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Article

Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells

  • Authors:
    • Xiyan Mu
    • Yaxiong Sang
    • Chunju Fang
    • Bin Shao
    • Lu Yang
    • Kui Yao
    • Xitong Zhao
    • Jinhai Gou
    • Yuquan Wei
    • Tao Yi
    • Yang Wu
    • Xia Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1901-1911
    |
    Published online on: September 21, 2015
       https://doi.org/10.3892/ijo.2015.3175
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Abstract

Human umbilical endothelial cells (HUVECs) have been proven to be effective in tumor anti-angiogenesis but the mechanism remained to be further demonstrated. The restricted ability of HUVECs to proliferate in vitro also limits their application on a large scale. In the present study, we immortalized HUVECs with hTERT genes by lentiviral infection and explored the antitumor immunity of hTERT-expressing HUVECs (HUVEC-TERTs). Results showed that HUVEC-TERTs maintained high telomere activity and expressed CD31, VEGFR-II and integrin α5. Passage-30 HUVEC-TERTs were able to form vascular tubes in vitro without showing signs of senescence. In vivo HUVEC-TERTs elicited antitumor immunity in mouse LL2 and CT26 models protectively and therapeutically. Both humoral and cellular immunity participated in the tumor anti-angiogenesis as HUVEC-neutralizing sera antibodies and HUVEC-specific CTL were detected. The subsets of activated spleen T lymphocytes included both CD4+ T cells and CD8+ T cells. Moreover, MDSCs and Tregs were decreased while T lymphocytes were aggregated in the tumor microenvironment. Collectively, the present study is the first to confirm the antitumor immunity of hTERT-immortalized HUVECs. Both anti-angiogenesis and tumor microenvironmental regulation participated in the antitumor activity. Transducing hTERT genes might be a new strategy to allow HUVECs to be applied on a large scale in cancer immunotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Mu X, Sang Y, Fang C, Shao B, Yang L, Yao K, Zhao X, Gou J, Wei Y, Yi T, Yi T, et al: Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells. Int J Oncol 47: 1901-1911, 2015.
APA
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K. ... Zhao, X. (2015). Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells. International Journal of Oncology, 47, 1901-1911. https://doi.org/10.3892/ijo.2015.3175
MLA
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K., Zhao, X., Gou, J., Wei, Y., Yi, T., Wu, Y., Zhao, X."Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells". International Journal of Oncology 47.5 (2015): 1901-1911.
Chicago
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K., Zhao, X., Gou, J., Wei, Y., Yi, T., Wu, Y., Zhao, X."Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells". International Journal of Oncology 47, no. 5 (2015): 1901-1911. https://doi.org/10.3892/ijo.2015.3175
Copy and paste a formatted citation
x
Spandidos Publications style
Mu X, Sang Y, Fang C, Shao B, Yang L, Yao K, Zhao X, Gou J, Wei Y, Yi T, Yi T, et al: Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells. Int J Oncol 47: 1901-1911, 2015.
APA
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K. ... Zhao, X. (2015). Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells. International Journal of Oncology, 47, 1901-1911. https://doi.org/10.3892/ijo.2015.3175
MLA
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K., Zhao, X., Gou, J., Wei, Y., Yi, T., Wu, Y., Zhao, X."Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells". International Journal of Oncology 47.5 (2015): 1901-1911.
Chicago
Mu, X., Sang, Y., Fang, C., Shao, B., Yang, L., Yao, K., Zhao, X., Gou, J., Wei, Y., Yi, T., Wu, Y., Zhao, X."Immunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells". International Journal of Oncology 47, no. 5 (2015): 1901-1911. https://doi.org/10.3892/ijo.2015.3175
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