Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer

  • Authors:
    • Beibei Ni
    • Xihu Yu
    • Xiaoyan Guo
    • Xinjuan Fan
    • Zihuan Yang
    • Peihuang Wu
    • Zixu Yuan
    • Yanhong Deng
    • Jianping Wang
    • Dianke Chen
    • Lei Wang
  • View Affiliations

  • Published online on: July 29, 2015     https://doi.org/10.3892/ijo.2015.3109
  • Pages: 1329-1338
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Abstract

Long non-coding RNA, urothelial cancer associated 1 (UCA1), is reported to play a critical role in progression of carcinogenesis. In the present study, we identified differential expression of UCA1 in colorectal cancer (CRC) and paired peritumoral tissues using gene expression microarray analyses. qPCR analysis confirmed that UCA1 was upregulated in CRC (p<0.001) and the expression of UCA1 was statistically correlated with lymph node metastasis (P=0.040), distant metastasis (P=0.043) and tumor stage (P=0.010). Kaplan-Meier analysis indicated that patients with high UCA1 expression had a poor prognosis. Moreover, multivariate analysis identified UCA1 overexpression as an independent predictor for CRC. We also found that knockdown of UCA1 significantly suppressed cell proliferation and metastasis in CRC cells. Flow cytometry assays showed UCA1 silencing induced G0/G1 growth arrest and apoptosis of CRC cells. To further investigate the regulatory mechanisms of UCA1, we identified that Ets-2 bound to the UCA1 core promoter using luciferase assays. Collectively, our findings suggested that UCA1 might be an important prognostic indicator in CRC and may be a potential target for diagnosis and gene therapy.
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October-2015
Volume 47 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ni B, Yu X, Guo X, Fan X, Yang Z, Wu P, Yuan Z, Deng Y, Wang J, Chen D, Chen D, et al: Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer. Int J Oncol 47: 1329-1338, 2015
APA
Ni, B., Yu, X., Guo, X., Fan, X., Yang, Z., Wu, P. ... Wang, L. (2015). Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer. International Journal of Oncology, 47, 1329-1338. https://doi.org/10.3892/ijo.2015.3109
MLA
Ni, B., Yu, X., Guo, X., Fan, X., Yang, Z., Wu, P., Yuan, Z., Deng, Y., Wang, J., Chen, D., Wang, L."Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer". International Journal of Oncology 47.4 (2015): 1329-1338.
Chicago
Ni, B., Yu, X., Guo, X., Fan, X., Yang, Z., Wu, P., Yuan, Z., Deng, Y., Wang, J., Chen, D., Wang, L."Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer". International Journal of Oncology 47, no. 4 (2015): 1329-1338. https://doi.org/10.3892/ijo.2015.3109