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Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma

  • Authors:
    • Justyna Wojas-Turek
    • Agnieszka Szczygieł
    • Jagoda Kicielińska
    • Joanna Rossowska
    • Egbert Piasecki
    • Elżbieta Pajtasz-Piasecka
  • View Affiliations / Copyright

    Affiliations: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
    Copyright: © Wojas-Turek et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 493-505
    |
    Published online on: December 7, 2015
       https://doi.org/10.3892/ijo.2015.3278
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Abstract

The present study shows that an application of cyclophosphamide (CY) supported by dendritic cell (DC)-based vaccines affected differentiation of the activity of CD4+ T cell subpopulations accompanied by an alteration in CD8+ cell number. Vaccines were composed of bone marrow-derived DCs activated with tumor cell lysate (BM-DC/TAgTNF-α) and/or genetically modified DCs of JAWS II line (JAWS II/Neo or JAWS II/IL-2 cells). Compared to untreated or CY-treated mice, the combined treatment of MC38 colon carcinoma-bearing mice resulted in significant tumor growth inhibition associated with an increase in influx of CD4+ and CD8+ T cells into tumor tissue. Whereas, the division of these cell population in spleen was not observed. Depending on the nature of DC-based vaccines and number of their applications, both tumor infiltrating cells and spleen cells were able to produce various amount of IFN-γ, IL-4 and IL-10 after mitogenic ex vivo stimulation. The administration of CY followed by BM-DC/TAgTNF-α and genetically modified JAWS II cells, increased the percentage of CD4+T-bet+ and CD4+GATA3+ cells and decreased the percentage of CD4+RORγt+ and CD4+FoxP3+ lymphocytes. However, the most intensive response against tumor was noted after the ternary treatment with CY + BM-DC/TAgTNF-α + JAWS II/IL-2 cells. Thus, the administration of various DC-based vaccines was responsible for generation of the diversified antitumor response. These findings demonstrate that the determination of the size of particular CD4+ T cell subpopulations may become a prognostic factor and be the basis for future development of anticancer therapy.
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View References

1 

Ding ZC, Blazar BR, Mellor AL, Munn DH and Zhou G: Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype. Blood. 115:2397–2406. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Zhao J, Cao Y, Lei Z, Yang Z, Zhang B and Huang B: Selective depletion of CD4+CD25+Foxp3+ regulatory T cells by low-dose cyclophosphamide is explained by reduced intracellular ATP levels. Cancer Res. 70:4850–4858. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Sistigu A, Viaud S, Chaput N, Bracci L, Proietti E and Zitvogel L: Immunomodulatory effects of cyclophosphamide and implementations for vaccine design. Semin Immunopathol. 33:369–383. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Matar P, Rozados VR, Gervasoni SI and Scharovsky GO: Th2/ Th1 switch induced by a single low dose of cyclophosphamide in a rat metastatic lymphoma model. Cancer Immunol Immunother. 50:588–596. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Liu JY, Wu Y, Zhang XS, Yang JL, Li HL, Mao YQ, Wang Y, Cheng X, Li YQ, Xia JC, et al: Single administration of low dose cyclophosphamide augments the antitumor effect of dendritic cell vaccine. Cancer Immunol Immunother. 56:1597–1604. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Veltman JD, Lambers MEH, van Nimwegen M, de Jong S, Hendriks RW, Hoogsteden HC, Aerts JG and Hegmans JP: Low-dose cyclophosphamide synergizes with dendritic cell-based immunotherapy in antitumor activity. J Biomed Biotechnol. 2010:7984672010. View Article : Google Scholar : PubMed/NCBI

7 

Balkow S, Loser K, Krummen M, Higuchi T, Rothoeft T, Apelt J, Tuettenberg A, Weishaupt C, Beissert S and Grabbe S: Dendritic cell activation by combined exposure to anti-CD40 plus interleukin (IL)-12 and IL-18 efficiently stimulates anti-tumor immunity. Exp Dermatol. 18:78–87. 2009. View Article : Google Scholar

8 

Brunner C, Seiderer J, Schlamp A, Bidlingmaier M, Eigler A, Haimerl W, Lehr HA, Krieg AM, Hartmann G and Endres S: Enhanced dendritic cell maturation by TNF-alpha or cytidine-phosphate-guanosine DNA drives T cell activation in vitro and therapeutic anti-tumor immune responses in vivo. J Immunol. 165:6278–6286. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Rossowska J, Pajtasz-Piasecka E, Szyda A, Krawczenko A, Zietara N and Dus D: Tumour antigen-loaded mouse dendritic cells maturing in the presence of inflammatory cytokines are potent activators of immune response in vitro but not in vivo. Oncol Rep. 21:1539–1549. 2009.PubMed/NCBI

