Overexpression of KiSS-1 reduces colorectal cancer cell invasion by downregulating MMP-9 via blocking PI3K/Akt/NF-κB signal pathway
Affiliations: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
- Published online on: February 2, 2016 https://doi.org/10.3892/ijo.2016.3368
- Pages: 1391-1398
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Colorectal cancer (CRC) ranks the third most common cancer of all cancer types and is a major public health problem. In 2000, more than 1.2 million people were diagnosed with CRC (1), and according to the latest investigation in 2014, CRC is responsible for 8–9% of the cancer-related deaths in the USA (2). However, the incidence of CRC varies with geography with the incidence rate in the USA and Europe being 10-fold higher than in African and Asian countries. CRC is quite a complex disease and tumors with similar histopathological characteristics will finally develop with variable course in response to different treatments (3). As with all types of tumors, CRC is now proved to be a chronic systemic disease: neoplastic cells develop at tumor sites first and then metastasize to other parts of the body (4). Fortunately, advances in molecular biology in the last decades have facilitated the elucidation of some of the genetic mechanisms involved in the oncogenesis and development of CRC.
Three major mechanisms that cause aberrant gene expression results in the carcinogenesis of the colon: microsatellite instability (MSI), chromosomal instability (CIN), and the CpG island methylator phenotype (CIMP). These processes lead to the transition in lesion pathology and progression to malignancy, which is accompanied by downregulated expression of tumor suppression genes. Vogelstein et al have inferred that the accumulation of genetic alterations, in particular APC, TP53, and KRAS mutation is responsible for CRC development (5). Moreover, increased activity of matrix metal-loprotease-9 (MMP-9) was also detected in plasma of patients with colon cancer (6,7). MMP-9 is a matrix metalloproteinase, which is closely related multi-gene family of zinc-dependent proteolytic enzymes. It plays a role in normal physiological tissue remodeling and is capable of degrading all components of the extracellular matrix. Increasing evidence has proved the important contribution of MMP-9 to CRC (8,9), other studies have already revealed the underlying molecular mechanism involved in MMP-9 inducing cancer cell invasion (10). Taken the above information together, it is reasonable to consider MMP-9 as a potential therapeutic target for treatment of CRC.
A great deal of genes associated with metastases of cancer has been identified in the last decades. One of these genes is KiSS-1, expression of which is reported to be reduced in metastatic cancer (11). Moreover, its ability to suppress the metastatic potential of breast cancer cells without affecting tumorigenicity was also verified (11,12). Since MMP-9 has well-established role in tumor cell invasion and metastases, the interaction between MMP-9 and KiSS-1 has already drawn attention and the inhibition effect of KiSS-1 on the expression of MMP-9 has been validated (10). However, to the best of our knowledge, the mechanism by which KiSS-1 regulates MMP-9 and suppresses the metastatic phenotype remains partially explored, especially in CRC, the related studies are scarce.
Thus, in the present study, we hypothesized that KiSS-1 gene could repress the metastatic potential of colorectal cancer cells by downregulating the expression of MMP-9. Based on previous studies (10), we also investigated the possible molecular mechanism participating in these processes. Stable regulation of KiSS-1 gene in HCT-116 cells was achieved by lentivirus infection method. The effect of KiSS-1 on the cell viability, migration, and apoptosis was determined. The possible pathway involved in these processes was also evaluated by western blotting. Our aim was to assess the role of KiSS-1 and whether it was involved in oncogenesis and development of CRC to and facilitate the prevention and treatment of CRC in the clinic.
