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Article Open Access

Establishment of patient-derived cancer xenografts in immunodeficient NOG mice

  • Authors:
    • Tsuyoshi Chijiwa
    • Kenji Kawai
    • Akira Noguchi
    • Hidemitsu Sato
    • Akimune Hayashi
    • Haruhiko Cho
    • Manabu Shiozawa
    • Takeshi Kishida
    • Soichiro Morinaga
    • Tomoyuki Yokose
    • Makoto Katayama
    • Nobuo Takenaka
    • Hiroshi Suemizu
    • Roppei Yamada
    • Yoshiyasu Nakamura
    • Takashi Ohtsu
    • Yasuo Takano
    • Kohzoh Imai
    • Yohei Miyagi
    • Masato Nakamura
  • View Affiliations / Copyright

    Affiliations: Central Institute for Experimental Animals, Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan, Department of Pathology, Kanagawa Cancer Center, Nakao, Asahi-ku, Yokohama 241-8515, Japan, Department of Neurosurgery, Kanagawa Cancer Center, Nakao, Asahi-ku, Yokohama 241-8515, Japan, Department of Gastrointestinal Surgery, Kanagawa Cancer Center, Nakao, Asahi-ku, Yokohama 241-8515, Japan, Department of Urology, Kanagawa Cancer Center, Nakao, Asahi-ku, Yokohama 241-8515, Japan, Department of Neurosurgery, Kawasaki Municipal Hospital, Shinkawa-tori, Kawasaki 210-0013, Japan, Research Institute, Kanagawa Cancer Center, Nakao, Asahi-ku, Yokohama 241-8515, Japan
    Copyright: © Chijiwa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 61-70
    |
    Published online on: May 11, 2015
       https://doi.org/10.3892/ijo.2015.2997
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Abstract

Viable and stable human cancer cell lines and animal models combined with adequate clinical information are essential for future advances in cancer research and patient care. Conventional in vitro cancer cell lines are commonly available; however, they lack detailed information on the patient from which they originate, including disease phenotype and drug sensitivity. Patient-derived xenografts (PDX) with clinical information (so-called ‘cancer xenopatients’) are a promising advance that may accelerate the development of anticancer therapies. We established 61 PDX lines from 116 surgically removed tumor tissues inoculated subcutaneously into NOG mice (53% success rate). PDX lines were established from various types of epithelial tumors and also from sarcomas, including gastrointestinal stromal tumors and Ewing/PNET sarcomas. The metastatic tumors yielded PDX lines more effectively (65%) than the primary tumors (27%, P<0.001). In our PDX models, morphological characteristics, gene expression profiles, and genetic alteration patterns were all well preserved. In eight cases (7%), the transplantable xenografts for several generations were composed of large monotonous nonepithelial cells of human origin, revealed to be Epstein-Barr virus infection-associated lympho­proliferative lesions. Despite this, PDX linked with clinical information offer many advantages for preclinical studies investigating new anticancer drugs. The fast and efficient establishment of individual PDX may also contribute to future personalized anticancer therapies.
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View References

1 

Morton CL and Houghton PJ: Establishment of human tumor xenografts in immunodeficient mice. Nat Protoc. 2:247–250. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Jin K, Li G, Cui B, Zhang J, Lan H, Han N, Xie B, Cao F, He K, Wang H, et al: Assessment of a novel VEGF targeted agent using patient-derived tumor tissue xenograft models of colon carcinoma with lymphatic and hepatic metastases. PLoS One. 6:e283842011. View Article : Google Scholar : PubMed/NCBI