10 

Pajtasz-Piasecka E and Indrová M: Dendritic cell-based vaccines for the therapy of experimental tumors. Immunotherapy. 2:257–268. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Rossowska J, Pajtasz-Piasecka E, Ryśnik O, Wojas J, Krawczenko A, Szyda A and Duś D: Generation of antitumor response by IL-2-transduced JAWS II dendritic cells. Immunobiology. 216:1074–1084. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Rossowska J, Pajtasz-Piasecka E, Anger N, Wojas-Turek J, Kicielińska J, Piasecki E and Duś D: Cyclophosphamide and IL-12-transduced DCs enhance the antitumor activity of tumor antigen-stimulated DCs and reduce Tregs and MDSCs number. J Immunother. 37:427–439. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Steinman RM: Decisions about dendritic cells: Past, present, and future. Annu Rev Immunol. 30:1–22. 2012. View Article : Google Scholar

14 

Coquerelle C and Moser M: DC subsets in positive and negative regulation of immunity. Immunol Rev. 234:317–334. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Kalinski P, Urban J, Narang R, Berk E, Wieckowski E and Muthuswamy R: Dendritic cell-based therapeutic cancer vaccines: What we have and what we need. Future Oncol. 5:379–390. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Palucka K, Ueno H, Roberts L, Fay J and Banchereau J: Dendritic cell subsets as vectors and targets for improved cancer therapy. Curr Top Microbiol Immunol. 344:173–192. 2011.

17 

Fong L, Brockstedt D, Benike C, Breen JK, Strang G, Ruegg CL and Engleman EG: Dendritic cell-based xenoantigen vaccination for prostate cancer immunotherapy. J Immunol. 167:7150–7156. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Nencioni A, Grünebach F, Schmidt SM, Müller MR, Boy D, Patrone F, Ballestrero A and Brossart P: The use of dendritic cells in cancer immunotherapy. Crit Rev Oncol Hematol. 65:191–199. 2008. View Article : Google Scholar

19 

Kuchroo VK, Das MP, Brown JA, Ranger AM, Zamvil SS, Sobel RA, Weiner HL, Nabavi N and Glimcher LH: B7-1 and B7-2 costimulatory molecules activate differentially the Th1/Th2 developmental pathways: Application to autoimmune disease therapy. Cell. 80:707–718. 1995. View Article : Google Scholar : PubMed/NCBI

20 

Ranger AM, Das MP, Kuchroo VK, Glimcher LH and Ploegh H: B7-2 (CD86) is essential for the development of IL-4-producing T cells. Int Immunol. 8:1549–1560. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Wojas-Turek J, Pajtasz-Piasecka E, Rossowska J, Piasecki E and Duś D: Antitumor effect of murine dendritic and tumor cells transduced with IL-2 gene. Folia Histochem Cytobiol. 50:414–419. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Cannon MJ, Goyne H, Stone PJB and Chiriva-Internati M: Dendritic cell vaccination against ovarian cancer - tipping the Treg/TH17 balance to therapeutic advantage? Expert Opin Biol Ther. 11:441–445. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Galon J, Costes A, Sanchez-Cabo F, Kirilovsky A, Mlecnik B, Lagorce-Pagès C, Tosolini M, Camus M, Berger A, Wind P, et al: Type, density, and location of immune cells within human colorectal tumors predict clinical outcome. Science. 313:1960–1964. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Pajtasz-Piasecka E, Rossowska J, Szyda A, Krawczenko A and Dus D: Generation of anti-tumor response by JAWS II mouse dendritic cells transduced with murine interleukin 12 genes. Oncol Rep. 17:1249–1257. 2007.PubMed/NCBI

25 

Lança T and Silva-Santos B: The split nature of tumor-infiltrating leukocytes: Implications for cancer surveillance and immunotherapy. Oncoimmunology. 1:717–725. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Rossowska J, Anger N, Kicielińska J, Pajtasz-Piasecka E, Bielawska-Pohl A, Wojas-Turek J and Duś D: Temporary elimination of IL-10 enhanced the effectiveness of cyclophosphamide and BMDC-based therapy by decrease of the suppressor activity of MDSCs and activation of antitumour immune response. Immunobiology. 220:389–398. 2015. View Article : Google Scholar