Materials and methods
Cell cultures and chemicals
Human CRC cell line HCT-116 was purchased from American Type Culture Collection (ATCC) (Rockville, MD, USA) and cultured in DMEM/F-12 medium supplemented with 10% (v/v) fetal calf serum (Gibco Life Technologies, Carlsbad, CA, USA) and 1% (v/v) anti-biotics mixture in 95% air and 5% CO2 at 37°C. In addition, cells between passage 3 and 6 were used for further experiments. PI3K/Akt pathway agonist 740Y-P and PDGF were purchased from Sigma-Aldrich, St. Louis, MO, USA.
siRNA interference of the KiSS-1 gene
Lentivirus-mediated KiSS-1-specific siRNA (5′-GCCGAACUACAACUGGA ACTT-3′) and the negative control siRNA (NC) were obtained from Genechem Biotech (Shanghai, China). The most effective transfection concentration of lentivirus was determined by multiplicity of infection (MOI). Based on the results (data not shown), lentivirus concentration at MOI 100 (10 μl 1×108 TU/ml lentivirus + 90 μl medium) was chosen for further experiments.
The cell concentration of HCT-116 was adjusted to 1×104/ml and incubated on slides in one well of 24-well plates for 24 h. Transfection was performed with Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol and subsequent selection was conducted using 400-μg/ml puromycin (Amresco, Solon, OH, USA). HCT-116 cells were grouped into two treatments: i) NC-siRNA group, HCT-116 cells were transfected with negative control siRNA. ii) KiSS-1-siRNA group, HCT-116 cells were transfected with KiSS-1-specific siRNA. Each treatment consisted of six replicates. All the cells were cultured for 96 h and cell sampling was conducted every 24 h during the experimental course from 0 h.
Overexpression of KiSS-1 gene in HCT-116 cells
Lentivirus-mediated KiSS-1 vector and negative control vector were purchased from Genechem (Shanghai, China). The most effective transfection concentration of lentivirus was determined by multiplicity of infection (MOI). Based on the results (data not shown), lentivirus concentration at MOI 100 (10 μl 1×108 TU/ml lentivirus + 90 μl medium) was chosen for further experiments. HCT-116 cells were grouped into two treatments and transfection was conducted as described above: i) control group, HCT-116 cells transfected with negative control vector. ii) KiSS-1 group, HCT-116 cells transfected with KiSS-1 vector. Each treatment consisted of six replicates. Cells were cultured for 96 h and sampling was conducted at 24, 48, 72, and 96 h.
Quantitative real-time PCR (qPCR)
For cell cultures from 24 h in different treatments, the whole RNA was extracted using TRIzol method according to the manufacturer's instructions. In addition, NAPDH was selected as the reference gene. The RNA was reverse transcribed to cDNA using RT-PCR kit (Fermentas China Co., Ltd., Shenzhen, China), and the final reaction mixture of volume 20 μl contained 10 μl of SYS BR Primix Ex Taq 2, 0.5 μl of each primers (KiSS-1, forward: 5′-AGCCGCCAGATCCCCGCA-3′; reverse: 5′-GCCGAA GGAGTTCCAGTTGTAGTT-3′. GAPDH, forward: 5′-GGG TGGAGCCAAACGGGTC-3′; reverse: 5′-GGAGTTGC TGTTGAAGTCGCA-3′), 1 μl of the cDNA template, and 8 μl ddH2O. Thermal cycling parameters for the amplification were as follows: a denaturation step at 94°C for 2 min, followed by 40 cycles at 94°C for 20 sec, 58°C for 30 sec and 72°C for 20 sec. Relative gene expression was evaluated with Data Assist software version 3.0 (Applied Biosystems, Foster City, CA, USA). The relative expression levels of KiSS-1 were determined according to the expression of 2−ΔΔct.
Western blot assay
Concentrations of protein samples were determined using the BCA method and western blot assay was performed as previously described (13); 20 μg of protein was subject to a 10% sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Then targeted proteins were transferred onto polyvinylidene difluoride (PVDF) sheets. The membranes were washed with TBST for 5 min and then transferred into blocking buffer for incubation overnight at 4°C. After three cycles of 5 min washes with TBST, primary antibodies (1:2000) against KiSS-1 or GAPDH were incubated with the membranes for 1 h at room temperature. After additional three washes, secondary HRP goat anti-rabbit IgG antibodies (1:2000) were added and incubated with the membranes for 40 min. After final three washes using TBST, the blots were developed using Beyo ECL Plus reagent and the results were detected in the Gel Imaging System. The relative expression levels of different proteins were calculated with Bio-Rad Quantity One.
