Involvement of cyclic-nucleotide response element-binding family members in the radiation response of Ramos B lymphoma cells

Di Nisio,Chiara; Sancilio,Silvia; Di Giacomo,Viviana; Rapino,Monica; Sancillo,Laura; Genovesi,Domenico; Di Siena,Alessandro; Rana,Rosa Alba; Cataldi,Amelia; Di Pietro,Roberta

doi:10.3892/ijo.2015.3238

Involvement of cyclic-nucleotide response element-binding family members in the radiation response of Ramos B lymphoma cells

Authors:
- Chiara Di Nisio
- Silvia Sancilio
- Viviana Di Giacomo
- Monica Rapino
- Laura Sancillo
- Domenico Genovesi
- Alessandro Di Siena
- Rosa Alba Rana
- Amelia Cataldi
- Roberta Di Pietro
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Affiliations: Department of Pharmacy, G. d'Annunzio University, I-66100 Chieti, Italy, Molecular Genetics Institute CNR, I-27100 Pavia, Italy, Department of Medicine and Ageing Sciences, G. d'Annunzio University, I-66100 Chieti, Italy, Institute of Oncologic Radiotherapy, G. d'Annunzio University, I-66100 Chieti, Italy, Sanitary Physics Unit, SS. Annunziata Hospital, I-66100 Chieti, Italy
Published online on: November 9, 2015 https://doi.org/10.3892/ijo.2015.3238
Pages: 28-36
Copyright: © Di Nisio et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the role of Cyclic-nucleotide Response Element-Binding (CREB) family members and related nuclear transcription factors in the radiation response of human B lymphoma cell lines (Daudi and Ramos). Unlike the more radiosensitive Daudi cells, Ramos cells demonstrated only a moderate increase in early apoptosis after 3-5 Gy irradiation doses, which was detected with Annexin V/PI staining. Moreover, a significant and dose-dependent G2/M phase accumulation was observed in the same cell line at 24 h after both ionizing radiation (IR) doses. Western blot analysis showed an early increase in CREB protein expression that was still present at 3 h and more evident after 3 Gy IR in Ramos cells, along with the dose-dependent upregulation of p53 and NF-κB. These findings were consistent with real-time RT-PCR analysis that showed an early- and dose-dependent upregulation of NFKB1, IKBKB and XIAP gene expression. Unexpectedly, pre-treatment with SN50 did not increase cell death, but cell viability. Taken together, these findings let us hypothesise that the early induction and activation of NF-κB1 in Ramos cells could mediate necrotic cell death and be linked to other molecules belonging to CREB family and involved in the cell cycle regulation.

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