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Article

Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel

  • Authors:
    • Yi-Sheng Chou
    • Chueh-Chuan Yen
    • Wei-Ming Chen
    • Yung-Chan Lin
    • Yao-Shan Wen
    • Wei-Ting Ke
    • Jir-You Wang
    • Chun-Yu Liu
    • Muh-Hwa Yang
    • Tain-Hsiung Chen
    • Chien-Lin Liu
  • View Affiliations / Copyright

    Affiliations: Division of Medical Oncology, Department of Oncology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C., Therapeutical and Research Center of Musculoskeletal Tumor, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
  • Pages: 1187-1194
    |
    Published online on: January 20, 2016
       https://doi.org/10.3892/ijo.2016.3352
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Abstract

Polo-like kinase 1 (PLK1), a serine/threonine kinase and an oncogene, is crucial in regulating cell cycle progression. PLK1 also has been demonstrated as a potential target of osteosarcoma (OS) by using short hairpin RNA libraries in lentiviral vectors for screening of protein kinase. In preclinical studies, GSK461364, a potent and selective ATP-competitive PLK1 inhibitor, showed antiproliferative activity against multiple tumor cell lines. In the present study, we evaluated the expression level of PLK1 in OS and explored the cytotoxic mechanism of GSK461364 against OS. PLK1 was significantly overexpressed in OS compared with normal osteoblasts and other types of sarcoma. GSK461364 inhibited PLK1 and caused mitotic arrest by inducing G2/M arrest in OS cells. Moreover, GSK461364 exerted a cytotoxic effect by inducing apoptosis in OS, and induced cellular senescence in OS cell lines, as indicated by an increased senescence-associated β-galactosidase activity and enhanced DcR2 and interleukin-1α expression. In addition, we demonstrated a synergistic cytotoxic effect of GSK461364 and paclitaxel, possibly resulting from combined mitotic arrest. In conclusion, the present study revealed that PLK1 was overexpressed in OS and that GSK461364 exerted its cytotoxic effect on OS by inducing mitotic arrest and subsequent apoptosis and induced cellular senescence; therefore, senescence-associated markers can be used as treatment biomarkers, and a combination of GSK461364 and paclitaxel can potentially treat OS.
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View References

1 

Hung GY, Horng JL, Yen HJ, Yen CC, Chen WM, Chen PC, Wu HT and Chiou HJ: Incidence patterns of primary bone cancer in Taiwan (2003–2010): A population-based study. Ann Surg Oncol. 21:2490–2498. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Hung GY, Yen HJ, Yen CC, Chen WM, Chen PC, Wu HT, Chiou HJ, Chang WH and Hsu HE: Experience of pediatric osteosarcoma of the extremity at a single institution in Taiwan: Prognostic factors and impact on survival. Ann Surg Oncol. 22:1080–1087. 2015. View Article : Google Scholar

4 

Ferrari S, Smeland S, Mercuri M, Bertoni F, Longhi A, Ruggieri P, Alvegard TA, Picci P, Capanna R, Bernini G, et al; Italian and Scandinavian Sarcoma Groups. Neoadjuvant chemotherapy with high-dose Ifosfamide, high-dose methotrexate, cisplatin, and doxorubicin for patients with localized osteosarcoma of the extremity: A joint study by the Italian and Scandinavian Sarcoma Groups. J Clin Oncol. 23:8845–8852. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Iwamoto Y, Tanaka K, Isu K, Kawai A, Tatezaki S, Ishii T, Kushida K, Beppu Y, Usui M, Tateishi A, et al: Multiinstitutional phase II study of neoadjuvant chemotherapy for osteosarcoma (NECO study) in Japan: NECO-93J and NECO-95J. J Orthop Sci. 14:397–404. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Harting MT, Lally KP, Andrassy RJ, Vaporciyan AA, Cox CS Jr, Hayes-Jordan A and Blakely ML: Age as a prognostic factor for patients with osteosarcoma: An analysis of 438 patients. J Cancer Res Clin Oncol. 136:561–570. 2010. View Article : Google Scholar

