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Article

MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells

  • Authors:
    • Naotake Funamizu
    • Curtis R. Lacy
    • Minori Kamada
    • Katsuhiko Yanaga
    • Yoshinobu Manome
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan, Howard University School of Medicine, Washington, DC, USA, Department of Molecular Cell Biology, The Jikei University School of Medicine, Tokyo, Japan
  • Pages: 1981-1988
    |
    Published online on: September 22, 2015
       https://doi.org/10.3892/ijo.2015.3178
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Abstract

The present study investigated the relationship between microRNA-203 (miR-203) and the p53 upregulated modulator of apoptosis (Puma) in colon (HCT116) and lung cancer (A549) cells. Colon and lung cancer cell lines were selected for this study since a relationship between p53/miR-203 and p53/Puma has been established in both cancers. In the present study, adriamycin and nutlin-3 were used to activate p53, which induced both miR-203 and Puma expression in HCT116 cells. In contrast, HCT 116 cells with downregulated p53 showed decreased miR-203 and Puma expression. Importantly, we found that overexpressed miR-203 in HCT116 cells resulted in significantly increased Puma expression (P<0.05). Based on these findings, we hypothesized that another limb of the p53/Puma axis depends on miR-203 expression. To further validate this relationship, we used lung cancer cells (A549) and found that activated p53 increased both miR-203 and Puma expression. In addition, we found that Puma expression remained elevated in cells with overexpressed miR-203 in the presence of p53 downregulation. Cumulatively, our data purport that p53 not only increased Puma expression directly, but that it may also do so through miR-203. Additionally, functional studies revealed that miR-203 overexpression induced apoptosis and inhibited cell invasiveness.
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Copy and paste a formatted citation
Spandidos Publications style
Funamizu N, Lacy CR, Kamada M, Yanaga K and Manome Y: MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. Int J Oncol 47: 1981-1988, 2015.
APA
Funamizu, N., Lacy, C.R., Kamada, M., Yanaga, K., & Manome, Y. (2015). MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. International Journal of Oncology, 47, 1981-1988. https://doi.org/10.3892/ijo.2015.3178
MLA
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47.5 (2015): 1981-1988.
Chicago
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47, no. 5 (2015): 1981-1988. https://doi.org/10.3892/ijo.2015.3178
Copy and paste a formatted citation
x
Spandidos Publications style
Funamizu N, Lacy CR, Kamada M, Yanaga K and Manome Y: MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. Int J Oncol 47: 1981-1988, 2015.
APA
Funamizu, N., Lacy, C.R., Kamada, M., Yanaga, K., & Manome, Y. (2015). MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells. International Journal of Oncology, 47, 1981-1988. https://doi.org/10.3892/ijo.2015.3178
MLA
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47.5 (2015): 1981-1988.
Chicago
Funamizu, N., Lacy, C. R., Kamada, M., Yanaga, K., Manome, Y."MicroRNA-203 induces apoptosis by upregulating Puma expression in colon and lung cancer cells". International Journal of Oncology 47, no. 5 (2015): 1981-1988. https://doi.org/10.3892/ijo.2015.3178
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