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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September-2015 Volume 47 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway

  • Authors:
    • Li Yan
    • Xiaolin Liu
    • Aijun Yin
    • Yuyan Wei
    • Qifeng Yang
    • Beihua Kong
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Ji'nan, Shandong 250012, P.R. China, Department of General Surgery, Qilu Hospital, Shandong University, Ji'nan, Shandong 250012, P.R. China
  • Pages: 1054-1060
    |
    Published online on: July 20, 2015
       https://doi.org/10.3892/ijo.2015.3094
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Abstract

Although the anticancer effects of Huaier extract have been widely investigated, including anti-proliferate, anti-angiogenic and anti-metastatic activities, the mechanisms are not well understood. This study aimed to elucidate the inhibitory effect of Huaier extract on tumor growth in cervical cancer cells and its molecular mechanisms. Cell viability and motility were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony assays, migration, and invasive assays, respectively. The distribution of the cell cycle was analyzed by flow cytometry. Huaier inhibited cell viability of SiHa and C33A cells in a time- and dose-dependent manner; cell migration and invasiveness were also suppressed; Huaier was able to cause G2/M cell cycle arrest in C33A cells. The western blot results confirmed Huaier dose-dependently increased expression of phosphorylated c-Jun N-terminal kinase (JNK), p-38 and downregulated the expression of phosphorylated extracellular signal-regulated kinase (ERK) in a time- and dose-dependently manner. In vivo experiments showed that Huaier significantly suppressed the tumor volume of SiHa cell xenografts. These data suggest that Huaier may inhibit tumor proliferation in cervical cancer via the JNK/p38 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yan L, Liu X, Yin A, Wei Y, Yang Q and Kong B: Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway. Int J Oncol 47: 1054-1060, 2015.
APA
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., & Kong, B. (2015). Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway. International Journal of Oncology, 47, 1054-1060. https://doi.org/10.3892/ijo.2015.3094
MLA
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., Kong, B."Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway". International Journal of Oncology 47.3 (2015): 1054-1060.
Chicago
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., Kong, B."Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway". International Journal of Oncology 47, no. 3 (2015): 1054-1060. https://doi.org/10.3892/ijo.2015.3094
Copy and paste a formatted citation
x
Spandidos Publications style
Yan L, Liu X, Yin A, Wei Y, Yang Q and Kong B: Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway. Int J Oncol 47: 1054-1060, 2015.
APA
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., & Kong, B. (2015). Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway. International Journal of Oncology, 47, 1054-1060. https://doi.org/10.3892/ijo.2015.3094
MLA
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., Kong, B."Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway". International Journal of Oncology 47.3 (2015): 1054-1060.
Chicago
Yan, L., Liu, X., Yin, A., Wei, Y., Yang, Q., Kong, B."Huaier aqueous extract inhibits cervical cancer cell proliferation via JNK/p38 pathway". International Journal of Oncology 47, no. 3 (2015): 1054-1060. https://doi.org/10.3892/ijo.2015.3094
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