27 

Escobar A, López M, Serrano A, Ramirez M, Pérez C, Aguirre A, González R, Alfaro J, Larrondo M, Fodor M, et al: Dendritic cell immunizations alone or combined with low doses of interleukin-2 induce specific immune responses in melanoma patients. Clin Exp Immunol. 142:555–568. 2005.PubMed/NCBI

28 

Matias BF, de Oliveira TM, Rodrigues CM, Abdalla DR, Montes L, Murta EFC and Michelin MA: Influence of immunotherapy with autologous dendritic cells on innate and adaptive immune response in cancer. Clin Med Insights Oncol. 7:165–172. 2013.PubMed/NCBI

29 

Yamamoto M, Kamigaki T, Yamashita K, Hori Y, Hasegawa H, Kuroda D, Moriyama H, Nagata M, Ku Y and Kuroda Y: Enhancement of anti-tumor immunity by high levels of Th1 and Th17 with a combination of dendritic cell fusion hybrids and regulatory T cell depletion in pancreatic cancer. Oncol Rep. 22:337–343. 2009.PubMed/NCBI

30 

Guo C, Manjili MH, Subjeck JR, Sarkar D, Fisher PB and Wang XY: Therapeutic cancer vaccines: Past, present, and future. Adv Cancer Res. 119:421–475. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Zhu J and Paul WE: Heterogeneity and plasticity of T helper cells. Cell Res. 20:4–12. 2010. View Article : Google Scholar

32 

Baek S, Kim CS, Kim SB, Kim YM, Kwon SW, Kim Y, Kim H and Lee H: Combination therapy of renal cell carcinoma or breast cancer patients with dendritic cell vaccine and IL-2: Results from a phase I/II trial. J Transl Med. 9:1782011. View Article : Google Scholar : PubMed/NCBI

33 

Saha A and Chatterjee SK: Combination of CTL-associated antigen-4 blockade and depletion of CD25 regulatory T cells enhance tumour immunity of dendritic cell-based vaccine in a mouse model of colon cancer. Scand J Immunol. 71:70–82. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Cope A, Le Friec G, Cardone J and Kemper C: The Th1 life cycle: Molecular control of IFN-γ to IL-10 switching. Trends Immunol. 32:278–286. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Kicielińska J and Pajtasz-Piasecka E: The role of IL-10 in the modulation of the immune response in normal conditions and the tumor environment. Postepy Hig Med Dosw Online. 68:879–892. 2014.(In Polish). View Article : Google Scholar

36 

Shoemaker J, Saraiva M and O'Garra A: GATA-3 directly remodels the IL-10 locus independently of IL-4 in CD4+ T cells. J Immunol. 176:3470–3479. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Koller FL, Hwang DG, Dozier EA and Fingleton B: Epithelial interleukin-4 receptor expression promotes colon tumor growth. Carcinogenesis. 31:1010–1017. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wojas-Turek J, Szczygieł A, Kicielińska J, Rossowska J, Piasecki E and Pajtasz-Piasecka E: Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma. Int J Oncol 48: 493-505, 2016.
APA
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., & Pajtasz-Piasecka, E. (2016). Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma. International Journal of Oncology, 48, 493-505. https://doi.org/10.3892/ijo.2015.3278
MLA
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., Pajtasz-Piasecka, E."Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma". International Journal of Oncology 48.2 (2016): 493-505.
Chicago
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., Pajtasz-Piasecka, E."Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma". International Journal of Oncology 48, no. 2 (2016): 493-505. https://doi.org/10.3892/ijo.2015.3278
Copy and paste a formatted citation
x
Spandidos Publications style
Wojas-Turek J, Szczygieł A, Kicielińska J, Rossowska J, Piasecki E and Pajtasz-Piasecka E: Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma. Int J Oncol 48: 493-505, 2016.
APA
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., & Pajtasz-Piasecka, E. (2016). Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma. International Journal of Oncology, 48, 493-505. https://doi.org/10.3892/ijo.2015.3278
MLA
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., Pajtasz-Piasecka, E."Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma". International Journal of Oncology 48.2 (2016): 493-505.
Chicago
Wojas-Turek, J., Szczygieł, A., Kicielińska, J., Rossowska, J., Piasecki, E., Pajtasz-Piasecka, E."Treatment with cyclophosphamide supported by various dendritic cell-based vaccines induces diversification in CD4+ T cell response against MC38 colon carcinoma". International Journal of Oncology 48, no. 2 (2016): 493-505. https://doi.org/10.3892/ijo.2015.3278
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