3 

Johnson JI, Decker S, Zaharevitz D, Rubinstein LV, Venditti JM, Schepartz S, Kalyandrug S, Christian M, Arbuck S, Hollingshead M, et al: Relationships between drug activity in NCI preclinical in vitro and in vivo models and early clinical trials. Br J Cancer. 84:1424–1431. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Sausville EA, Burger AM, Becher OJ and Holland EC: Contributions of human tumor xenografts to anticancer drug development. Cancer Res. 66:3351–3354. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Fujii E, Suzuki M, Matsubara K, Watanabe M, Chen YJ, Adachi K, Ohnishi Y, Tanigawa M, Tsuchiya M and Tamaoki N: Establishment and characterization of in vivo human tumor models in the NOD/SCID/gamma(c)(null) mouse. Pathol Int. 58:559–567. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Bertotti A, Migliardi G, Galimi F, Sassi F, Torti D, Isella C, Corà D, Di Nicolantonio F, Buscarino M, Petti C, et al: A molecularly annotated platform of patient-derived xenografts (‘xenopatients’) identifies HER2 as an effective therapeutic target in cetuximab-resistant colorectal cancer. Cancer Discov. 1:508–523. 2011. View Article : Google Scholar

7 

Galimi F, Torti D, Sassi F, Isella C, Corà D, Gastaldi S, Ribero D, Muratore A, Massucco P, Siatis D, et al: Genetic and expression analysis of MET, MACC1, and HGF in metastatic colorectal cancer: Response to met inhibition in patient xenografts and pathologic correlations. Clin Cancer Res. 17:3146–3156. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Kobayashi S, Yamada-Okabe H, Suzuki M, Natori O, Kato A, Matsubara K, Jau Chen Y, Yamazaki M, Funahashi S, Yoshida K, et al: LGR5-positive colon cancer stem cells inter-convert with drug-resistant LGR5-negative cells and are capable of tumor reconstitution. Stem Cells. 30:2631–2644. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Scott CL, Becker MA, Haluska P and Samimi G: Patient-derived xenograft models to improve targeted therapy in epithelial ovarian cancer treatment. Front Oncol. 3:2952013. View Article : Google Scholar : PubMed/NCBI

10 

Tokunaga T, Nakamura M, Oshika Y, Ohnishi Y and Ueyama Y: Is xenotransplantability of human colon cancers in SCID mice affected by angiogenic factors? J Natl Cancer Inst. 90:400–401. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Dangles-Marie V, Pocard M, Richon S, Weiswald LB, Assayag F, Saulnier P, Judde JG, Janneau JL, Auger N, Validire P, et al: Establishment of human colon cancer cell lines from fresh tumors versus xenografts: Comparison of success rate and cell line features. Cancer Res. 67:398–407. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, et al: NOD/SCID/gamma(c)(null) mouse: An excellent recipient mouse model for engraftment of human cells. Blood. 100:3175–3182. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Suemizu H, Monnai M, Ohnishi Y, Ito M, Tamaoki N and Nakamura M: Identification of a key molecular regulator of liver metastasis in human pancreatic carcinoma using a novel quantitative model of metastasis in NOD/SCID/gammacnull (NOG) mice. Int J Oncol. 31:741–751. 2007.PubMed/NCBI

14 

Chijiwa T, Abe Y, Ikoma N, Yamazaki H, Tsukamoto H, Suemizu H, Kawai K, Wakui M, Nishime C, Matsumoto H, et al: Thrombospondin 2 inhibits metastasis of human malignant melanoma through microenvironment-modification in NOD/SCID/gammaCnull (NOG) mice. Int J Oncol. 34:5–13. 2009.

15 

Kubo A, Ohmura M, Wakui M, Harada T, Kajihara S, Ogawa K, Suemizu H, Nakamura M, Setou M and Suematsu M: Semi-quantitative analyses of metabolic systems of human colon cancer metastatic xenografts in livers of superimmunodeficient NOG mice. Anal Bioanal Chem. 400:1895–1904. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Cho H, Watanabe T, Aoyama T, Hayashi T, Yamada T, Ogata T, Yoshikawa T, Tsuburaya A, Sekiguchi H, Nakamura Y, et al: Small bud of probable gastrointestinal stromal tumor within a laparoscopically-resected gastric schwannoma. Int J Clin Oncol. 17:294–298. 2012. View Article : Google Scholar