Cell proliferation assay
For cell samples from 24, 48, 72, 96 h in both KiSS-1 interference and KiSS-1 overexpression treatments, cell viabilities were measured by CCK-8 method: briefly, CCK-8 solution was added to cells at different time points and cultured at 37°C for 90 min. OD value at 450 nm was determined using a Microplate Reader.
Transwell invasion assay
The transwell experiment to evaluate the invasion ability of HCT-116 cells was performed in cell samples from 96 h in different treatments: 100 μl of incubation medium (with 1 mM MgCl2) containing 1×105 cells were seeded into the upper chamber of BSA coated 8 μM pore size transwell chambers (Corning Star, Cambridge, MA, USA). Then cells were incubated at 37°C for 4 h to allow the migration through the porous membrane. The cells remaining at the upper surface of the chamber were completely removed. The lower surfaces of the membranes were stained in a solution containing 1% (w/v) crystal violet in 2% ethanol for 30 sec and then washed with ddH2O. Extraction of cell-associated crystal violet was performed by incubating in 10% acetic acid for 20 min. Results were observed using an Olympus CX41 microscope and the cell number in different treatments was determined using Image-Pro Plus 6.0 software (Nikon).
Fluorescence activated cell sorter
Effect of regulation of KiSS-1 gene on the apoptosis in HCT-116 cells was also determined using fluorescence activated cell sorter (FACS): 200 μl Annexin V/7-ADD working solution (5 μl Annexin V and 10 μl 7-AAD in 0.5 ml 1X Binding buffer) was added to cell samples from 96 h in different treatments. After incubation for 15 min at room temperature in the dark, the apoptotic rate was detected with flow cytometry. Each treatment was represented by three replicates. Apoptotic rate (UR+LR-all apoptosis cell percentage) was equal to the sum of the cell death rate (UR, upper right quadrant-advanced stage apoptosis cell percentage) and the early apoptosis rate (LR, lower right quadrant-prophase apoptosis cell percentage).
Detection of inhibition of KiSS-1 gene on PI3K/Akt/NF-κB- mediated MMP-9 expression
To further determine the molecular mechanism of KiSS-1 gene reducing the expression of MMP-9, we assessed the effect of KiSS-1 gene over-expression on PI3K/Akt/NF-κB signal transduction pathway. HCT-116 cells were classified into 4 groups: i) NC group, HCT-116 cells transfected with negative control lentivirus vector. ii) KiSS-1 group, HCT-116 cells transfected with lentivirus-mediated KiSS-1 vector. iii) KiSS-1 + 740Y-P group, KiSS-1 overexpression HCT-116 cells incubated with PI3K agonist 740Y-P (50 μg/ml) for 90 min. iv) KiSS-1 + PDGF group, KiSS-1 overexpression HCT-116 cells incubated with Akt agonist PDGF (100 ng/ml) for 1 h. Each treatment consisted of six replicates. All the cells were cultured for 96 h and sampled every 24 h. Cell viabilities of cultures from time points 24, 48, 72, and 96 h were measured using CCK-8 method as described above. In addition, cell invasion ability and apoptotic rate were determined using cell samples from 96 h. The expression of PI3K, Akt, pAkt, NF-κB subunit p65, and MMP-9 was determined with western blotting as described above.
The data are expressed as the mean ± SD. Student t-test and multiple comparisons with LSD method were conducted by using GLM model with significant level of p<0.05. All the statistical analysis were conducted using SPSS version 19.0 (IBM, Armonk, NY, USA).
Stable regulation of KiSS-1 gene in HCT-116 cells
Stable transfection of KiSS-1-specific siRNA and lentivirus-mediated KiSS-1 vector was detected using qPCR and western blotting (Figs. 1 and 2). The transfection of KiSS-1-specific siRNA significantly downregulated the transcription and synthesis of KiSS-1 (Fig. 1B and C), and the transfection of KiSS-1 vector significantly upregulated the expression of KiSS-1 in both mRNA and protein levels (Fig. 2B and C).
