Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
May-2016 Volume 48 Issue 5

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2016 Volume 48 Issue 5

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling

  • Authors:
    • Kebang Hu
    • Yong Tian
    • Yanwei Du
    • Liandi Huang
    • Junyu Chen
    • Na Li
    • Wei Liu
    • Zuowen Liang
    • Lijing Zhao
  • View Affiliations / Copyright

    Affiliations: The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China, Jilin Academy of Environmental Science, Jilin, P.R. China
  • Pages: 2166-2174
    |
    Published online on: March 10, 2016
       https://doi.org/10.3892/ijo.2016.3433
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Atrazine, a widely used pesticide, is frequently detected in soil and surface water, which alarms epidemiologists and medical professionals because of its potential deleterious effects on health. Indeed, atrazine is a potent endocrine disruptor that increases aromatase expression in some human cancer cell lines. Both animal and human studies have suggested that atrazine is possibly carcinogenic, although discrepant results have been reported. In this study, RM1 cells were used to explore the atrazine effects on prostate cancer. Proliferation, migration and invasion of RM1 cells were assessed by colony formation, wound-healing and invasion assays, respectively, after in vitro exposure to atrazine. In addition, an RM1 cell xenograft model was generated to evaluate the effects of atrazine in vivo. To explore the molecular mechanisms, qRT‑PCR, immunohistochemistry, and western blot analyses were employed to detect mRNA and protein levels of STAT3 signaling and cell cycle related proteins, including p53, p21, cyclin B1 and cyclin D1. Interestingly, RM1 cell proliferation was increased after treatment with atrazine, concomitantly with STAT3 signaling activation. These results suggest that atrazine promotes RM1 cell growth in vitro and in vivo by activating STAT3 signaling.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Dong X, Zhu L, Wang J, Wang J, Xie H, Hou X and Jia W: Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio). Chemosphere. 77:404–412. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Lin Z, Fisher JW, Ross MK and Filipov NM: A physiologically based pharmacokinetic model for atrazine and its main metabolites in the adult male C57BL/6 mouse. Toxicol Appl Pharmacol. 251:16–31. 2011. View Article : Google Scholar

3 

Gerecke AC, Schärer M, Singer HP, Müller SR, Schwarzenbach RP, Sägesser M, Ochsenbein U and Popow G: Sources of pesticides in surface waters in Switzerland: Pesticide load through waste water treatment plants - current situation and reduction potential. Chemosphere. 48:307–315. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Ren J, Jiang K and Zhou H: The concentration and source of atrazine residue in water of Guanting reservoir. Huan Jing Ke Xue. 23:126–128. 2002.In Chinese. PubMed/NCBI

5 

Na T, Fang Z, Zhanqi G, Ming Z and Cheng S: The status of pesticide residues in the drinking water sources in Meiliangwan Bay, Taihu Lake of China. Environ Monit Assess. 123:351–370. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Rodriguez VM, Thiruchelvam M and Cory-Slechta DA: Sustained exposure to the widely used herbicide atrazine: Altered function and loss of neurons in brain monoamine systems. Environ Health Perspect. 113:708–715. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Coban A and Filipov NM: Dopaminergic toxicity associated with oral exposure to the herbicide atrazine in juvenile male C57BL/6 mice. J Neurochem. 100:1177–1187. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Cooper RL, Stoker TE, Tyrey L, Goldman JM and McElroy WK: Atrazine disrupts the hypothalamic control of pituitary-ovarian function. Toxicol Sci. 53:297–307. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Xing H, Wang C, Wu H, Chen D, Li S and Xu S: Effects of atrazine and chlorpyrifos on DNA methylation in the brain and gonad of the common carp. Comp Biochem Physiol C Toxicol Pharmacol. 168:11–19. 2015. View Article : Google Scholar

10 

Zhang X, Wang M, Gao S, Ren R, Zheng J and Zhang Y: Atrazine-induced apoptosis of splenocytes in BALB/C mice. BMC Med. 9:1172011. View Article : Google Scholar : PubMed/NCBI

11 

Boffetta P, Adami HO, Berry SC and Mandel JS: Atrazine and cancer: A review of the epidemiologic evidence. Eur J Cancer Prev. 22:169–180. 2013. View Article : Google Scholar

12 

Van Leeuwen JA, Waltner-Toews D, Abernathy T, Smit B and Shoukri M: Associations between stomach cancer incidence and drinking water contamination with atrazine and nitrate in Ontario (Canada) agroecosystems, 1987–1991. Int J Epidemiol. 28:836–840. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Kandori H, Suzuki S, Asamoto M, Murasaki T, Mingxi T, Ogawa K and Shirai T: Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats. Cancer Sci. 96:221–226. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Fan W, Yanase T, Morinaga H, Gondo S, Okabe T, Nomura M, Komatsu T, Morohashi K, Hayes TB, Takayanagi R, et al: Atrazine-induced aromatase expression is SF-1 dependent: Implications for endocrine disruption in wildlife and reproductive cancers in humans. Environ Health Perspect. 115:720–727. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Wang G, Niu X, Zhang W, Caldwell JT, Edwards H, Chen W, Taub JW, Zhao L and Ge Y: Synergistic antitumor interactions between MK-1775 and panobinostat in preclinical models of pancreatic cancer. Cancer Lett. 356B:656–668. 2015. View Article : Google Scholar

16 

Zhang Y, Hao H, Zhao S, Liu Q, Yuan Q, Ni S, Wang F, Liu S, Wang L and Hao A: Downregulation of GRIM-19 promotes growth and migration of human glioma cells. Cancer Sci. 102:1991–1999. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Hao H, Liu J, Liu G, Guan D, Yang Y, Zhang X, Cao X and Liu Q: Depletion of GRIM-19 accelerates hepatocellular carcinoma invasion via inducing EMT and loss of contact inhibition. J Cell Physiol. 227:1212–1219. 2012. View Article : Google Scholar

