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Article

Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications

  • Authors:
    • Agata Poniewierska-Baran
    • Malwina Suszynska
    • Wenyue Sun
    • Ahmed Abdelbaset-Ismail
    • Gabriela Schneider
    • Frederic G. Barr
    • Mariusz Z. Ratajczak
  • View Affiliations / Copyright

    Affiliations: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA, Department of Regenerative Medicine, Warsaw Medical University, Warsaw, Poland, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA
  • Pages: 1989-1997
    |
    Published online on: September 24, 2015
       https://doi.org/10.3892/ijo.2015.3184
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Abstract

The erythropoietin receptor (EpoR) is expressed by cells from the erythroid lineage; however, evidence has accumulated that it is also expressed by some solid tumors. This is an important observation, because recombinant erythropoietin (EPO) is employed in cancer patients to treat anemia related to chemo/radiotherapy. In our studies we employed eight rhabdomyosarcoma (RMS) cell lines (three alveolar-type RMS cell lines and five embrional‑type RMS cell lines), and mRNA samples obtained from positive, PAX7-FOXO1‑positive, and fusion-negative RMS patient samples. Expression of EpoR was evaluated by RT-PCR, gene array and FACS. The functionality of EpoR in RMS cell lines was evaluated by chemotaxis, adhesion, and direct cell proliferation assays. In some of the experiments, RMS cells were exposed to vincristine (VCR) in the presence or absence of EPO to test whether EPO may impair the therapeutic effect of VCR. We report for a first time that functional EpoR is expressed in human RMS cell lines as well as by primary tumors from RMS patients. Furthermore, EpoR is detectably expressed in both embryonal and alveolar RMS subtypes. At the functional level, several human RMS cell lines responded to EPO stimulation by enhanced proliferation, chemotaxis, cell adhesion, and phosphorylation of MAPKp42/44 and AKT. Moreover, RMS cells became more resistant to VCR treatment in the presence of EPO. Our findings have important potential clinical implications, indicating that EPO supplementation in RMS patients may have the unwanted side effect of tumor progression.
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Copy and paste a formatted citation
Spandidos Publications style
Poniewierska-Baran A, Suszynska M, Sun W, Abdelbaset-Ismail A, Schneider G, Barr FG and Ratajczak MZ: Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications. Int J Oncol 47: 1989-1997, 2015.
APA
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F.G., & Ratajczak, M.Z. (2015). Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications. International Journal of Oncology, 47, 1989-1997. https://doi.org/10.3892/ijo.2015.3184
MLA
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F. G., Ratajczak, M. Z."Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications". International Journal of Oncology 47.5 (2015): 1989-1997.
Chicago
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F. G., Ratajczak, M. Z."Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications". International Journal of Oncology 47, no. 5 (2015): 1989-1997. https://doi.org/10.3892/ijo.2015.3184
Copy and paste a formatted citation
x
Spandidos Publications style
Poniewierska-Baran A, Suszynska M, Sun W, Abdelbaset-Ismail A, Schneider G, Barr FG and Ratajczak MZ: Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications. Int J Oncol 47: 1989-1997, 2015.
APA
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F.G., & Ratajczak, M.Z. (2015). Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications. International Journal of Oncology, 47, 1989-1997. https://doi.org/10.3892/ijo.2015.3184
MLA
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F. G., Ratajczak, M. Z."Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications". International Journal of Oncology 47.5 (2015): 1989-1997.
Chicago
Poniewierska-Baran, A., Suszynska, M., Sun, W., Abdelbaset-Ismail, A., Schneider, G., Barr, F. G., Ratajczak, M. Z."Human rhabdomyosarcoma cells express functional erythropoietin receptor: Potential therapeutic implications". International Journal of Oncology 47, no. 5 (2015): 1989-1997. https://doi.org/10.3892/ijo.2015.3184
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