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Article

Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications

  • Authors:
    • Change Gao
    • Min Hong
    • Jiwei Geng
    • Huahua Zhou
    • Jian Dong
  • View Affiliations / Copyright

    Affiliations: Medical Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China, Medical Oncology, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650018, P.R. China
  • Pages: 1371-1378
    |
    Published online on: August 31, 2015
       https://doi.org/10.3892/ijo.2015.3140
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Abstract

Gene therapy is one of the most important aspects of molecular targeted therapeutic approaches for tumors. A small molecule targeting carrier plays an important role in this process. PI, a new peptide found in our phage library, has been specifically suggested, combined with the human triple-negative breast cancer cell line MDA-MB‑231, and may be developed as a targeting/individualization therapy strategy to be applied in breast cancer research. In this study, we further investigated whether this peptide could carry exogenous protein to the target cells by forming a fusion peptide. PI-GST and PI-TK were cloned into plasmids and used for expression studies, analyses of PI-mediated protein delivery efficiency, and to investigations into the effect of PI on thymidine kinase/ganciclovir-mediated cytotoxicity. Biodistribution profiles were also investigated in vivo. The results showed the PI fusion protein was expressed correctly in vitro, and could carry GST into the target cells. Under certain conditions, PI-TK sensitizes cells to ganciclovir more efficiently than TK. In vivo there was a trend for increased inhibition of tumor growth with PI-TK when ganciclovir was present. Therefore, our results suggest the potential of PI as a new specific target carrier in breast cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Gao C, Hong M, Geng J, Zhou H and Dong J: Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications. Int J Oncol 47: 1371-1378, 2015.
APA
Gao, C., Hong, M., Geng, J., Zhou, H., & Dong, J. (2015). Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications. International Journal of Oncology, 47, 1371-1378. https://doi.org/10.3892/ijo.2015.3140
MLA
Gao, C., Hong, M., Geng, J., Zhou, H., Dong, J."Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications". International Journal of Oncology 47.4 (2015): 1371-1378.
Chicago
Gao, C., Hong, M., Geng, J., Zhou, H., Dong, J."Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications". International Journal of Oncology 47, no. 4 (2015): 1371-1378. https://doi.org/10.3892/ijo.2015.3140
Copy and paste a formatted citation
x
Spandidos Publications style
Gao C, Hong M, Geng J, Zhou H and Dong J: Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications. Int J Oncol 47: 1371-1378, 2015.
APA
Gao, C., Hong, M., Geng, J., Zhou, H., & Dong, J. (2015). Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications. International Journal of Oncology, 47, 1371-1378. https://doi.org/10.3892/ijo.2015.3140
MLA
Gao, C., Hong, M., Geng, J., Zhou, H., Dong, J."Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications". International Journal of Oncology 47.4 (2015): 1371-1378.
Chicago
Gao, C., Hong, M., Geng, J., Zhou, H., Dong, J."Characterization of PI (breast cancer cell special peptide) in MDA-MB-231 breast cancer cells and its potential therapeutic applications". International Journal of Oncology 47, no. 4 (2015): 1371-1378. https://doi.org/10.3892/ijo.2015.3140
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