Acquisition of new tumor cell properties by MSC-derived exosomes

  • Authors:
    • Yuanyuan Yang
    • Vesna Bucan
    • Heike Baehre
    • Juliane von der Ohe
    • Anna Otte
    • Ralf Hass
  • View Affiliations

  • Published online on: May 11, 2015     https://doi.org/10.3892/ijo.2015.3001
  • Pages: 244-252
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Abstract

Interaction between multi-functional mesenchymal stroma/stem cells (MSC) and human tumor cells involves the exchange of biological material via extracellular vesicles including exosomes. Protein analysis of MSC-derived exosomes demonstrated the presence of MMP-2 and MSC-specific markers including CD90 and ecto-5'-nucleotidase (CD73). Incubation of tumor cells with these membranous particles revealed a rapid uptake of MSC-released microvesicles whereby breast cancer cells incorporated ~19% and SCCOHT-1 cells representing a rare type of small cell ovarian cancer assimilated ~28% of available exosomes within 24 h. This interaction was accompanied by functional alterations of tumor cell properties during integration of exosomal content from MSC. Indeed, exosome-associated MMP-2 exhibited functional enzyme activity and MCF-7 breast cancer cells with undetectable MMP-2 protein acquired expression of this enzyme and corresponding gelatinase functionality after stimulation with MSC-derived exosomes. Similar effects were observed in SCCOHT-1 cells during culture in the presence of MSC-derived exosomes which enabled new metabolic activities in this tumor cell type. Together, these findings demonstrated that the internalization of MSC-derived exosomes was associated with the acquisition of new tumor cell properties by altering cellular functionalities and providing the capability to re-organize the tumor microenvironment.
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July-2015
Volume 47 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yang Y, Bucan V, Baehre H, von der Ohe J, Otte A and Hass R: Acquisition of new tumor cell properties by MSC-derived exosomes. Int J Oncol 47: 244-252, 2015
APA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., & Hass, R. (2015). Acquisition of new tumor cell properties by MSC-derived exosomes. International Journal of Oncology, 47, 244-252. https://doi.org/10.3892/ijo.2015.3001
MLA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47.1 (2015): 244-252.
Chicago
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47, no. 1 (2015): 244-252. https://doi.org/10.3892/ijo.2015.3001