A quantitative proteomics study on olfactomedin 4 in the development of gastric cancer

  • Authors:
    • Xiaoping Ran
    • Xiaoming Xu
    • Yixuan Yang
    • Sha She
    • Min Yang
    • Shiying Li
    • Hong Peng
    • Xiangchun Ding
    • Huaidong Hu
    • Peng Hu
    • Dazhi Zhang
    • Hong Ren
    • Ligang Wu
    • Weiqun Zeng
  • View Affiliations

  • Published online on: September 16, 2015     https://doi.org/10.3892/ijo.2015.3168
  • Pages: 1932-1944
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Abstract

Gastric cancer (GC) is now one of the most common malignancies with a relatively high incidence and high mortality rate. The prognosis is closely related to the degree of tumor metastasis. The mechanism of metastasis is still unclear. Proteomics analysis is a powerful tool to study and evaluate protein expression in tumor tissues. In the present study, we collected 15 gastric cancer and adjacent normal gastric tissues and used the isobaric tags for relative and absolute quantitation (iTRAQ) method to identify differentially expressed proteins. A total of 134 proteins were differentially expressed between the cancerous and non-cancerous samples. Azurocidin 1 (AZU1), CPVL, olfactomedin 4 (OLFM4) and Villin 1 (VIL1) were upregulated and confirmed by western blot analysis, real-time quantitative PCR and immunohistochemical analyses. These results were in accordance with iTRAQ. Furthermore, silencing the OLFM4 expression suppressed the migration, invasion and proliferation of the GC cells in vitro. The present study represents a successful application of the iTRAQ method in analyzing the expression levels of thousands of proteins. Overexpression of OLFM4 in gastric cancer may induce the development of gastric cancer. Overall, suppression of OLFM4 expression may be a promising strategy in the development of novel cancer therapeutic drugs.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ran X, Xu X, Yang Y, She S, Yang M, Li S, Peng H, Ding X, Hu H, Hu P, Hu P, et al: A quantitative proteomics study on olfactomedin 4 in the development of gastric cancer. Int J Oncol 47: 1932-1944, 2015
APA
Ran, X., Xu, X., Yang, Y., She, S., Yang, M., Li, S. ... Zeng, W. (2015). A quantitative proteomics study on olfactomedin 4 in the development of gastric cancer. International Journal of Oncology, 47, 1932-1944. https://doi.org/10.3892/ijo.2015.3168
MLA
Ran, X., Xu, X., Yang, Y., She, S., Yang, M., Li, S., Peng, H., Ding, X., Hu, H., Hu, P., Zhang, D., Ren, H., Wu, L., Zeng, W."A quantitative proteomics study on olfactomedin 4 in the development of gastric cancer". International Journal of Oncology 47.5 (2015): 1932-1944.
Chicago
Ran, X., Xu, X., Yang, Y., She, S., Yang, M., Li, S., Peng, H., Ding, X., Hu, H., Hu, P., Zhang, D., Ren, H., Wu, L., Zeng, W."A quantitative proteomics study on olfactomedin 4 in the development of gastric cancer". International Journal of Oncology 47, no. 5 (2015): 1932-1944. https://doi.org/10.3892/ijo.2015.3168