Expression of KiSS-1 after KiSS-1-specific siRNA transfection. (A) Stable transfection was achieved with lentivirus infection, MOI = 100. (B) Illustration of qPCR after KiSS-1-specific siRNA transfection. (C) Illustration of western blot KiSS-1-specific siRNA transfection. Significantly different from control group, *P<0.05.
Expression of KiSS-1 after KiSS-1 vector transfection. (A) Stable transfection was achieved with lentivirus infection, MOI=100. (B) Illustration of qPCR after KiSS-1 vector transfection. (C) Illustration of western blot after KiSS-1 vector transfection. Significantly different from control group, ***P<0.001.
Silencing of KiSS-1 gene by KiSS-1-specific siRNA has no impact on the cell viability, cell apoptosis, and invasion ability in HCT-116 cells
Although transfection with KiSS-1-specific siRNA influenced the transcription and production of KiSS-1, it seemed that silencing of KiSS-1 gene had no influence on the on the cell viability, cell invasion ability, or apoptosis within our experimental period (Figs. 3 and 4), which might indicate that the expression of KiSS-1 gene in normal HCT-116 cells was low and inhibition of the transcription of KiSS-1 gene had little influence on the regular biological activity of CRC cells.
Effect of KiSS-1-specific siRNA transfection on the cell proliferation and apoptosis of HCT-119 cells. (A) Influence of KiSS-1-specific siRNA transfection on the cell viability. (B) Influence of KiSS-1-specific siRNA transfection on the cell apoptosis. (C) Percentage of apoptotic cells evaluated by FACS.
Effect of KiSS-1-specific siRNA transfection on the invasion of HCT-119 cells. (A) The graph represents invasion ability of HCT-119 cells detected by transwell experiment. (B) Influence of KiSS-1 specific siRNA transfection on the invasion ability of HCT-119 cells.
Overexpression of KiSS-1 gene reduces the cell viability while induces cell apoptosis in HCT-116 cells
Overexpression of KiSS-1 gene led to a significant decrease in the cell proliferation in HCT-116 cells, especially at sampling points of 72 and 96 h, showing the potential of KiSS-1 as an effective anti-tumor target (Fig. 5A). Similar results were also observed in FACS, overexpression of KiSS-1 significantly increased the apoptotic rate (24.0%) in KiSS-1 group compared with NC group (7.1%) (Fig. 5B and C). Moreover, most non-viable cells in KiSS-1 group belonged to early apoptosis stage (18.9%).
Effect of KiSS-1 vector transfection on the cell proliferation and apoptosis of HCT-119 cells. (A) Influence of KiSS-1 vector transfection on the cell viability. (B) Influence of KiSS-1 vector transfection on cell apoptosis. (C) Percentage of apoptotic cells evaluated by FACS. Significantly different from control group, *P<0.05. Significantly different from control group, **P<0.01.
Overexpression of KiSS-1 gene decreases invasion ability in HCT-116 cells
The cell migration ability was evidently influenced by overexpression of KiSS-1 gene. To assess the function of KiSS-1 on cell mobility, we performed migration experiments in a modified Boyden chamber with HCT-116 cell transfected with different categories. Overexpression of KiSS-1 strongly reduced cell migration in KiSS-1 group (Fig. 6), however, given that the silencing of KiSS-1 had no significant effect on cell migration (Fig. 4), we inferred that KiSS-1 might exert its function in metastasis of CRC cells in an indirect manner.
Effect of KiSS-1 vector transfection on the invasion of HCT-119 cells. (A) The graph represents invasion ability of HCT-119 cells detected by transwell experiments. (B) Influence of KiSS-1 vector transfection on the invasion ability of HCT-119 cells. **P<0.01.