17 

Yoshida T, Kinoshita H, Segawa T, Nakamura E, Inoue T, Shimizu Y, Kamoto T and Ogawa O: Antiandrogen bicalutamide promotes tumor growth in a novel androgen-dependent prostate cancer xenograft model derived from a bicalutamide-treated patient. Cancer Res. 65:9611–9616. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Marangoni E, Vincent-Salomon A, Auger N, Degeorges A, Assayag F, de Cremoux P, de Plater L, Guyader C, De Pinieux G, Judde JG, et al: A new model of patient tumor-derived breast cancer xenografts for preclinical assays. Clin Cancer Res. 13:3989–3998. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Mueller BM and Reisfeld RA: Potential of the scid mouse as a host for human tumors. Cancer Metastasis Rev. 10:193–200. 1991. View Article : Google Scholar : PubMed/NCBI

20 

Bankert RB, Hess SD and Egilmez NK: SCID mouse models to study human cancer pathogenesis and approaches to therapy: Potential, limitations, and future directions. Front Biosci. 7:c44–c62. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Bibby MC: Orthotopic models of cancer for preclinical drug evaluation: Advantages and disadvantages. Eur J Cancer. 40:852–857. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Itoh T, Shiota M, Takanashi M, Hojo I, Satoh H, Matsuzawa A, Moriyama T, Watanabe T, Hirai K and Mori S: Engraftment of human non-Hodgkin lymphomas in mice with severe combined immunodeficiency. Cancer. 72:2686–2694. 1993. View Article : Google Scholar : PubMed/NCBI

23 

Fujii E, Kato A, Chen YJ, Matsubara K, Ohnishi Y and Suzuki M: Characterization of EBV-related lymphoproliferative lesions arising in donor lymphocytes of transplanted human tumor tissues in the NOG mouse. Exp Anim. 63:289–296. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Yokose T, et al: Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. Int J Oncol 47: 61-70, 2015.
APA
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H. ... Nakamura, M. (2015). Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. International Journal of Oncology, 47, 61-70. https://doi.org/10.3892/ijo.2015.2997
MLA
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H., Shiozawa, M., Kishida, T., Morinaga, S., Yokose, T., Katayama, M., Takenaka, N., Suemizu, H., Yamada, R., Nakamura, Y., Ohtsu, T., Takano, Y., Imai, K., Miyagi, Y., Nakamura, M."Establishment of patient-derived cancer xenografts in immunodeficient NOG mice". International Journal of Oncology 47.1 (2015): 61-70.
Chicago
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H., Shiozawa, M., Kishida, T., Morinaga, S., Yokose, T., Katayama, M., Takenaka, N., Suemizu, H., Yamada, R., Nakamura, Y., Ohtsu, T., Takano, Y., Imai, K., Miyagi, Y., Nakamura, M."Establishment of patient-derived cancer xenografts in immunodeficient NOG mice". International Journal of Oncology 47, no. 1 (2015): 61-70. https://doi.org/10.3892/ijo.2015.2997
Copy and paste a formatted citation
x
Spandidos Publications style
Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Yokose T, et al: Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. Int J Oncol 47: 61-70, 2015.
APA
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H. ... Nakamura, M. (2015). Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. International Journal of Oncology, 47, 61-70. https://doi.org/10.3892/ijo.2015.2997
MLA
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H., Shiozawa, M., Kishida, T., Morinaga, S., Yokose, T., Katayama, M., Takenaka, N., Suemizu, H., Yamada, R., Nakamura, Y., Ohtsu, T., Takano, Y., Imai, K., Miyagi, Y., Nakamura, M."Establishment of patient-derived cancer xenografts in immunodeficient NOG mice". International Journal of Oncology 47.1 (2015): 61-70.
Chicago
Chijiwa, T., Kawai, K., Noguchi, A., Sato, H., Hayashi, A., Cho, H., Shiozawa, M., Kishida, T., Morinaga, S., Yokose, T., Katayama, M., Takenaka, N., Suemizu, H., Yamada, R., Nakamura, Y., Ohtsu, T., Takano, Y., Imai, K., Miyagi, Y., Nakamura, M."Establishment of patient-derived cancer xenografts in immunodeficient NOG mice". International Journal of Oncology 47, no. 1 (2015): 61-70. https://doi.org/10.3892/ijo.2015.2997
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