7 

Joo MW, Shin SH, Kang YK, Kawai A, Kim HS, Asavamongkolkul A, Jeon DG, Kim JD, Niu X, Tsuchiya H, et al: Osteosarcoma in Asian populations over the age of 40 years: A Multicenter study. Ann Surg Oncol. 22:3557–3564. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Janeway KA, Barkauskas DA, Krailo MD, Meyers PA, Schwartz CL, Ebb DH, Seibel NL, Grier HE, Gorlick R and Marina N: Outcome for adolescent and young adult patients with osteosarcoma: A report from the Children's Oncology Group. Cancer. 118:4597–4605. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Whelan JS, Jinks RC, McTiernan A, Sydes MR, Hook JM, Trani L, Uscinska B, Bramwell V, Lewis IJ, Nooij MA, et al: Survival from high-grade localised extremity osteosarcoma: Combined results and prognostic factors from three European Osteosarcoma Intergroup randomised controlled trials. Ann Oncol. 23:1607–1616. 2012. View Article : Google Scholar :

10 

Mejia-Guerrero S, Quejada M, Gokgoz N, Gill M, Parkes RK, Wunder JS and Andrulis IL: Characterization of the 12q15 MDM2 and 12q13-14 CDK4 amplicons and clinical correlations in osteosarcoma. Genes Chromosomes Cancer. 49:518–525. 2010.PubMed/NCBI

11 

Crago AM, Socci ND, DeCarolis P, O'Connor R, Taylor BS, Qin LX, Antonescu CR and Singer S: Copy number losses define subgroups of dedifferentiated liposarcoma with poor prognosis and genomic instability. Clin Cancer Res. 18:1334–1340. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Wunder JS, Nielsen TO, Maki RG, O'Sullivan B and Alman BA: Opportunities for improving the therapeutic ratio for patients with sarcoma. Lancet Oncol. 8:513–524. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Ladanyi M, Cha C, Lewis R, Jhanwar SC, Huvos AG and Healey JH: MDM2 gene amplification in metastatic osteosarcoma. Cancer Res. 53:16–18. 1993.PubMed/NCBI

14 

Koshkina NV, Kleinerman ES, Li G, Zhao CC, Wei Q and Sturgis EM: Exploratory analysis of Fas gene polymorphisms in pediatric osteosarcoma patients. J Pediatr Hematol Oncol. 29:815–821. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Choy E, Hornicek F, MacConaill L, Harmon D, Tariq Z, Garraway L and Duan Z: High-throughput genotyping in osteosarcoma identifies multiple mutations in phosphoinositide-3-kinase and other oncogenes. Cancer. 118:2905–2914. 2012. View Article : Google Scholar :

16 

Perry JA, Kiezun A, Tonzi P, Van Allen EM, Carter SL, Baca SC, Cowley GS, Bhatt AS, Rheinbay E, Pedamallu CS, et al: Complementary genomic approaches highlight the PI3K/mTOR pathway as a common vulnerability in osteosarcoma. Proc Natl Acad Sci USA. 111:E5564–E5573. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Scheel C, Schaefer KL, Jauch A, Keller M, Wai D, Brinkschmidt C, van Valen F, Boecker W, Dockhorn-Dworniczak B and Poremba C: Alternative lengthening of telomeres is associated with chromosomal instability in osteosarcomas. Oncogene. 20:3835–3844. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Wan X, Mendoza A, Khanna C and Helman LJ: Rapamycin inhibits ezrin-mediated metastatic behavior in a murine model of osteosarcoma. Cancer Res. 65:2406–2411. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Yen CC, Chen WM, Chen TH, Chen WY, Chen PC, Chiou HJ, Hung GY, Wu HT, Wei CJ, Shiau CY, et al: Identification of chromosomal aberrations associated with disease progression and a novel 3q13.31 deletion involving LSAMP gene in osteosarcoma. Int J Oncol. 35:775–788. 2009.PubMed/NCBI