Employment of PI3K/Akt agonists attenuate the effect of KiSS-1 on the cell viability, cell apoptosis, and invasion ability in HCT-116 cells
To further explore the mechanism of KiSS-1 influencing the expression of MMP-9 and then reducing the metastasis of CRC cells, we set up another two groups, KiSS-1 overexpression HCT-116 cells incubated with PI3K agonist 740Y-P and Akt agonist PDGF, respectively. Treatments with the agonists significantly reversed the damage to HCT-116 cells. The cell viability in KiSS-1 + 740Y-P group and KiSS-1 + PDGF group was much higher than that in KiSS-1 group (Fig. 7A). The apoptotic rate and migration ability were also improved by incubation with the agonists (Figs. 7B and C, and 8). Although the biological condition of cells in these two groups was still poorer than NC group, the results indicated the evident involvement of PI3K/Akt pathway in KiSS-1 reducing proliferation and migration ability of HCT-116 cells.
KiSS-1 vector transfection reduces the cell proliferation and apoptosis of HCT-119 cells via PI3K/Akt pathway. (A) Influence of KiSS-1 vector transfection on the cell viability. (B) Influence of KiSS-1 vector transfection on cell apoptosis. (C) Percentage of apoptotic cells illustrated by FACS. Significantly different from vector, *P<0.05. Significantly different from vector, **P<0.01. Significantly different from Kiss-1 group, #P<0.05.
KiSS-1 vector transfection reduces the invasion of HCT-119 cells via PI3K/Akt pathway. (A) The graph represents invasion ability of HCT-119 cells detected by transwell experiments. (B) Influence of KiSS-1 vector transfection on the invasion ability of HCT-119 cells. Significantly different from vector, *P<0.05. Significantly different from vector, **P<0.01. Significantly different from Kiss-1 group, #P<0.05.
KiSS-1 downregulates the expression of MMP-9 by blocking PI3K/Akt/NF-κB pathway
We investigated the effect of KiSS-1 overexpression on the downstream activation by detecting the synthesis of MMP-9, PI3K, p65 and phosphorylation of Akt. Furthermore, to activate these pathways, we also treated KiSS-1 overexpression HCT-116 cells with PI3K/Akt agonists 740Y-P and PDGF. The results of western blotting clearly showed that the transfection with lentivirus-mediated KiSS-1 vector significantly reduced the synthesis of MMP-9, PI3K, pAkt, and p65 (Fig. 9). By co-incubation with different agonists, KiSS-1 overexpression-induced inhibition could be attenuated (Fig. 9). Taken the above information together, our results demonstrated that the decrease in cell invasion in CRC cells might result from the inhibition of MMP-9 due to KiSS-1 blocking the PI3K/Akt/NF-κB signal transduction pathway.
Effect of KiSS-1 protecting against CRC depends on the inhibition of the PI3K/Akt/NF-κB signal transduction pathway.
As proposed by Fearon and Vogelstein (14), CRC is a type of malignant neoplasm resulted from acquired and/or innate molecular alterations in colonic mucosa. Consumption of red meat and alcohol as well as obesity is associated with a higher risk of CRC. Moreover, inflammatory bowel patients also have greater risk for CRC development than health people. The genetic mutations and expression patterns of molecular markers involved in the oncogenesis of CRC and the in CRC are variable (15–17), which has led scientists to explore further the molecular level to meet the need for an accurate diagnosis, prognosis, and efficient therapeutic approach for CRC.
Cancer invasion and metastasis depend critically on the degradation of the extracellular matrix surrounding the tumor tissues. The process is believed to be activated by the action of proteolytic enzymes, including several types of MMPs (18). In the case of CRC, most studies have reported the increased expression of MMP-9 in patients with gastrointestinal cancers (8). Thus, regulation of MMP-9 has become a novel therapeutic target for prevention and treatment of CRC (8,9). Previous study of Yan et al (19) showed that KiSS-1 could repress MMP-9 in the human sarcoma cell line HT-1080, however, few studies have paid attention to the role of KiSS-1 in regulating MMP-9 in CRC, neither the related mechanisms. In the present study, we demonstrated that KiSS-1 gene could reduce the metastatic potential of colorectal cancer cells by downregulating the expression of MMP-9 and the interaction between KiSS-1 and MMP-9 was connected through the PI3K/Akt/NF-κB signal transduction pathway.