18 

Nallar SC, Kalakonda S, Sun P, Ohmori Y, Hiroi M, Mori K, Lindner DJ and Kalvakolanu DV: Identification of a structural motif in the tumor-suppressive protein GRIM-19 required for its antitumor activity. Am J Pathol. 177:896–907. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Huang Y, Yang M, Yang H and Zeng Z: Upregulation of the GRIM-19 gene suppresses invasion and metastasis of human gastric cancer SGC-7901 cell line. Exp Cell Res. 316:2061–2070. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Zhang L, Gao L, Li Y, Lin G, Shao Y, Ji K, Yu H, Hu J, Kalvakolanu DV, Kopecko DJ, et al: Effects of plasmid-based Stat3-specific short hairpin RNA and GRIM-19 on PC-3M tumor cell growth. Clin Cancer Res. 14:559–568. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Graymore M, Stagnitti F and Allinson G: Impacts of atrazine in aquatic ecosystems. Environ Int. 26:483–495. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Wang SC: PCNA: A silent housekeeper or a potential therapeutic target? Trends Pharmacol Sci. 35:178–186. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Yun HJ, Hyun SK, Park JH, Kim BW and Kwon HJ: Widdrol activates DNA damage checkpoint through the signaling Chk2-p53-Cdc25A-p21-MCM4 pathway in HT29 cells. Mol Cell Biochem. 363:281–289. 2012. View Article : Google Scholar

24 

Schick V, Majores M, Fassunke J, Engels G, Simon M, Elger CE and Becker AJ: Mutational and expression analysis of CDK1, cyclinA2 and cyclinB1 in epilepsy-associated glioneuronal lesions. Neuropathol Appl Neurobiol. 33:152–162. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Lee Y, Dominy JE, Choi YJ, Jurczak M, Tolliday N, Camporez JP, Chim H, Lim JH, Ruan HB, Yang X, et al: Cyclin D1-Cdk4 controls glucose metabolism independently of cell cycle progression. Nature. 510:547–551. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Lamb HM and Hardwick JM: Unlatched BAX pairs for death. Cell. 152:383–384. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Volkmann N, Marassi FM, Newmeyer DD and Hanein D: The rheostat in the membrane: BCL-2 family proteins and apoptosis. Cell Death Differ. 21:206–215. 2014. View Article : Google Scholar :

28 

Li SQ, Hu ZH, Zhu S, Wang DM, Han HM and Lu HJ: The effect of ADAM8 on the proliferation and apoptosis of hepatocytes and hepatoma carcinoma cells. J Biochem Mol Toxicol. 29:440–448. 2015. View Article : Google Scholar

29 

Baka S, Clamp AR and Jayson GC: A review of the latest clinical compounds to inhibit VEGF in pathological angiogenesis. Expert Opin Ther Targets. 10:867–876. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Cross MJ, Dixelius J, Matsumoto T and Claesson-Welsh L: VEGF-receptor signal transduction. Trends Biochem Sci. 28:488–494. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Li G, Zhang Y, Qian Y, Zhang H, Guo S, Sunagawa M, Hisamitsu T and Liu Y: Interleukin-17A promotes rheumatoid arthritis synoviocytes migration and invasion under hypoxia by increasing MMP2 and MMP9 expression through NF-κB/HIF-1α pathway. Mol Immunol. 53:227–236. 2013. View Article : Google Scholar

32 

Kalvakolanu DV, Nallar SC and Kalakonda S: Cytokine-induced tumor suppressors: A GRIM story. Cytokine. 52:128–142. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Moreira S, Correia M, Soares P and Máximo V: GRIM-19 function in cancer development. Mitochondrion. 11:693–699. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Liu YB, Zhang L, Guo YX, Gao LF, Liu XC, Zhao LJ, Guo BF, Zhao LJ, Zhao XJ and Xu DQ: Plasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth. Asian J Androl. 14:536–545. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Zhou T, Chao L, Rong G, Wang C, Ma R and Wang X: Down-regulation of GRIM-19 is associated with STAT3 overexpression in breast carcinomas. Hum Pathol. 44:1773–1779. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hu K, Tian Y, Du Y, Huang L, Chen J, Li N, Liu W, Liang Z and Zhao L: Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling. Int J Oncol 48: 2166-2174, 2016.
APA
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N. ... Zhao, L. (2016). Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling. International Journal of Oncology, 48, 2166-2174. https://doi.org/10.3892/ijo.2016.3433
MLA
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N., Liu, W., Liang, Z., Zhao, L."Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling". International Journal of Oncology 48.5 (2016): 2166-2174.
Chicago
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N., Liu, W., Liang, Z., Zhao, L."Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling". International Journal of Oncology 48, no. 5 (2016): 2166-2174. https://doi.org/10.3892/ijo.2016.3433
Copy and paste a formatted citation
x
Spandidos Publications style
Hu K, Tian Y, Du Y, Huang L, Chen J, Li N, Liu W, Liang Z and Zhao L: Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling. Int J Oncol 48: 2166-2174, 2016.
APA
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N. ... Zhao, L. (2016). Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling. International Journal of Oncology, 48, 2166-2174. https://doi.org/10.3892/ijo.2016.3433
MLA
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N., Liu, W., Liang, Z., Zhao, L."Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling". International Journal of Oncology 48.5 (2016): 2166-2174.
Chicago
Hu, K., Tian, Y., Du, Y., Huang, L., Chen, J., Li, N., Liu, W., Liang, Z., Zhao, L."Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling". International Journal of Oncology 48, no. 5 (2016): 2166-2174. https://doi.org/10.3892/ijo.2016.3433
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team