20 

Pasic I, Shlien A, Durbin AD, Stavropoulos DJ, Baskin B, Ray PN, Novokmet A and Malkin D: Recurrent focal copy-number changes and loss of heterozygosity implicate two noncoding RNAs and one tumor suppressor gene at chromosome 3q13.31 in osteosarcoma. Cancer Res. 70:160–171. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Grignani G, Palmerini E, Ferraresi V, D'Ambrosio L, Bertulli R, Asaftei SD, Tamburini A, Pignochino Y, Sangiolo D, Marchesi E, et al; Italian Sarcoma Group. Sorafenib and everolimus for patients with unresectable high-grade osteosarcoma progressing after standard treatment: A non-randomised phase 2 clinical trial. Lancet Oncol. 16:98–107. 2015. View Article : Google Scholar

22 

Strebhardt K and Ullrich A: Targeting polo-like kinase 1 for cancer therapy. Nat Rev Cancer. 6:321–330. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Duan Z, Ji D, Weinstein EJ, Liu X, Susa M, Choy E, Yang C, Mankin H and Hornicek FJ: Lentiviral shRNA screen of human kinases identifies PLK1 as a potential therapeutic target for osteosarcoma. Cancer Lett. 293:220–229. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Yamaguchi U, Honda K, Satow R, Kobayashi E, Nakayama R, Ichikawa H, Shoji A, Shitashige M, Masuda M, Kawai A, et al: Functional genome screen for therapeutic targets of osteosarcoma. Cancer Sci. 100:2268–2274. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Sero V, Tavanti E, Vella S, Hattinger CM, Fanelli M, Michelacci F, Versteeg R, Valsasina B, Gudeman B, Picci P, et al: Targeting polo-like kinase 1 by NMS-P937 in osteosarcoma cell lines inhibits tumor cell growth and partially overcomes drug resistance. Invest New Drugs. 32:1167–1180. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Yen CC, Hsiao CD, Chen WM, Wen YS, Lin YC, Chang TW, Yao FY, Hung SC, Wang JY, Chiu JH, et al: Cytotoxic effects of 15d-PGJ2 against osteosarcoma through ROS-mediated AKT and cell cycle inhibition. Oncotarget. 5:716–725. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Gilmartin AG, Bleam MR, Richter MC, Erskine SG, Kruger RG, Madden L, Hassler DF, Smith GK, Gontarek RR, Courtney MP, et al: Distinct concentration-dependent effects of the polo-like kinase 1-specific inhibitor GSK461364A, including differential effect on apoptosis. Cancer Res. 69:6969–6977. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Li C and Hung Wong W: Model-based analysis of oligonucleotide arrays: model validation, design issues and standard error application. Genome Biol. 2:Research0032. 2001.PubMed/NCBI

29 

Li C and Wong WH: Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection. Proc Natl Acad Sci USA. 98:31–36. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

31 

Chou TC and Talalay P: Quantitative analysis of dose-effect relationships: The combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul. 22:27–55. 1984. View Article : Google Scholar : PubMed/NCBI

32 

Schmit TL, Nihal M, Ndiaye M, Setaluri V, Spiegelman VS and Ahmad N: Numb regulates stability and localization of the mitotic kinase PLK1 and is required for transit through mitosis. Cancer Res. 72:3864–3872. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Tuveson DA, Willis NA, Jacks T, Griffin JD, Singer S, Fletcher CD, Fletcher JA and Demetri GD: STI571 inactivation of the gastrointestinal stromal tumor c-KIT oncoprotein: Biological and clinical implications. Oncogene. 20:5054–5058. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Zhu Y, Xu L, Zhang J, Hu X, Liu Y, Yin H, Lv T, Zhang H, Liu L, An H, et al: Sunitinib induces cellular senescence via p53/Dec1 activation in renal cell carcinoma cells. Cancer Sci. 104:1052–1061. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Müller-Tidow C, Metzger R, Kügler K, Diederichs S, Idos G, Thomas M, Dockhorn-Dworniczak B, Schneider PM, Koeffler HP, Berdel WE, et al: Cyclin E is the only cyclin-dependent kinase 2-associated cyclin that predicts metastasis and survival in early stage non-small cell lung cancer. Cancer Res. 61:647–653. 2001.PubMed/NCBI

