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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

Acquisition of new tumor cell properties by MSC-derived exosomes

  • Authors:
    • Yuanyuan Yang
    • Vesna Bucan
    • Heike Baehre
    • Juliane von der Ohe
    • Anna Otte
    • Ralf Hass
  • View Affiliations / Copyright

    Affiliations: Biochemistry and Tumor Biology Laboratory, Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany, Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany, Institute of Pharmacology, Hannover Medical School, Hannover, Germany
  • Pages: 244-252
    |
    Published online on: May 11, 2015
       https://doi.org/10.3892/ijo.2015.3001
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Abstract

Interaction between multi-functional mesenchymal stroma/stem cells (MSC) and human tumor cells involves the exchange of biological material via extracellular vesicles including exosomes. Protein analysis of MSC-derived exosomes demonstrated the presence of MMP-2 and MSC-specific markers including CD90 and ecto-5'-nucleotidase (CD73). Incubation of tumor cells with these membranous particles revealed a rapid uptake of MSC-released microvesicles whereby breast cancer cells incorporated ~19% and SCCOHT-1 cells representing a rare type of small cell ovarian cancer assimilated ~28% of available exosomes within 24 h. This interaction was accompanied by functional alterations of tumor cell properties during integration of exosomal content from MSC. Indeed, exosome-associated MMP-2 exhibited functional enzyme activity and MCF-7 breast cancer cells with undetectable MMP-2 protein acquired expression of this enzyme and corresponding gelatinase functionality after stimulation with MSC-derived exosomes. Similar effects were observed in SCCOHT-1 cells during culture in the presence of MSC-derived exosomes which enabled new metabolic activities in this tumor cell type. Together, these findings demonstrated that the internalization of MSC-derived exosomes was associated with the acquisition of new tumor cell properties by altering cellular functionalities and providing the capability to re-organize the tumor microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Bucan V, Baehre H, von der Ohe J, Otte A and Hass R: Acquisition of new tumor cell properties by MSC-derived exosomes. Int J Oncol 47: 244-252, 2015.
APA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., & Hass, R. (2015). Acquisition of new tumor cell properties by MSC-derived exosomes. International Journal of Oncology, 47, 244-252. https://doi.org/10.3892/ijo.2015.3001
MLA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47.1 (2015): 244-252.
Chicago
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47, no. 1 (2015): 244-252. https://doi.org/10.3892/ijo.2015.3001
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Bucan V, Baehre H, von der Ohe J, Otte A and Hass R: Acquisition of new tumor cell properties by MSC-derived exosomes. Int J Oncol 47: 244-252, 2015.
APA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., & Hass, R. (2015). Acquisition of new tumor cell properties by MSC-derived exosomes. International Journal of Oncology, 47, 244-252. https://doi.org/10.3892/ijo.2015.3001
MLA
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47.1 (2015): 244-252.
Chicago
Yang, Y., Bucan, V., Baehre, H., von der Ohe, J., Otte, A., Hass, R."Acquisition of new tumor cell properties by MSC-derived exosomes". International Journal of Oncology 47, no. 1 (2015): 244-252. https://doi.org/10.3892/ijo.2015.3001
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