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Article

The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma

  • Authors:
    • Shiyuan Song
    • Ying Wang
    • Po Xu
    • Ruina Yang
    • Zhikun Ma
    • Shuo Liang
    • Guangping Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Τhe First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Department of Urology, Τhe First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
  • Pages: 1819-1828
    |
    Published online on: September 24, 2015
       https://doi.org/10.3892/ijo.2015.3182
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Abstract

Histone deacetylase 8 (HDAC8), a unique member of class I HDACs, shows remarkable correlation with advanced disease stage. The depletion of HDAC8 leads to inhibition of proliferation, apoptosis and cell cycle arrest in multiple malignant tumors. However, little is known about the contribution of HDAC8 to the tumorigenesis of gastric cancer (GC). The present study investigated expression of HDAC8 in GC cell lines and tissues, and the roles of HDAC8 inhibition in the proliferation, cell cycle and apoptosis of gastric cancer cells and explored the potential mechanisms. In the present study, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry were used to examine the mRNA and protein expression of HDAC8 in GC cell lines and tissues. Then, the correlation between the clinicopathological parameters and the expression of HDAC8 was assessed. Finally, siRNA transfection and HDAC8 plasmid was performed to explore the functions of HDAC8 in GC progression in vitro. We found that the expression of HDAC8 was significantly upregulated both in GC cell lines and tumor tissues compared to human normal gastric epithelial cell, GES-1 and matched non-tumor tissues. Furthermore, depletion of HDAC8 remarkably inhibited GC cell proliferation, increased the apoptosis rate and G0/G1 phase percentage in vitro. Western blotting showed that the expression of protein promoting apoptosis such as, Bmf, activated caspase-3, caspase-6 were elevated following HDAC8 depletion. Our data exhibited an important role of HDAC8 in promoting gastric cancer tumorigenesis and identify this HDAC8 as a potential therapeutic target for the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Song S, Wang Y, Xu P, Yang R, Ma Z, Liang S and Zhang G: The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47: 1819-1828, 2015.
APA
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., & Zhang, G. (2015). The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. International Journal of Oncology, 47, 1819-1828. https://doi.org/10.3892/ijo.2015.3182
MLA
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., Zhang, G."The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma". International Journal of Oncology 47.5 (2015): 1819-1828.
Chicago
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., Zhang, G."The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma". International Journal of Oncology 47, no. 5 (2015): 1819-1828. https://doi.org/10.3892/ijo.2015.3182
Copy and paste a formatted citation
x
Spandidos Publications style
Song S, Wang Y, Xu P, Yang R, Ma Z, Liang S and Zhang G: The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47: 1819-1828, 2015.
APA
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., & Zhang, G. (2015). The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. International Journal of Oncology, 47, 1819-1828. https://doi.org/10.3892/ijo.2015.3182
MLA
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., Zhang, G."The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma". International Journal of Oncology 47.5 (2015): 1819-1828.
Chicago
Song, S., Wang, Y., Xu, P., Yang, R., Ma, Z., Liang, S., Zhang, G."The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma". International Journal of Oncology 47, no. 5 (2015): 1819-1828. https://doi.org/10.3892/ijo.2015.3182
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