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Article Open Access

Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells

Expression of Concern in: /10.3892/ijo.2025.5805
  • Authors:
    • Yunyan Wu
    • Qiang Liu
    • Xu Yan
    • Yukio Kato
    • Makiko Tanaka
    • Sadaki Inokuchi
    • Tadashi Yoshizawa
    • Satoko Morohashi
    • Hiroshi Kijima
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan, Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Science, Hiroshima 734-8553, Japan, Department of Critical Care and Emergency Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2310-2320
    |
    Published online on: March 18, 2016
       https://doi.org/10.3892/ijo.2016.3445
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Abstract

Podoplanin is reported involved in the collective cell invasion, another tumor invasion style which is distinct from the single cell invasion, so-called epithelial-mesenchymal transition (EMT). In this study, we investigated the correlation between podoplanin and EMT-related markers in esophageal squamous cell carcinoma (ESCC), and evaluated its linkage with the basic helix-loop-helix (bHLH) transcription factor differentiated embryonic chondrocyte (DEC) 1 and DEC2. Three ESCC cell lines and human squamous cell carcinoma A431 cells were subjected to western blot analyses for podoplanin and EMT markers, as well as the expression of DEC1 and DEC2. By RT-qPCR and western blotting, we found that TGF-β increased the expression of podoplanin and mensenchymal markers (e.g., N-cadherin and vimentin), while decreased the expression of epithelial markers (e.g., Claudin-4 and E-cadherin), accompanied by Smad2 phosphorylation and slug activation. Moreover, TGF-β has different effects on the expression of DEC1 and DEC2, that is, it upregulates DEC1, but downregulates DEC2. Capability of cell proliferation, invasion and migration were further analyzed using CCK-8 assay, Matrigel-invasion assay, and the wound-healing assay, respectively. The proliferation, invasion and migration ability were significantly lost in podoplanin-knockdown cells when compared with the scrambled siRNA group. In addition to these changes, the expression of Claudin-4, but not that of Claudin-1 or E-cadherin, was induced by the siRNA against podoplanin. On the contrary, overexpression of DEC1 and DEC2 exhibits opposite effects on podoplanin, but only slight effect on Claudin-4 was detected. These data indicated that podoplanin is significantly associated with EMT of TE-11 cells, and may be directly or indirectly regulated by bHLH transcription factors DEC1 and DEC2.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Liu Q, Yan X, Kato Y, Tanaka M, Inokuchi S, Yoshizawa T, Morohashi S and Kijima H: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805. Int J Oncol 48: 2310-2320, 2016.
APA
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S. ... Kijima, H. (2016). Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805. International Journal of Oncology, 48, 2310-2320. https://doi.org/10.3892/ijo.2016.3445
MLA
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S., Yoshizawa, T., Morohashi, S., Kijima, H."Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805". International Journal of Oncology 48.6 (2016): 2310-2320.
Chicago
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S., Yoshizawa, T., Morohashi, S., Kijima, H."Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805". International Journal of Oncology 48, no. 6 (2016): 2310-2320. https://doi.org/10.3892/ijo.2016.3445
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Liu Q, Yan X, Kato Y, Tanaka M, Inokuchi S, Yoshizawa T, Morohashi S and Kijima H: Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805. Int J Oncol 48: 2310-2320, 2016.
APA
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S. ... Kijima, H. (2016). Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805. International Journal of Oncology, 48, 2310-2320. https://doi.org/10.3892/ijo.2016.3445
MLA
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S., Yoshizawa, T., Morohashi, S., Kijima, H."Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805". International Journal of Oncology 48.6 (2016): 2310-2320.
Chicago
Wu, Y., Liu, Q., Yan, X., Kato, Y., Tanaka, M., Inokuchi, S., Yoshizawa, T., Morohashi, S., Kijima, H."Podoplanin-mediated TGF-β-induced epithelial-mesenchymal transition and its correlation with bHLH transcription factor DEC in TE-11 cells Expression of Concern in /10.3892/ijo.2025.5805". International Journal of Oncology 48, no. 6 (2016): 2310-2320. https://doi.org/10.3892/ijo.2016.3445
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