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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts

Corrigendum in: /10.3892/ijo.2022.5415
  • Authors:
    • Chao Li
    • Yan Li
    • Haining Lv
    • Shaowu Li
    • Ke Tang
    • Wanqi Zhou
    • Shishan Yu
    • Xiaoguang Chen
  • View Affiliations / Copyright

    Affiliations: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R. China, Department of Neurosurgery, Capital Medical University Affiliated Beijing Tiantan Hospital; Beijing Neurosurgical Institute, Beijing 100050, P.R. China
  • Pages: 179-187
    |
    Published online on: April 29, 2015
       https://doi.org/10.3892/ijo.2015.2977
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Abstract

Neuroblastoma is the most common cancer in infants and the fourth most common cancer in children. Our previous study showed that PF403 had a potent antitumor ability. In the present study, we evaluated the anti-neuroblastoma property of PF403 and investigated the underlying mechanisms. MTT assay, colony formation assay and flow cytometry assay were used to assess cytotoxicity of PF403 on SH-SY5Y cells. Transwell assay was chosen to estimate the anti-invasion ability of PF403 on neuroblastoma cells. The protein expression was detected by western blot analysis. The SH-SY5Y brain xenograft model was used to assess in vivo antitumor activity of PF403. PF403-mediated SH-SY5Y cell death was found to be dose- and time-dependent, and PF403 was able to limit invasion and metastasis of neuroblastoma cells. MRI and pathology analysis proved that the pro-drug of PF403, CAT3, inhibited SH-SY5Y cells in vivo. PF403 decreased expression of phosphorylated FAK, MMP-2 and MMP-9 proteins, and downregulated the activity of PI3K/AKT and Raf/ERK pathways, followed by regulation of the proteins expression of Bcl-2 family, activated caspase-3, -9 and PARP and initiation of apoptosis of neuroblastoma cells. PF403 exerted cytotoxicity against SH-SY5Y neuroblastoma cell both in vitro and in vivo, and inhibited its invasion ability, suggesting PF403 has potential as a new anticancer drug for the treatment of neuroblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Li C, Li Y, Lv H, Li S, Tang K, Zhou W, Yu S and Chen X: The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415. Int J Oncol 47: 179-187, 2015.
APA
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W. ... Chen, X. (2015). The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415. International Journal of Oncology, 47, 179-187. https://doi.org/10.3892/ijo.2015.2977
MLA
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W., Yu, S., Chen, X."The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415". International Journal of Oncology 47.1 (2015): 179-187.
Chicago
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W., Yu, S., Chen, X."The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415". International Journal of Oncology 47, no. 1 (2015): 179-187. https://doi.org/10.3892/ijo.2015.2977
Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Li Y, Lv H, Li S, Tang K, Zhou W, Yu S and Chen X: The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415. Int J Oncol 47: 179-187, 2015.
APA
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W. ... Chen, X. (2015). The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415. International Journal of Oncology, 47, 179-187. https://doi.org/10.3892/ijo.2015.2977
MLA
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W., Yu, S., Chen, X."The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415". International Journal of Oncology 47.1 (2015): 179-187.
Chicago
Li, C., Li, Y., Lv, H., Li, S., Tang, K., Zhou, W., Yu, S., Chen, X."The novel anti-neuroblastoma agent PF403, inhibits proliferation and invasion in vitro and in brain xenografts Corrigendum in /10.3892/ijo.2022.5415". International Journal of Oncology 47, no. 1 (2015): 179-187. https://doi.org/10.3892/ijo.2015.2977
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