Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide

  • Authors:
    • Pengcheng He
    • Yanfeng Liu
    • Jun Qi
    • Huachao Zhu
    • Yuan Wang
    • Jing Zhao
    • Xiaoyan Cheng
    • Chen Wang
    • Mei Zhang
  • View Affiliations

  • Published online on: October 22, 2015     https://doi.org/10.3892/ijo.2015.3217
  • Pages: 2286-2295
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Arsenic sulfide (As4S4), an oral form of arsenic agent, has been shown to have similar efficacy and safety to intravenous arsenic trioxide in the treatment of acute promyelocytic leukemia (APL). The aim of the present study was to identify proteins modulated by As4S4 and to determine their involvement in the apoptotic pathway. We used comparative proteomic analysis to screen and identify the proteins that were differentially expressed with As4S4 treatment. Prohibitin (PHB) was selected for its diverse role and its increased expression in the cells treated with As4S4. To examine whether PHB play a functional role, two clones of PHB-knockdown and PHB-overexpression were generated by transfection of NB4-R1 with vectors containing PHB gene sequences. In comparison with parental NB4-R1 cells, PHB overexpression showed an increase in baseline apoptosis and an enhanced response in As4S4-induced apoptosis. PML-RARα fusion protein was found to be reduced with PHB-overexpression, and following As4S4 treatment, a greater reduction of promyelocytic leukemia-retinoic acid receptor-α (PML-RARα) fusion protein was seen in PHB-overexpression than that in parental cells. Consistently, PHB knockdown presented with a significant reduction in As4S4-induced apoptosis and a lesser degree of PML-RARα degradation. The results indicate the antitumor activity of PHB in promoting apoptosis of APL cells.
View Figures
View References

Related Articles

Journal Cover

December-2015
Volume 47 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
He P, Liu Y, Qi J, Zhu H, Wang Y, Zhao J, Cheng X, Wang C and Zhang M: Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide. Int J Oncol 47: 2286-2295, 2015
APA
He, P., Liu, Y., Qi, J., Zhu, H., Wang, Y., Zhao, J. ... Zhang, M. (2015). Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide. International Journal of Oncology, 47, 2286-2295. https://doi.org/10.3892/ijo.2015.3217
MLA
He, P., Liu, Y., Qi, J., Zhu, H., Wang, Y., Zhao, J., Cheng, X., Wang, C., Zhang, M."Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide". International Journal of Oncology 47.6 (2015): 2286-2295.
Chicago
He, P., Liu, Y., Qi, J., Zhu, H., Wang, Y., Zhao, J., Cheng, X., Wang, C., Zhang, M."Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide". International Journal of Oncology 47, no. 6 (2015): 2286-2295. https://doi.org/10.3892/ijo.2015.3217