36 

Ewald JA, Desotelle JA, Wilding G and Jarrard DF: Therapy-induced senescence in cancer. J Natl Cancer Inst. 102:1536–1546. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Chibon F, Lagarde P, Salas S, Pérot G, Brouste V, Tirode F, Lucchesi C, de Reynies A, Kauffmann A, Bui B, et al: Validated prediction of clinical outcome in sarcomas and multiple types of cancer on the basis of a gene expression signature related to genome complexity. Nat Med. 16:781–787. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Yen CC, Yeh CN, Cheng CT, Jung SM, Huang SC, Chang TW, Jan YY, Tzeng CH, Chao TC, Chen YY, et al: Integrating bioinformatics and clinicopathological research of gastrointestinal stromal tumors: Identification of aurora kinase A as a poor risk marker. Ann Surg Oncol. 19:3491–3499. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Yeh CN, Yen CC, Chen YY, Cheng CT, Huang SC, Chang TW, Yao FY, Lin YC, Wen YS, Chiang KC, et al: Identification of aurora kinase A as an unfavorable prognostic factor and potential treatment target for metastatic gastrointestinal stromal tumors. Oncotarget. 5:4071–4086. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Morales AG, Brassesco MS, Pezuk JA, Oliveira JC, Montaldi AP, Sakamoto-Hojo ET, Scrideli CA and Tone LG: BI 2536-mediated PLK1 inhibition suppresses HOS and MG-63 osteosarcoma cell line growth and clonogenicity. Anticancer Drugs. 22:995–1001. 2011.PubMed/NCBI

41 

Liu X, Choy E, Harmon D, Yang S, Yang C, Mankin H, Hornicek FJ and Duan Z: Inhibition of polo-like kinase 1 leads to the suppression of osteosarcoma cell growth in vitro and in vivo. Anticancer Drugs. 22:444–453. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Bogado RF, Pezuk JA, de Oliveira HF, Tone LG and Brassesco MS: BI 6727 and GSK461364 suppress growth and radiosensitize osteosarcoma cells, but show limited cytotoxic effects when combined with conventional treatments. Anticancer Drugs. 26:56–63. 2015. View Article : Google Scholar

43 

Spankuch B, Heim S, Kurunci-Csacsko E, Lindenau C, Yuan J, Kaufmann M and Strebhardt K: Down-regulation of Polo-like kinase 1 elevates drug sensitivity of breast cancer cells in vitro and in vivo. Cancer Res. 66:5836–5846. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chou Y, Yen C, Chen W, Lin Y, Wen Y, Ke W, Wang J, Liu C, Yang M, Chen T, Chen T, et al: Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel. Int J Oncol 48: 1187-1194, 2016.
APA
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W. ... Liu, C. (2016). Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel. International Journal of Oncology, 48, 1187-1194. https://doi.org/10.3892/ijo.2016.3352
MLA
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W., Wang, J., Liu, C., Yang, M., Chen, T., Liu, C."Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel". International Journal of Oncology 48.3 (2016): 1187-1194.
Chicago
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W., Wang, J., Liu, C., Yang, M., Chen, T., Liu, C."Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel". International Journal of Oncology 48, no. 3 (2016): 1187-1194. https://doi.org/10.3892/ijo.2016.3352
Copy and paste a formatted citation
x
Spandidos Publications style
Chou Y, Yen C, Chen W, Lin Y, Wen Y, Ke W, Wang J, Liu C, Yang M, Chen T, Chen T, et al: Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel. Int J Oncol 48: 1187-1194, 2016.
APA
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W. ... Liu, C. (2016). Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel. International Journal of Oncology, 48, 1187-1194. https://doi.org/10.3892/ijo.2016.3352
MLA
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W., Wang, J., Liu, C., Yang, M., Chen, T., Liu, C."Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel". International Journal of Oncology 48.3 (2016): 1187-1194.
Chicago
Chou, Y., Yen, C., Chen, W., Lin, Y., Wen, Y., Ke, W., Wang, J., Liu, C., Yang, M., Chen, T., Liu, C."Cytotoxic mechanism of PLK1 inhibitor GSK461364 against osteosarcoma: Mitotic arrest, apoptosis, cellular senescence, and synergistic effect with paclitaxel". International Journal of Oncology 48, no. 3 (2016): 1187-1194. https://doi.org/10.3892/ijo.2016.3352
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