco's modified Eagle's medium (DMEM), or Roswell Park Memorial Institute (RPMI)‑1640 medium] containing different concentrations of fetal bovine serum (FBS) or different sera (equine or bovine) that were grown at different cell densities. More than 2,000 genes were differentially modulated by at least a 2‑fold difference when MDA‑MB‑231 cancer cells were 90% confluent and compared with cultures that were 50% confluent. Altering the concentration of serum produced an even more pronounced effect on MDA‑MB‑231 cancer cell gene expression in that 2,981 genes were differentially expressed in a comparison between cells cultured in 0.1% FBS and same cell density cultures that were maintained in 10% FBS. A comparison between MDA‑MB‑231 cancer cells that were 90% confluent in MEM, DMEM, or RPMI‑1640 media, all containing 10% FBS, resulted in 8,925 differentially expressed genes. Moreover, one‑quarter (25.6%) of genes from our genome‑wide expression analysis were expressed at significantly different levels by cells grown in MEM, DMEM, or RPMI‑1640 media. Genes associated with epithelial‑mes­enchymal transition (EMT) were among the genes that were differentially modulated by cells grown in different cell culture formulations and these genes were verified at the protein level. Collectively, these results underscore the importance of accurate reporting and maintenance of uniform culture conditions to ensure reproducible results.
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Copy and paste a formatted citation
Spandidos Publications style
Kim SW, Kim SJ, Langley RR and Fidler IJ: Modulation of the cancer cell transcriptome by culture media formulations and cell density. Int J Oncol 46: 2067-2075, 2015.
APA
Kim, S.W., Kim, S., Langley, R.R., & Fidler, I.J. (2015). Modulation of the cancer cell transcriptome by culture media formulations and cell density. International Journal of Oncology, 46, 2067-2075. https://doi.org/10.3892/ijo.2015.2930
MLA
Kim, S. W., Kim, S., Langley, R. R., Fidler, I. J."Modulation of the cancer cell transcriptome by culture media formulations and cell density". International Journal of Oncology 46.5 (2015): 2067-2075.
Chicago
Kim, S. W., Kim, S., Langley, R. R., Fidler, I. J."Modulation of the cancer cell transcriptome by culture media formulations and cell density". International Journal of Oncology 46, no. 5 (2015): 2067-2075. https://doi.org/10.3892/ijo.2015.2930
Copy and paste a formatted citation
x
Spandidos Publications style
Kim SW, Kim SJ, Langley RR and Fidler IJ: Modulation of the cancer cell transcriptome by culture media formulations and cell density. Int J Oncol 46: 2067-2075, 2015.
APA
Kim, S.W., Kim, S., Langley, R.R., & Fidler, I.J. (2015). Modulation of the cancer cell transcriptome by culture media formulations and cell density. International Journal of Oncology, 46, 2067-2075. https://doi.org/10.3892/ijo.2015.2930
MLA
Kim, S. W., Kim, S., Langley, R. R., Fidler, I. J."Modulation of the cancer cell transcriptome by culture media formulations and cell density". International Journal of Oncology 46.5 (2015): 2067-2075.
Chicago
Kim, S. W., Kim, S., Langley, R. R., Fidler, I. J."Modulation of the cancer cell transcriptome by culture media formulations and cell density". International Journal of Oncology 46, no. 5 (2015): 2067-2075. https://doi.org/10.3892/ijo.2015.2930
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