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Article

Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling

  • Authors:
    • Youchao Jia
    • Zhigang Wang
    • Aimin Zang
    • Shunchang Jiao
    • Sumei Chen
    • Yan Fu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, General Hospital of Chinese PLA, Beijing 100853, P.R. China, Department of Medical Oncology, Baoding Hengxing Hospital of Traditional Chinese and Western Medicine, Baoding 071000, P.R. China, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding 071000, P.R. China
  • Pages: 2079-2086
    |
    Published online on: March 15, 2016
       https://doi.org/10.3892/ijo.2016.3443
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Abstract

The underlying mechanisms of inhibitory effects induced by tetramethylpyrazine (TMP) on angiogenesis and tumor growth of lung cancer were investigated. In vitro cell proliferation, migration, and tube formation of human microvascular endothelial cells (HMEC-1) were evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT), wound healing, Transwell, and Matrigel assays. The expression of BMP/Smad/Id-1 signals was detected by RT-PCR and western blotting. In an A549 xenograft tumor model, TMP (40 and 80 mg/kg/day) was intraperitoneally injected into mice. The expressions of CD31, phosphorylated Smad1/5/8, and Id-1 were measured by immunohistochemistry. We demonstrated that TMP inhibited proliferation, migration, and capillary tube formation of HMEC-1 in a dose- and time-dependent manner. Furthermore, treatment of HMEC-1 cells with TMP (0.4 mg/ml) significantly upregulated BMP2 expression and downregulated BMPRIA, BMPRII, phosphorylated Smad1/5/8, and Id-1 expression. In addition, administrations of TMP remarkably inhibited tumor growth of A549 xenograft in nude mice. The CD31, phosphorylated Smad1/5/8, and Id-1 expression were significantly inhibited in TMP-treated xenograft tumors compared with the vehicle. In conclusion, our results indicated that TMP suppressed angiogenesis and tumor growth of lung cancer via blocking the BMP/Smad/Id-1 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Jia Y, Wang Z, Zang A, Jiao S, Chen S and Fu Y: Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling. Int J Oncol 48: 2079-2086, 2016.
APA
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., & Fu, Y. (2016). Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling. International Journal of Oncology, 48, 2079-2086. https://doi.org/10.3892/ijo.2016.3443
MLA
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., Fu, Y."Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling". International Journal of Oncology 48.5 (2016): 2079-2086.
Chicago
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., Fu, Y."Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling". International Journal of Oncology 48, no. 5 (2016): 2079-2086. https://doi.org/10.3892/ijo.2016.3443
Copy and paste a formatted citation
x
Spandidos Publications style
Jia Y, Wang Z, Zang A, Jiao S, Chen S and Fu Y: Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling. Int J Oncol 48: 2079-2086, 2016.
APA
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., & Fu, Y. (2016). Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling. International Journal of Oncology, 48, 2079-2086. https://doi.org/10.3892/ijo.2016.3443
MLA
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., Fu, Y."Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling". International Journal of Oncology 48.5 (2016): 2079-2086.
Chicago
Jia, Y., Wang, Z., Zang, A., Jiao, S., Chen, S., Fu, Y."Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling". International Journal of Oncology 48, no. 5 (2016): 2079-2086. https://doi.org/10.3892/ijo.2016.3443
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