Our data elucidated that overexpression of KiSS-1 gene significantly suppressed the invasiveness and proliferation of in human CRC cell line HCT-116 while cell apoptosis was enhanced. Potential of migration and invasion is critical for tumor progression and metastasis. Lee and colleague have indicated that KiSS-1 inhibits invasiveness and metastasis of cancer cells (11). They found that loss of expression of KiSS-1 correlated with metastatic potential in human melanoma cells. This finding implies the therapeutic effect of KiSS-1 to reduce the migration ability of tumor cells. Our data derived from CRC model was different from that of the study by Lee et al (11): it was found that the silencing of KiSS-1 by specific siRNA had no influence on the cell viability, invasion, and apoptosis, although the transcription and synthesis of KiSS-1 in our experiment were significantly downregulated by RNAi technique. However, overexpression of KiSS-1 led to a significant decrease in cell invasion and proliferation. Thus, the regulation of KiSS-1 on the metastatic phenotype of different tumors is more complicated than expected.
Exposure to overexpression of KiSS-1 may induce the alteration of cellular signaling pathway mediators involved in the invasion of tumor cells, such as PI3K, MAPK, JNK, Akt, and NF-κB (10,20,21). However, previous studies showed that PI3K/Akt was one of the signal pathways to induce MMP-9 secretion and expression of KiSS-1 could reduce the NF-κB binding to the MMP-9 promoter (19,22). To elucidate the possible interactions between these processes, we investigated the overexpression of KiSS-1 on the synthesis of PI3K, Akt, pAkt, and NF-κB subunit p65. Our data showed that upregulation of KiSS-1 was associated with the inhibition of MMP-9, PI3K, pAkt, and p65. The PI3K/Akt and NF-κB signal transduction pathways are involved in the resistance of numerous solid tumors against a variety of anticancer drugs (23–25). By using array analysis, Agarwal et al (26) found that most genes of PI3K/Akt/IκB pathway were implicated in tumor angiogenesis and metastasis and positively regulating NF-κB and β-catenin in CRC cells.
In our experiment, we used agonists of PI3K and Akt to further detect the influence of KiSS-1 on this pathway. Both agonists activated the phosphorylation of Akt which was inhibited by the overexpression of KiSS-1, and then increased the expression of downstream p65 and MMP-9. However, Akt agonist PDGF had no effect on the expression of PI3K. Considering the regulating function of PI3K on Akt, this result confirmed that regulation of KiSS-1 on this pathway came into play in a PI3K to Akt pattern. Taken all the results together, we come to a conclusion that overexpression of KiSS-1 suppressed the invasiveness of CRC cells, mainly through downregulation of MMP-9 expression mediated by PI3K/Akt/NF-κB signal transduction pathway. This pathway also exits in other cell models: Yan et al demonstrated KiSS-1 could repress MMP-9 via reducing NF-κB binding to the MMP-9 promoter and Cheng et al showed that radiation enhanced the expression of MMP-9 via PI3K/Akt/NF-κB (10,19).
Collectively, our data revealed that overexpression of KiSS-1 could suppress the invasiveness of CRC cells, and the gene exerted its function by reducing the expression of MMP-9 by block of the PI3K/Akt/NF-κB pathway. The therapeutic effect of KiSS-1 depends not only on the elimination of local disease, but also on inhibiting systemic dissemination of CRC cells. The clarification of signal transduction mediators involved in this process might facilitate the development of specific inhibitors to modulate CRC metastatic signaling in the clinic.
The present study was supported by the National Key Clinical Specialties Construction Projects and the Key Project of Fujian Science and Technology Department (no. 2014y0021). The funders had no role in the study design, data collection and analysis, or preparation of the manuscript.
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