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Article

Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer

  • Authors:
    • Katsuhiro Uzawa
    • Atsushi Kasamatsu
    • Takao Baba
    • Yasushi Kimura
    • Dai Nakashima
    • Morihiro Higo
    • Yosuke Sakamoto
    • Katsunori Ogawara
    • Masashi Shiiba
    • Hideki Tanzawa
  • View Affiliations / Copyright

    Affiliations: Department of Oral Science, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260‑8670, Japan, Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba University, Chuo-ku, Chiba 260‑8670, Japan, Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260‑8670, Japan
  • Pages: 1077-1083
    |
    Published online on: July 15, 2015
       https://doi.org/10.3892/ijo.2015.3083
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Abstract

Circulating tumor cells (CTCs) and/or their relating molecules are promising determinants during the course of cancer treatment, especially for post-therapeutic monitoring. We recently reported the clinical relevance of detecting circulating tumor-associated mutant mitochondrial DNAs (mut-mtDNAs) at three different regions including the displacement loop, 12S-rRNA and 16S-rRNA in oral squamous cell carcinomas (OSCCs). In the present study, to further investigate if the other mut-mtDNAs have novel efficiency for detecting potential tumoral micrometastasis, mut-mtDNAs on the ND2 and ND3 regions of the genome in 240 clinical samples from patients with OSCC were assessed in vitro and in vivo by quantitative real-time PCR combined with high-resolution melting curve analysis. Furthermore, the clinical relevance was evaluated by the area under the receiver operating characteristic curve (AUC) analysis. Three discrete sequence variations were identified in OSCC derived cell lines at the regions of ND2 (T:A to C:G at position 5108) and ND3 (A:T to G:C at position 10397 and C:G to T:A at position 10400), whereas no mutation was observed in normal control human normal oral keratinocytes. In OSCC patients examined, the presence of mut-mtDNAs in serum during the postoperative period accurately predicted poor prognoses (ND2 AUC, 0.761; ND3 AUC, 0.704). The data presented here provide a novel approach for detecting the circulating mut-mtDNAs that are promising molecular markers for evaluating tumoral micrometastasis in OSCCs.
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Copy and paste a formatted citation
Spandidos Publications style
Uzawa K, Kasamatsu A, Baba T, Kimura Y, Nakashima D, Higo M, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Tanzawa H, et al: Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer. Int J Oncol 47: 1077-1083, 2015.
APA
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M. ... Tanzawa, H. (2015). Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer. International Journal of Oncology, 47, 1077-1083. https://doi.org/10.3892/ijo.2015.3083
MLA
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M., Sakamoto, Y., Ogawara, K., Shiiba, M., Tanzawa, H."Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer". International Journal of Oncology 47.3 (2015): 1077-1083.
Chicago
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M., Sakamoto, Y., Ogawara, K., Shiiba, M., Tanzawa, H."Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer". International Journal of Oncology 47, no. 3 (2015): 1077-1083. https://doi.org/10.3892/ijo.2015.3083
Copy and paste a formatted citation
x
Spandidos Publications style
Uzawa K, Kasamatsu A, Baba T, Kimura Y, Nakashima D, Higo M, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Tanzawa H, et al: Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer. Int J Oncol 47: 1077-1083, 2015.
APA
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M. ... Tanzawa, H. (2015). Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer. International Journal of Oncology, 47, 1077-1083. https://doi.org/10.3892/ijo.2015.3083
MLA
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M., Sakamoto, Y., Ogawara, K., Shiiba, M., Tanzawa, H."Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer". International Journal of Oncology 47.3 (2015): 1077-1083.
Chicago
Uzawa, K., Kasamatsu, A., Baba, T., Kimura, Y., Nakashima, D., Higo, M., Sakamoto, Y., Ogawara, K., Shiiba, M., Tanzawa, H."Quantitative detection of circulating tumor-derived mitochondrial NADH subunit variants as a potential prognostic biomarker for oral cancer". International Journal of Oncology 47, no. 3 (2015): 1077-1083. https://doi.org/10.3892/ijo.2015.3083
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