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Article

Constitutive expression of IRF-5 in HTLV-1-infected T cells

  • Authors:
    • Chie Ishikawa
    • Masachika Senba
    • Betsy J. Barnes
    • Naoki Mori
  • View Affiliations / Copyright

    Affiliations: Transdisciplinary Research Organization for Subtropics and Island Studies, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan, Department of Pathology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan, Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers Biomedical and Health Sciences, Newark, NJ 07103, USA, Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan
  • Pages: 361-369
    |
    Published online on: May 22, 2015
       https://doi.org/10.3892/ijo.2015.3020
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Abstract

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL), an aggressive and fatal leukemia of T cells. Interferon regulatory factor (IRF)-5 plays a critical role in the induction of interferon genes in viral infected cells. We examined the specific mechanisms underlying the expression and regulation of IRF-5 in HTLV-1-infected T cells. IRF-5 was constitutively transcribed into three distinct alternatively spliced isoforms (V1, V3 and V4) in HTLV-1-infected T-cell lines but not in uninfected T-cell lines. IRF-5 was also upregulated in HTLV-1-infected T-cell lines at protein level. Nuclear IRF-5 expression was noted in ATL cells present in lymph nodes and skin lesions. IRF-5 mRNA expression was induced following infection of T cells with HTLV-1, and specifically by viral oncoprotein Tax. Tax also activated V3 promoter. Microarray analysis of IRF-5-expressing uninfected T cells demonstrated that IRF-5 induced the expression of tumor necrosis factor family cytokines. The results suggest that IRF-5 is a Tax-regulated gene, and its expression may be associated with the pathogenesis of ATL.
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Copy and paste a formatted citation
Spandidos Publications style
Ishikawa C, Senba M, Barnes BJ and Mori N: Constitutive expression of IRF-5 in HTLV-1-infected T cells. Int J Oncol 47: 361-369, 2015.
APA
Ishikawa, C., Senba, M., Barnes, B.J., & Mori, N. (2015). Constitutive expression of IRF-5 in HTLV-1-infected T cells. International Journal of Oncology, 47, 361-369. https://doi.org/10.3892/ijo.2015.3020
MLA
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47.1 (2015): 361-369.
Chicago
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47, no. 1 (2015): 361-369. https://doi.org/10.3892/ijo.2015.3020
Copy and paste a formatted citation
x
Spandidos Publications style
Ishikawa C, Senba M, Barnes BJ and Mori N: Constitutive expression of IRF-5 in HTLV-1-infected T cells. Int J Oncol 47: 361-369, 2015.
APA
Ishikawa, C., Senba, M., Barnes, B.J., & Mori, N. (2015). Constitutive expression of IRF-5 in HTLV-1-infected T cells. International Journal of Oncology, 47, 361-369. https://doi.org/10.3892/ijo.2015.3020
MLA
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47.1 (2015): 361-369.
Chicago
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47, no. 1 (2015): 361-369. https://doi.org/10.3892/ijo.2015.3020
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